Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03284 and H281DRAFT_03285 are separated by 24 nucleotides H281DRAFT_03285 and H281DRAFT_03286 overlap by 4 nucleotides H281DRAFT_03286 and H281DRAFT_03287 are separated by 110 nucleotides
H281DRAFT_03284: H281DRAFT_03284 - beta-1,4-N-acetylglucosamine oligosaccharide 6-O-carbamoyltransferase NodU, at 341,328 to 343,073
_03284
H281DRAFT_03285: H281DRAFT_03285 - lipopolysaccharide heptosyltransferase II, at 343,098 to 344,372
_03285
H281DRAFT_03286: H281DRAFT_03286 - D,D-heptose 1,7-bisphosphate phosphatase, at 344,369 to 344,974
_03286
H281DRAFT_03287: H281DRAFT_03287 - Glycosyltransferase involved in cell wall bisynthesis, at 345,085 to 346,380
_03287
Position (kb) 343
344
345 Strain fitness (log2 ratio) -1
0
1 at 342.398 kb on - strand, within H281DRAFT_03284 at 342.856 kb on + strand, within H281DRAFT_03284 at 342.856 kb on + strand, within H281DRAFT_03284 at 343.069 kb on + strand at 343.070 kb on - strand at 343.070 kb on - strand at 343.387 kb on + strand, within H281DRAFT_03285 at 343.388 kb on - strand, within H281DRAFT_03285 at 343.512 kb on - strand, within H281DRAFT_03285 at 343.797 kb on - strand, within H281DRAFT_03285 at 343.996 kb on + strand, within H281DRAFT_03285 at 344.957 kb on + strand at 345.177 kb on - strand at 345.177 kb on - strand at 345.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 342,398 - H281DRAFT_03284 0.61 +0.7 342,856 + H281DRAFT_03284 0.88 +0.0 342,856 + H281DRAFT_03284 0.88 -0.3 343,069 + -0.4 343,070 - -0.1 343,070 - +0.2 343,387 + H281DRAFT_03285 0.23 -1.1 343,388 - H281DRAFT_03285 0.23 +1.2 343,512 - H281DRAFT_03285 0.32 +0.4 343,797 - H281DRAFT_03285 0.55 -0.9 343,996 + H281DRAFT_03285 0.70 -0.4 344,957 + -1.6 345,177 - +1.0 345,177 - -0.0 345,177 - -0.4
Or see this region's nucleotide sequence