Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02164 and H281DRAFT_02165 are separated by 111 nucleotides H281DRAFT_02165 and H281DRAFT_02166 are separated by 274 nucleotides H281DRAFT_02166 and H281DRAFT_02167 are separated by 155 nucleotides
H281DRAFT_02164: H281DRAFT_02164 - carboxymethylenebutenolidase, at 303,513 to 304,211
_02164
H281DRAFT_02165: H281DRAFT_02165 - amidase, at 304,323 to 305,957
_02165
H281DRAFT_02166: H281DRAFT_02166 - High potential iron-sulfur protein, at 306,232 to 306,543
_02166
H281DRAFT_02167: H281DRAFT_02167 - L-2-hydroxyglutarate oxidase LhgO, at 306,699 to 307,805
_02167
Position (kb)
304
305
306 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 303.500 kb on + strand at 303.645 kb on - strand, within H281DRAFT_02164 at 303.645 kb on - strand, within H281DRAFT_02164 at 303.645 kb on - strand, within H281DRAFT_02164 at 303.666 kb on + strand, within H281DRAFT_02164 at 303.681 kb on + strand, within H281DRAFT_02164 at 303.681 kb on + strand, within H281DRAFT_02164 at 303.681 kb on + strand, within H281DRAFT_02164 at 303.682 kb on - strand, within H281DRAFT_02164 at 303.683 kb on + strand, within H281DRAFT_02164 at 303.683 kb on + strand, within H281DRAFT_02164 at 303.684 kb on - strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.738 kb on + strand, within H281DRAFT_02164 at 303.739 kb on - strand, within H281DRAFT_02164 at 303.739 kb on - strand, within H281DRAFT_02164 at 303.739 kb on - strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.900 kb on + strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.901 kb on - strand, within H281DRAFT_02164 at 303.902 kb on + strand, within H281DRAFT_02164 at 303.902 kb on + strand, within H281DRAFT_02164 at 303.903 kb on - strand, within H281DRAFT_02164 at 303.903 kb on - strand, within H281DRAFT_02164 at 303.943 kb on - strand, within H281DRAFT_02164 at 303.946 kb on - strand, within H281DRAFT_02164 at 304.012 kb on - strand, within H281DRAFT_02164 at 304.101 kb on + strand, within H281DRAFT_02164 at 304.102 kb on - strand, within H281DRAFT_02164 at 304.146 kb on + strand at 304.147 kb on - strand at 304.149 kb on - strand at 304.209 kb on + strand at 304.209 kb on + strand at 304.380 kb on - strand at 304.424 kb on - strand at 304.431 kb on + strand at 304.431 kb on + strand at 304.431 kb on + strand at 304.432 kb on - strand at 304.566 kb on + strand, within H281DRAFT_02165 at 304.567 kb on - strand, within H281DRAFT_02165 at 304.567 kb on - strand, within H281DRAFT_02165 at 304.689 kb on + strand, within H281DRAFT_02165 at 304.860 kb on + strand, within H281DRAFT_02165 at 304.860 kb on + strand, within H281DRAFT_02165 at 304.862 kb on + strand, within H281DRAFT_02165 at 304.862 kb on + strand, within H281DRAFT_02165 at 304.862 kb on + strand, within H281DRAFT_02165 at 304.863 kb on - strand, within H281DRAFT_02165 at 304.863 kb on - strand, within H281DRAFT_02165 at 304.863 kb on - strand, within H281DRAFT_02165 at 304.913 kb on + strand, within H281DRAFT_02165 at 304.914 kb on - strand, within H281DRAFT_02165 at 304.914 kb on - strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.241 kb on + strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.242 