Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02815 and H281DRAFT_02816 overlap by 4 nucleotides H281DRAFT_02816 and H281DRAFT_02817 are separated by 207 nucleotides H281DRAFT_02817 and H281DRAFT_02818 are separated by 284 nucleotides H281DRAFT_02818 and H281DRAFT_02819 are separated by 220 nucleotides
H281DRAFT_02815: H281DRAFT_02815 - xanthine dehydrogenase YagS FAD-binding subunit, at 406,469 to 407,473
_02815
H281DRAFT_02816: H281DRAFT_02816 - xanthine dehydrogenase YagT iron-sulfur-binding subunit, at 407,470 to 408,012
_02816
H281DRAFT_02817: H281DRAFT_02817 - hypothetical protein, at 408,220 to 409,086
_02817
H281DRAFT_02818: H281DRAFT_02818 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 409,371 to 409,850
_02818
H281DRAFT_02819: H281DRAFT_02819 - DNA ligase-1, at 410,071 to 411,756
_02819
Position (kb) 408
409
410 Strain fitness (log2 ratio) -2
-1
0
1 at 407.544 kb on - strand, within H281DRAFT_02816 at 408.221 kb on + strand at 408.270 kb on - strand at 408.270 kb on - strand at 408.677 kb on + strand, within H281DRAFT_02817 at 408.678 kb on - strand, within H281DRAFT_02817 at 408.689 kb on + strand, within H281DRAFT_02817 at 408.689 kb on + strand, within H281DRAFT_02817 at 409.089 kb on - strand at 409.530 kb on - strand, within H281DRAFT_02818 at 409.854 kb on + strand at 409.854 kb on + strand at 409.854 kb on + strand at 410.050 kb on - strand at 410.050 kb on - strand at 410.053 kb on + strand at 410.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 407,544 - H281DRAFT_02816 0.14 +0.0 408,221 + -1.2 408,270 - +0.5 408,270 - +0.4 408,677 + H281DRAFT_02817 0.53 +0.7 408,678 - H281DRAFT_02817 0.53 +1.4 408,689 + H281DRAFT_02817 0.54 +0.6 408,689 + H281DRAFT_02817 0.54 +0.4 409,089 - +0.8 409,530 - H281DRAFT_02818 0.33 +0.1 409,854 + +1.3 409,854 + -2.1 409,854 + -0.3 410,050 - +0.3 410,050 - +0.8 410,053 + -1.8 410,054 - -1.3
Or see this region's nucleotide sequence