Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03642 and H281DRAFT_03643 overlap by 8 nucleotides H281DRAFT_03643 and H281DRAFT_03644 are separated by 41 nucleotides H281DRAFT_03644 and H281DRAFT_03645 are separated by 14 nucleotides H281DRAFT_03645 and H281DRAFT_03646 overlap by 8 nucleotides
H281DRAFT_03642: H281DRAFT_03642 - acetolactate synthase-1/2/3 large subunit, at 149,883 to 151,565
_03642
H281DRAFT_03643: H281DRAFT_03643 - aspartate dehydrogenase, at 151,558 to 152,388
_03643
H281DRAFT_03644: H281DRAFT_03644 - betaine-aldehyde dehydrogenase, at 152,430 to 153,929
_03644
H281DRAFT_03645: H281DRAFT_03645 - Predicted ring-cleavage extradiol dioxygenase, at 153,944 to 154,909
_03645
H281DRAFT_03646: H281DRAFT_03646 - 3-phenylpropionate/trans-cinnamate dioxygenase ferredoxin reductase subunit, at 154,902 to 156,167
_03646
Position (kb) 152
153
154 Strain fitness (log2 ratio) -1
0
1 at 151.702 kb on - strand, within H281DRAFT_03643 at 153.345 kb on + strand, within H281DRAFT_03644 at 153.781 kb on - strand at 153.781 kb on - strand at 153.975 kb on - strand at 154.449 kb on - strand, within H281DRAFT_03645 at 154.703 kb on + strand at 154.704 kb on - strand, within H281DRAFT_03645 at 154.740 kb on - strand, within H281DRAFT_03645
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 151,702 - H281DRAFT_03643 0.17 +0.4 153,345 + H281DRAFT_03644 0.61 -1.4 153,781 - +0.5 153,781 - +0.6 153,975 - +0.3 154,449 - H281DRAFT_03645 0.52 -1.0 154,703 + +1.5 154,704 - H281DRAFT_03645 0.79 +0.6 154,740 - H281DRAFT_03645 0.82 -0.4
Or see this region's nucleotide sequence