kb on - strand, within H281DRAFT_02165 at 305.334 kb on - strand, within H281DRAFT_02165 at 305.738 kb on + strand, within H281DRAFT_02165 at 305.739 kb on - strand, within H281DRAFT_02165 at 305.812 kb on - strand at 305.812 kb on - strand at 305.939 kb on + strand at 305.939 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.955 kb on + strand at 305.956 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.990 kb on - strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.993 kb on + strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 305.994 kb on - strand at 306.090 kb on + strand at 306.091 kb on - strand at 306.180 kb on + strand at 306.180 kb on + strand at 306.246 kb on + strand at 306.247 kb on - strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.248 kb on + strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.249 kb on - strand at 306.309 kb on - strand, within H281DRAFT_02166 at 306.333 kb on - strand, within H281DRAFT_02166 at 306.368 kb on + strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.369 kb on - strand, within H281DRAFT_02166 at 306.413 kb on + strand, within H281DRAFT_02166 at 306.508 kb on - strand, within H281DRAFT_02166 at 306.560 kb on + strand at 306.560 kb on + strand at 306.560 kb on + strand at 306.561 kb on - strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.562 kb on + strand at 306.563 kb on - strand at 306.563 kb on - strand at 306.604 kb on + strand at 306.604 kb on + strand at 306.605 kb on - strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.621 kb on + strand at 306.641 kb on - strand at 306.646 kb on + strand at 306.646 kb on + strand at 306.647 kb on - strand at 306.647 kb on - strand at 306.877 kb on + strand, within H281DRAFT_02167 at 306.877 kb on + strand, within H281DRAFT_02167 at 306.934 kb on + strand, within H281DRAFT_02167
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 303,500 + -0.4 303,645 - H281DRAFT_02164 0.19 +0.4 303,645 - H281DRAFT_02164 0.19 +0.8 303,645 - H281DRAFT_02164 0.19 +2.8 303,666 + H281DRAFT_02164 0.22 +1.5 303,681 + H281DRAFT_02164 0.24 -0.5 303,681 + H281DRAFT_02164 0.24 -0.5 303,681 + H281DRAFT_02164 0.24 -1.0 303,682 - H281DRAFT_02164 0.24 -0.1 303,683 + H281DRAFT_02164 0.24 +0.2 303,683 + H281DRAFT_02164 0.24 -1.6 303,684 - H281DRAFT_02164 0.24 +0.4 303,738 + H281DRAFT_02164 0.32 +0.2 303,738 + H281DRAFT_02164 0.32 -0.4 303,738 + H281DRAFT_02164 0.32 +0.7 303,738 + H281DRAFT_02164 0.32 -0.3 303,738 + -0.8 303,738 + H281DRAFT_02164 0.32 +1.1 303,738 + H281DRAFT_02164 0.32 -0.5 303,738 + H281DRAFT_02164 0.32 +0.6 303,739 - H281DRAFT_02164 0.32 -0.2 303,739 - H281DRAFT_02164 0.32 +0.1 303,739 - H281DRAFT_02164 0.32 -0.8 303,900 + H281DRAFT_02164 0.55 -0.4 303,900 + H281DRAFT_02164 0.55 +0.1 303,900 + H281DRAFT_02164 0.55 +0.6 303,900 + H281DRAFT_02164 0.55 -1.1 303,900 + H281DRAFT_02164 0.55 -0.6 303,900 + H281DRAFT_02164 0.55 +0.3 303,900 + H281DRAFT_02164 0.55 +0.3 303,900 + H281DRAFT_02164 0.55 -0.4 303,900 + H281DRAFT_02164 0.55 -0.1 303,900 + H281DRAFT_02164 0.55 +0.1 303,900 + H281DRAFT_02164 0.55 -0.0 303,900 + H281DRAFT_02164 0.55 -0.4 303,900 + H281DRAFT_02164 0.55 -3.0 303,901 - H281DRAFT_02164 