Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02228 and H281DRAFT_02229 overlap by 4 nucleotides H281DRAFT_02229 and H281DRAFT_02230 are separated by 203 nucleotides H281DRAFT_02230 and H281DRAFT_02231 are separated by 6 nucleotides H281DRAFT_02231 and H281DRAFT_02232 are separated by 72 nucleotides
H281DRAFT_02228: H281DRAFT_02228 - Sel1 repeat-containing protein, at 373,783 to 374,484
_02228
H281DRAFT_02229: H281DRAFT_02229 - 2-dehydro-3-deoxyglucarate aldolase, at 374,481 to 375,263
_02229
H281DRAFT_02230: H281DRAFT_02230 - 6-pyruvoyltetrahydropterin/6-carboxytetrahydropterin synthase, at 375,467 to 375,913
_02230
H281DRAFT_02231: H281DRAFT_02231 - 7-carboxy-7-deazaguanine synthase, Cx14CxxC type, at 375,920 to 376,552
_02231
H281DRAFT_02232: H281DRAFT_02232 - preQ(0) biosynthesis protein QueC, at 376,625 to 377,356
_02232
Position (kb) 375
376 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 374.553 kb on - strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.420 kb on + strand at 375.431 kb on + strand at 375.556 kb on - strand, within H281DRAFT_02230 at 375.822 kb on + strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.823 kb on - strand, within H281DRAFT_02230 at 375.842 kb on - strand, within H281DRAFT_02230 at 375.936 kb on + strand at 375.936 kb on + strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 375.937 kb on - strand at 376.048 kb on - strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.244 kb on + strand, within H281DRAFT_02231 at 376.522 kb on + strand at 376.522 kb on + strand at 376.522 kb on + strand at 376.523 kb on - strand at 376.523 kb on - strand at 376.524 kb on + strand at 376.525 kb on - strand at 376.574 kb on + strand at 376.749 kb on + strand, within H281DRAFT_02232 at 376.750 kb on - strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.756 kb on + strand, within H281DRAFT_02232 at 376.766 kb on + strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.767 kb on - strand, within H281DRAFT_02232 at 376.799 kb on + strand, within H281DRAFT_02232 at 376.800 kb on - strand, within H281DRAFT_02232 at 376.822 kb on + strand, within H281DRAFT_02232
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 374,553 - +0.3 375,420 + -0.1 375,420 + -0.1 375,420 + -0.4 375,420 + +0.5 375,431 + -0.9 375,556 - H281DRAFT_02230 0.20 +0.1 375,822 + H281DRAFT_02230 0.79 -0.4 375,823 - H281DRAFT_02230 0.80 -1.2 375,823 - H281DRAFT_02230 0.80 -0.4 375,823 - H281DRAFT_02230 0.80 -0.8 375,823 - H281DRAFT_02230 0.80 -2.5 375,842 - H281DRAFT_02230 0.84 +1.2 375,936 + -1.9 375,936 + +0.7 375,937 - +0.3 375,937 - -2.7 375,937 - -0.2 375,937 - -0.3 375,937 - +0.8 376,048 - H281DRAFT_02231 0.20 -2.2 376,244 + H281DRAFT_02231 0.51 -2.6 376,244 + H281DRAFT_02231 0.51 -0.2 376,522 + +0.0 376,522 + -0.4 376,522 + -1.2 376,523 - -0.0 376,523 - +0.4 376,524 + -0.8 376,525 - +0.5 376,574 + -0.3 376,749 + H281DRAFT_02232 0.17 -0.4 376,750 - H281DRAFT_02232 0.17 -1.8 376,756 + H281DRAFT_02232 0.18 +0.0 376,756 + H281DRAFT_02232 0.18 -0.1 376,756 + H281DRAFT_02232 0.18 -0.4 376,766 + H281DRAFT_02232 0.19 -1.1 376,767 - H281DRAFT_02232 0.19 -0.1 376,767 - H281DRAFT_02232 0.19 +0.2 376,767 - H281DRAFT_02232 0.19 -0.7 376,767 - H281DRAFT_02232 0.19 +0.2 376,799 + H281DRAFT_02232 0.24 -0.9 376,800 - H281DRAFT_02232 0.24 +3.6 376,822 + H281DRAFT_02232 0.27 -1.4
Or see this region's nucleotide sequence