Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00009 and H281DRAFT_00010 are separated by 300 nucleotides H281DRAFT_00010 and H281DRAFT_00011 overlap by 4 nucleotides H281DRAFT_00011 and H281DRAFT_00012 are separated by 1 nucleotides H281DRAFT_00012 and H281DRAFT_00013 are separated by 584 nucleotides
H281DRAFT_00009: H281DRAFT_00009 - RepB plasmid partitioning protein, at 8,572 to 9,186
_00009
H281DRAFT_00010: H281DRAFT_00010 - nitrile hydratase, at 9,487 to 10,146
_00010
H281DRAFT_00011: H281DRAFT_00011 - nitrile hydratase, at 10,143 to 10,814
_00011
H281DRAFT_00012: H281DRAFT_00012 - nitrile hydratase accessory protein, at 10,816 to 11,184
_00012
H281DRAFT_00013: H281DRAFT_00013 - Cytochrome P450, at 11,769 to 13,196
_00013
Position (kb) 10
11 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 9.220 kb on + strand at 9.221 kb on - strand at 9.238 kb on + strand at 9.268 kb on + strand at 9.269 kb on - strand at 9.310 kb on - strand at 9.450 kb on + strand at 9.450 kb on + strand at 9.608 kb on - strand, within H281DRAFT_00010 at 9.647 kb on - strand, within H281DRAFT_00010 at 9.686 kb on + strand, within H281DRAFT_00010 at 9.713 kb on - strand, within H281DRAFT_00010 at 9.834 kb on + strand, within H281DRAFT_00010 at 9.935 kb on + strand, within H281DRAFT_00010 at 10.142 kb on + strand at 10.142 kb on + strand at 10.142 kb on + strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.143 kb on - strand at 10.330 kb on - strand, within H281DRAFT_00011 at 10.345 kb on - strand, within H281DRAFT_00011 at 10.347 kb on + strand, within H281DRAFT_00011 at 10.348 kb on - strand, within H281DRAFT_00011 at 10.444 kb on + strand, within H281DRAFT_00011 at 10.444 kb on + strand, within H281DRAFT_00011 at 10.602 kb on + strand, within H281DRAFT_00011 at 10.704 kb on - strand, within H281DRAFT_00011 at 10.704 kb on - strand, within H281DRAFT_00011 at 10.782 kb on + strand at 10.982 kb on - strand, within H281DRAFT_00012 at 11.041 kb on + strand, within H281DRAFT_00012 at 11.041 kb on + strand, within H281DRAFT_00012 at 11.042 kb on - strand, within H281DRAFT_00012 at 11.042 kb on - strand, within H281DRAFT_00012 at 11.045 kb on - strand, within H281DRAFT_00012 at 11.374 kb on + strand at 11.450 kb on - strand at 11.527 kb on - strand at 11.553 kb on + strand at 11.638 kb on + strand at 11.639 kb on - strand at 11.639 kb on - strand at 11.721 kb on + strand at 11.722 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x remove 9,220 + +0.7 9,221 - +0.1 9,238 + +0.0 9,268 + -2.5 9,269 - +0.9 9,310 - -0.4 9,450 + +1.0 9,450 + +0.3 9,608 - H281DRAFT_00010 0.18 +0.0 9,647 - H281DRAFT_00010 0.24 -2.2 9,686 + H281DRAFT_00010 0.30 +0.3 9,713 - H281DRAFT_00010 0.34 +1.1 9,834 + H281DRAFT_00010 0.53 +1.3 9,935 + H281DRAFT_00010 0.68 +0.4 10,142 + -0.2 10,142 + +1.0 10,142 + +0.5 10,143 - -0.4 10,143 - +1.0 10,143 - +1.4 10,143 - +0.4 10,143 - -0.6 10,143 - -0.5 10,143 - -3.5 10,330 - H281DRAFT_00011 0.28 +0.0 10,345 - H281DRAFT_00011 0.30 +0.7 10,347 + H281DRAFT_00011 0.30 -1.4 10,348 - H281DRAFT_00011 0.31 -0.2 10,444 + H281DRAFT_00011 0.45 -0.3 10,444 + H281DRAFT_00011 0.45 -0.8 10,602 + H281DRAFT_00011 0.68 -0.3 10,704 - H281DRAFT_00011 0.83 -1.1 10,704 - H281DRAFT_00011 0.83 +0.6 10,782 + +0.2 10,982 - H281DRAFT_00012 0.45 -0.5 11,041 + H281DRAFT_00012 0.61 +1.1 11,041 + H281DRAFT_00012 0.61 +0.2 11,042 - H281DRAFT_00012 0.61 -0.3 11,042 - H281DRAFT_00012 0.61 -0.1 11,045 - H281DRAFT_00012 0.62 +1.7 11,374 + -0.1 11,450 - +0.2 11,527 - -0.5 11,553 + +1.6 11,638 + +0.1 11,639 - +1.4 11,639 - -2.5 11,721 + -3.3 11,722 - -0.7
Or see this region's nucleotide sequence