Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01151 and H281DRAFT_01152 overlap by 4 nucleotides H281DRAFT_01152 and H281DRAFT_01153 are separated by 227 nucleotides H281DRAFT_01153 and H281DRAFT_01154 are separated by 111 nucleotides
H281DRAFT_01151: H281DRAFT_01151 - ceramide glucosyltransferase, at 1,300,468 to 1,301,634
_01151
H281DRAFT_01152: H281DRAFT_01152 - hopanoid biosynthesis associated radical SAM protein HpnJ, at 1,301,631 to 1,303,052
_01152
H281DRAFT_01153: H281DRAFT_01153 - hypothetical protein, at 1,303,280 to 1,303,660
_01153
H281DRAFT_01154: H281DRAFT_01154 - cold-shock DNA-binding protein family, at 1,303,772 to 1,303,975
_01154
Position (kb)
1301
1302
1303
1304 Strain fitness (log2 ratio)
-1
0
1 at 1301.184 kb on - strand, within H281DRAFT_01151 at 1301.412 kb on - strand, within H281DRAFT_01151 at 1301.485 kb on + strand, within H281DRAFT_01151 at 1301.598 kb on - strand at 1301.765 kb on - strand at 1301.765 kb on - strand at 1301.972 kb on - strand, within H281DRAFT_01152 at 1302.956 kb on - strand at 1303.021 kb on + strand at 1303.412 kb on - strand, within H281DRAFT_01153 at 1303.609 kb on - strand, within H281DRAFT_01153
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 1,301,184 - H281DRAFT_01151 0.61 +0.4 1,301,412 - H281DRAFT_01151 0.81 +0.5 1,301,485 + H281DRAFT_01151 0.87 +0.8 1,301,598 - -0.6 1,301,765 - -0.0 1,301,765 - +0.4 1,301,972 - H281DRAFT_01152 0.24 -0.1 1,302,956 - -0.2 1,303,021 + -0.5 1,303,412 - H281DRAFT_01153 0.35 +0.2 1,303,609 - H281DRAFT_01153 0.86 +1.1
Or see this region's nucleotide sequence