0.56 +0.2 303,901 - H281DRAFT_02164 0.56 +0.5 303,901 - H281DRAFT_02164 0.56 +0.4 303,901 - H281DRAFT_02164 0.56 -0.1 303,901 - H281DRAFT_02164 0.56 -0.0 303,901 - H281DRAFT_02164 0.56 -1.6 303,901 - H281DRAFT_02164 0.56 +0.1 303,901 - H281DRAFT_02164 0.56 +0.1 303,901 - H281DRAFT_02164 0.56 -1.3 303,901 - +1.2 303,901 - H281DRAFT_02164 0.56 -0.9 303,901 - H281DRAFT_02164 0.56 -0.7 303,901 - H281DRAFT_02164 0.56 +0.5 303,901 - H281DRAFT_02164 0.56 +0.2 303,901 - H281DRAFT_02164 0.56 -1.5 303,901 - H281DRAFT_02164 0.56 +1.1 303,902 + H281DRAFT_02164 0.56 +0.2 303,902 + H281DRAFT_02164 0.56 +0.5 303,903 - H281DRAFT_02164 0.56 -0.4 303,903 - H281DRAFT_02164 0.56 +1.2 303,943 - H281DRAFT_02164 0.62 -0.3 303,946 - H281DRAFT_02164 0.62 -0.3 304,012 - H281DRAFT_02164 0.71 -0.7 304,101 + H281DRAFT_02164 0.84 -0.1 304,102 - H281DRAFT_02164 0.84 +0.4 304,146 + -0.1 304,147 - -0.2 304,149 - -0.3 304,209 + -0.7 304,209 + -0.9 304,380 - +0.3 304,424 - -0.5 304,431 + -0.4 304,431 + +0.4 304,431 + -0.5 304,432 - -0.2 304,566 + H281DRAFT_02165 0.15 -0.9 304,567 - H281DRAFT_02165 0.15 -0.1 304,567 - H281DRAFT_02165 0.15 -0.0 304,689 + H281DRAFT_02165 0.22 -0.7 304,860 + H281DRAFT_02165 0.33 +0.1 304,860 + H281DRAFT_02165 0.33 -0.0 304,862 + H281DRAFT_02165 0.33 +0.9 304,862 + H281DRAFT_02165 0.33 -0.2 304,862 + H281DRAFT_02165 0.33 -0.1 304,863 - H281DRAFT_02165 0.33 -0.2 304,863 - H281DRAFT_02165 0.33 +0.8 304,863 - H281DRAFT_02165 0.33 +1.2 304,913 + H281DRAFT_02165 0.36 +0.1 304,914 - H281DRAFT_02165 0.36 -0.1 304,914 - H281DRAFT_02165 0.36 -1.0 305,241 + H281DRAFT_02165 0.56 +0.4 305,241 + H281DRAFT_02165 0.56 +0.0 305,241 + H281DRAFT_02165 0.56 +0.4 305,241 + H281DRAFT_02165 0.56 -0.4 305,241 + H281DRAFT_02165 0.56 -0.6 305,242 - H281DRAFT_02165 0.56 +1.7 305,242 - H281DRAFT_02165 0.56 +1.5 305,242 - H281DRAFT_02165 0.56 +0.8 305,334 - H281DRAFT_02165 0.62 -0.7 305,738 + H281DRAFT_02165 0.87 -0.8 305,739 - H281DRAFT_02165 0.87 +0.4 305,812 - -0.6 305,812 - -0.2 305,939 + +0.6 305,939 + +0.1 305,955 + +0.4 305,955 + +0.6 305,955 + +0.5 305,956 - -1.8 305,990 - -0.1 305,990 - +3.2 305,990 - -1.0 305,990 - -3.0 305,990 - +0.1 305,993 + +0.2 305,993 + +0.2 305,993 + -0.0 305,993 + +0.1 305,994 - +0.9 305,994 - +0.0 305,994 - -0.2 305,994 - -0.7 306,090 + -0.3 306,091 - -0.3 306,180 + +1.8 306,180 + +0.2 306,246 + +2.0 306,247 - -0.5 306,248 + +1.8 306,248 + +0.2 306,248 + -0.0 306,249 - -0.4 306,249 - -0.4 306,249 - +0.4 306,249 - -0.2 306,249 - +0.7 306,309 - H281DRAFT_02166 0.25 +1.7 306,333 - H281DRAFT_02166 0.32 +0.6 306,368 + H281DRAFT_02166 0.44 +1.1 306,369 - H281DRAFT_02166 0.44 +0.4 306,369 - H281DRAFT_02166 0.44 -0.8 306,413 + H281DRAFT_02166 0.58 +0.5 306,508 - H281DRAFT_02166 0.88 -1.0 306,560 + +1.1 306,560 + -0.0 306,560 + +2.0 306,561 - -1.6 306,562 + +1.2 306,562 + -0.3 306,562 + +0.2 306,563 - +0.5 306,563 - -1.1 306,604 + +0.2 306,604 + -0.0 306,605 - -0.7 306,621 + -0.1 306,621 + -0.6 306,621 + -0.6 306,621 + +1.3 306,641 - +0.0 306,646 + +0.4 306,646 + +0.1 306,647 - +0.1 306,647 - -3.4 306,877 + H281DRAFT_02167 0.16 -0.1 306,877 + H281DRAFT_02167 0.16 -0.0 306,934 + H281DRAFT_02167 0.21 +0.7
Or see this region's nucleotide sequence