Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02165 and H281DRAFT_02166 are separated by 274 nucleotides H281DRAFT_02166 and H281DRAFT_02167 are separated by 155 nucleotides H281DRAFT_02167 and H281DRAFT_02168 are separated by 242 nucleotides
H281DRAFT_02165: H281DRAFT_02165 - amidase, at 304,323 to 305,957
_02165
H281DRAFT_02166: H281DRAFT_02166 - High potential iron-sulfur protein, at 306,232 to 306,543
_02166
H281DRAFT_02167: H281DRAFT_02167 - L-2-hydroxyglutarate oxidase LhgO, at 306,699 to 307,805
_02167
H281DRAFT_02168: H281DRAFT_02168 - ATP-dependent Lon protease, at 308,048 to 309,013
_02168
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 305.738 kb on + strand, within H281DRAFT_02165 at 305.739 kb on - strand, within H281DRAFT_02165 at 305.812 kb on - strand at 305.812 kb on - strand at 305.939 kb on + strand at 305.939 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.956 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 306.090 kb on + strand at 306.091 kb on - strand at 306.180 kb on + strand at 306.180 kb on + strand at 306.246 kb on + strand at 306.247 kb on - strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.309 kb on - strand, within H281DRAFT_02166 at 306.333 kb on - strand, within H281DRAFT_02166 at 306.368 kb on + strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.413 kb on + strand, within H281DRAFT_02166 at 306.508 kb on - strand, within H281DRAFT_02166 at 306.560 kb on + strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.561 kb on - strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.563 kb on - strand at 306.563 kb on - strand at 306.604 kb on + strand at 306.604 kb on + strand at 306.605 kb on - strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.641 kb on - strand at 306.646 kb on + strand at 306.646 kb on + strand at 306.647 kb on - strand at 306.647 kb on - strand at 306.877 kb on + strand, within H281DRAFT_02167 at 306.877 kb on + strand, within H281DRAFT_02167 at 306.934 kb on + strand, within H281DRAFT_02167 at 306.967 kb on + strand, within H281DRAFT_02167 at 306.967 kb on + strand, within H281DRAFT_02167 at 307.111 kb on + strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.112 kb on - strand, within H281DRAFT_02167 at 307.129 kb on + strand, within H281DRAFT_02167 at 307.271 kb on + strand, within H281DRAFT_02167 at 307.271 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.448 kb on + strand, within H281DRAFT_02167 at 307.449 kb on - strand, within H281DRAFT_02167 at 307.565 kb on + strand at 307.565 kb on + strand, within H281DRAFT_02167 at 307.580 kb on - strand, within H281DRAFT_02167 at 307.585 kb on + strand, within H281DRAFT_02167 at 307.586 kb on - strand at 307.611 kb on + strand, within H281DRAFT_02167 at 307.612 kb on - strand, within H281DRAFT_02167 at 307.640 kb on - strand, within H281DRAFT_02167 at 307.642 kb on + strand, within H281DRAFT_02167 at 307.642 kb on + strand, within H281DRAFT_02167 at 307.642 kb on + strand at 307.642 kb on + strand, within H281DRAFT_02167 at 307.642 kb on + strand, within H281DRAFT_02167 at 307.642 kb on + strand, within H281DRAFT_02167 at 307.643 kb on - strand, within H281DRAFT_02167 at 307.643 kb on - strand, within H281DRAFT_02167 at 307.643 kb on - strand, within H281DRAFT_02167 at 307.643 kb on - strand, within H281DRAFT_02167 at 307.643 kb on - strand, within H281DRAFT_02167 at 307.782 kb on + strand at 307.852 kb on + strand at 307.852 kb on + strand at 307.852 kb on + strand at 307.852 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.854 kb on + strand at 307.855 kb on - strand at 307.855 kb on - strand at 307.855 kb on - strand at 307.855 kb on - strand at 307.855 kb on - strand at 307.855 kb on - strand at 307.887 kb on + strand at 307.887 kb on + strand at 307.887 kb on + strand at 307.888 kb on - strand at 307.942 kb on - strand at 308.014 kb on - strand at 308.050 kb on - strand at 308.262 kb on + strand, within H281DRAFT_02168 at 308.262 kb on + strand, within H281DRAFT_02168 at 308.460 kb on + strand, within H281DRAFT_02168 at 308.461 kb on - strand, within H281DRAFT_02168 at 308.481 kb on + strand, within H281DRAFT_02168 at 308.524 kb on + strand, within H281DRAFT_02168 at 308.524 kb on + strand, within H281DRAFT_02168 at 308.538 kb on + strand, within H281DRAFT_02168 at 308.538 kb on + strand, within H281DRAFT_02168 at 308.539 kb on - strand, within H281DRAFT_02168 at 308.539 kb on - strand, within H281DRAFT_02168 at 308.646 kb on + strand, within H281DRAFT_02168 at 308.653 kb on - strand, within H281DRAFT_02168
