Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03477 and H281DRAFT_03478 are separated by 95 nucleotides H281DRAFT_03478 and H281DRAFT_03479 are separated by 194 nucleotides H281DRAFT_03479 and H281DRAFT_03480 are separated by 211 nucleotides
H281DRAFT_03477: H281DRAFT_03477 - hypothetical protein, at 559,557 to 560,510
_03477
H281DRAFT_03478: H281DRAFT_03478 - Predicted unusual protein kinase regulating ubiquinone biosynthesis, AarF/ABC1/UbiB family, at 560,606 to 562,165
_03478
H281DRAFT_03479: H281DRAFT_03479 - hypothetical protein, at 562,360 to 562,539
_03479
H281DRAFT_03480: H281DRAFT_03480 - 4-hydroxybenzoate polyprenyltransferase, at 562,751 to 564,172
_03480
Position (kb)
560
561
562
563 Strain fitness (log2 ratio)
-1
0
1
2 at 559.610 kb on + strand at 559.611 kb on - strand at 559.804 kb on + strand, within H281DRAFT_03477 at 559.804 kb on + strand, within H281DRAFT_03477 at 559.804 kb on + strand, within H281DRAFT_03477 at 559.888 kb on - strand, within H281DRAFT_03477 at 559.888 kb on - strand, within H281DRAFT_03477 at 559.935 kb on + strand, within H281DRAFT_03477 at 560.011 kb on - strand, within H281DRAFT_03477 at 560.011 kb on - strand, within H281DRAFT_03477 at 560.511 kb on + strand at 560.639 kb on + strand at 560.681 kb on - strand at 560.681 kb on - strand at 560.797 kb on + strand, within H281DRAFT_03478 at 560.798 kb on - strand, within H281DRAFT_03478 at 560.902 kb on + strand, within H281DRAFT_03478 at 561.481 kb on + strand, within H281DRAFT_03478 at 561.482 kb on - strand at 561.492 kb on - strand, within H281DRAFT_03478 at 562.556 kb on + strand at 562.866 kb on + strand at 562.952 kb on + strand, within H281DRAFT_03480 at 563.007 kb on + strand, within H281DRAFT_03480 at 563.013 kb on + strand, within H281DRAFT_03480 at 563.057 kb on + strand, within H281DRAFT_03480 at 563.058 kb on - strand, within H281DRAFT_03480
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 559,610 + +0.6 559,611 - -0.8 559,804 + H281DRAFT_03477 0.26 -0.7 559,804 + H281DRAFT_03477 0.26 +0.4 559,804 + H281DRAFT_03477 0.26 +0.2 559,888 - H281DRAFT_03477 0.35 +0.3 559,888 - H281DRAFT_03477 0.35 -1.3 559,935 + H281DRAFT_03477 0.40 +1.9 560,011 - H281DRAFT_03477 0.48 +0.3 560,011 - H281DRAFT_03477 0.48 +0.9 560,511 + -1.2 560,639 + +0.9 560,681 - -1.2 560,681 - +0.3 560,797 + H281DRAFT_03478 0.12 -0.2 560,798 - H281DRAFT_03478 0.12 -0.6 560,902 + H281DRAFT_03478 0.19 -0.2 561,481 + H281DRAFT_03478 0.56 +0.6 561,482 - +1.6 561,492 - H281DRAFT_03478 0.57 +0.2 562,556 + +0.4 562,866 + -0.1 562,952 + H281DRAFT_03480 0.14 -0.2 563,007 + H281DRAFT_03480 0.18 -0.2 563,013 + H281DRAFT_03480 0.18 -1.2 563,057 + H281DRAFT_03480 0.22 -0.0 563,058 - H281DRAFT_03480 0.22 -0.7
Or see this region's nucleotide sequence