Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01318 and H281DRAFT_01319 are separated by 54 nucleotides H281DRAFT_01319 and H281DRAFT_01320 are separated by 93 nucleotides H281DRAFT_01320 and H281DRAFT_01321 are separated by 103 nucleotides
H281DRAFT_01318: H281DRAFT_01318 - hypothetical protein, at 159,218 to 159,802
_01318
H281DRAFT_01319: H281DRAFT_01319 - Uncharacterized protein, UPF0264 family, at 159,857 to 160,600
_01319
H281DRAFT_01320: H281DRAFT_01320 - Uncharacterized archaeal kinase related to aspartokinases, uridylate kinases, at 160,694 to 161,284
_01320
H281DRAFT_01321: H281DRAFT_01321 - probable H4MPT-linked C1 transfer pathway protein, at 161,388 to 162,512
_01321
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2 at 158.918 kb on + strand at 158.919 kb on - strand at 159.018 kb on - strand at 159.018 kb on - strand at 159.043 kb on - strand at 159.100 kb on + strand at 159.100 kb on + strand at 159.116 kb on - strand at 159.242 kb on - strand at 159.346 kb on + strand, within H281DRAFT_01318 at 159.347 kb on - strand, within H281DRAFT_01318 at 159.811 kb on - strand at 160.118 kb on + strand, within H281DRAFT_01319 at 160.598 kb on + strand at 160.599 kb on - strand at 160.634 kb on - strand at 160.747 kb on + strand at 160.747 kb on + strand at 160.767 kb on + strand, within H281DRAFT_01320
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 158,918 + +0.1 158,919 - -0.3 159,018 - +0.3 159,018 - +0.8 159,043 - +0.1 159,100 + -1.0 159,100 + +1.7 159,116 - -0.3 159,242 - -0.9 159,346 + H281DRAFT_01318 0.22 +0.7 159,347 - H281DRAFT_01318 0.22 +1.1 159,811 - -0.6 160,118 + H281DRAFT_01319 0.35 +0.2 160,598 + +0.5 160,599 - +0.9 160,634 - -0.5 160,747 + -2.2 160,747 + +1.9 160,767 + H281DRAFT_01320 0.12 -0.6
Or see this region's nucleotide sequence