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 305,738 + H281DRAFT_02165 0.87 -0.8 305,739 - H281DRAFT_02165 0.87 +0.4 305,812 - -0.6 305,812 - -0.2 305,939 + +0.6 305,939 + +0.1 305,955 + +0.4 305,955 + +0.6 305,955 + +0.5 305,956 - -1.8 305,990 - -0.1 305,990 - +3.2 305,990 - -1.0 305,990 - -3.0 305,990 - +0.1 305,993 + +0.2 305,993 + +0.2 305,993 + -0.0 305,993 + +0.1 305,994 - +0.9 305,994 - +0.0 305,994 - -0.2 305,994 - -0.7 306,090 + -0.3 306,091 - -0.3 306,180 + +1.8 306,180 + +0.2 306,246 + +2.0 306,247 - -0.5 306,248 + +1.8 306,248 + +0.2 306,248 + -0.0 306,249 - -0.4 306,249 - -0.4 306,249 - +0.4 306,249 - -0.2 306,249 - +0.7 306,309 - H281DRAFT_02166 0.25 +1.7 306,333 - H281DRAFT_02166 0.32 +0.6 306,368 + H281DRAFT_02166 0.44 +1.1 306,369 - H281DRAFT_02166 0.44 +0.4 306,369 - H281DRAFT_02166 0.44 -0.8 306,413 + H281DRAFT_02166 0.58 +0.5 306,508 - H281DRAFT_02166 0.88 -1.0 306,560 + +1.1 306,560 + -0.0 306,560 + +2.0 306,561 - -1.6 306,562 + +1.2 306,562 + -0.3 306,562 + +0.2 306,563 - +0.5 306,563 - -1.1 306,604 + +0.2 306,604 + -0.0 306,605 - -0.7 306,621 + -0.1 306,621 + -0.6 306,621 + -0.6 306,621 + +1.3 306,641 - +0.0 306,646 + +0.4 306,646 + +0.1 306,647 - +0.1 306,647 - -3.4 306,877 + H281DRAFT_02167 0.16 -0.1 306,877 + H281DRAFT_02167 0.16 -0.0 306,934 + H281DRAFT_02167 0.21 +0.7 306,967 + H281DRAFT_02167 0.24 -0.6 306,967 + H281DRAFT_02167 0.24 -1.8 307,111 + H281DRAFT_02167 0.37 +0.2 307,112 - H281DRAFT_02167 0.37 +1.2 307,112 - H281DRAFT_02167 0.37 -0.1 307,112 - H281DRAFT_02167 0.37 -0.1 307,112 - H281DRAFT_02167 0.37 -0.8 307,112 - H281DRAFT_02167 0.37 +0.2 307,129 + H281DRAFT_02167 0.39 -0.3 307,271 + H281DRAFT_02167 0.52 +0.2 307,271 + H281DRAFT_02167 0.52 -1.1 307,448 + H281DRAFT_02167 0.68 -0.2 307,448 + H281DRAFT_02167 0.68 -2.2 307,448 + H281DRAFT_02167 0.68 +1.2 307,448 + H281DRAFT_02167 0.68 -1.1 307,449 - H281DRAFT_02167 0.68 +0.2 307,565 + +1.2 307,565 + H281DRAFT_02167 0.78 +0.1 307,580 - H281DRAFT_02167 0.80 -0.2 307,585 + H281DRAFT_02167 0.80 -1.4 307,586 - +4.2 307,611 + H281DRAFT_02167 0.82 -0.5 307,612 - H281DRAFT_02167 0.82 +2.0 307,640 - H281DRAFT_02167 0.85 -0.4 307,642 + H281DRAFT_02167 0.85 +1.3 307,642 + H281DRAFT_02167 0.85 +0.2 307,642 + +0.5 307,642 + H281DRAFT_02167 0.85 -0.7 307,642 + H281DRAFT_02167 0.85 +0.0 307,642 + H281DRAFT_02167 0.85 +0.4 307,643 - H281DRAFT_02167 0.85 +0.9 307,643 - H281DRAFT_02167 0.85 -1.3 307,643 - H281DRAFT_02167 0.85 +0.3 307,643 - H281DRAFT_02167 0.85 -0.5 307,643 - H281DRAFT_02167 0.85 -0.1 307,782 + +0.3 307,852 + -1.1 307,852 + +0.4 307,852 + -0.0 307,852 + -0.9 307,854 + -0.5 307,854 + +0.1 307,854 + +0.4 307,854 + -0.1 307,854 + -0.3 307,854 + -0.4 307,854 + -0.1 307,854 + +1.3 307,854 + +0.5 307,855 - -0.6 307,855 - -0.4 307,855 - +0.4 307,855 - -0.6 307,855 - +0.7 307,855 - -1.0 307,887 + +0.3 307,887 + -0.4 307,887 + -0.3 307,888 - +0.5 307,942 - -0.2 308,014 - -0.0 308,050 - +0.5 308,262 + H281DRAFT_02168 0.22 +0.9 308,262 + H281DRAFT_02168 0.22 -0.0 308,460 + H281DRAFT_02168 0.43 +0.2 308,461 - H281DRAFT_02168 0.43 +0.5 308,481 + H281DRAFT_02168 0.45 +0.5 308,524 + H281DRAFT_02168 0.49 +0.4 308,524 + H281DRAFT_02168 0.49 +0.3 308,538 + H281DRAFT_02168 0.51 +0.7 308,538 + H281DRAFT_02168 0.51 -0.1 308,539 - H281DRAFT_02168 0.51 -0.3 308,539 - H281DRAFT_02168 0.51 +1.9 308,646 + H281DRAFT_02168 0.62 +0.8 308,653 - H281DRAFT_02168 0.63 +0.4
Or see this region's nucleotide sequence