Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01158 and H281DRAFT_01159 are separated by 63 nucleotides H281DRAFT_01159 and H281DRAFT_01160 are separated by 84 nucleotides H281DRAFT_01160 and H281DRAFT_01161 are separated by 266 nucleotides
H281DRAFT_01158: H281DRAFT_01158 - 4-deoxy-L-threo-5-hexulose uronate isomerase, at 1,307,803 to 1,308,639
_01158
H281DRAFT_01159: H281DRAFT_01159 - 2-deoxy-D-gluconate 3-dehydrogenase, at 1,308,703 to 1,309,503
_01159
H281DRAFT_01160: H281DRAFT_01160 - transcriptional regulator, IclR family, at 1,309,588 to 1,310,463
_01160
H281DRAFT_01161: H281DRAFT_01161 - cytochrome b561, at 1,310,730 to 1,311,284
_01161
Position (kb)
1309
1310
1311 Strain fitness (log2 ratio)
-1
0
1 at 1308.657 kb on - strand at 1309.026 kb on - strand, within H281DRAFT_01159 at 1309.213 kb on + strand, within H281DRAFT_01159 at 1309.309 kb on + strand, within H281DRAFT_01159 at 1309.310 kb on - strand, within H281DRAFT_01159 at 1309.454 kb on - strand at 1309.454 kb on - strand at 1309.468 kb on - strand at 1309.644 kb on - strand at 1309.853 kb on - strand, within H281DRAFT_01160 at 1310.002 kb on + strand, within H281DRAFT_01160 at 1310.003 kb on - strand, within H281DRAFT_01160 at 1310.366 kb on - strand, within H281DRAFT_01160 at 1310.691 kb on - strand at 1310.834 kb on - strand, within H281DRAFT_01161 at 1310.842 kb on - strand, within H281DRAFT_01161 at 1311.175 kb on - strand, within H281DRAFT_01161 at 1311.235 kb on + strand at 1311.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 1,308,657 - -0.6 1,309,026 - H281DRAFT_01159 0.40 +0.2 1,309,213 + H281DRAFT_01159 0.64 +0.2 1,309,309 + H281DRAFT_01159 0.76 -1.2 1,309,310 - H281DRAFT_01159 0.76 +0.9 1,309,454 - -1.1 1,309,454 - -0.1 1,309,468 - +0.5 1,309,644 - -0.5 1,309,853 - H281DRAFT_01160 0.30 +0.1 1,310,002 + H281DRAFT_01160 0.47 +0.2 1,310,003 - H281DRAFT_01160 0.47 -0.7 1,310,366 - H281DRAFT_01160 0.89 +1.0 1,310,691 - -1.2 1,310,834 - H281DRAFT_01161 0.19 +0.3 1,310,842 - H281DRAFT_01161 0.20 +1.4 1,311,175 - H281DRAFT_01161 0.80 -0.7 1,311,235 + -1.7 1,311,346 - +0.5
Or see this region's nucleotide sequence