Experiment: Recovery broth with Piperacillin 1e-05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13065 and BBR_RS13070 are separated by 244 nucleotides BBR_RS13070 and BBR_RS13075 are separated by 43 nucleotides BBR_RS13075 and BBR_RS13080 overlap by 8 nucleotides
BBR_RS13065: BBR_RS13065 - amino acid ABC transporter ATP-binding protein, at 704,076 to 704,906
_RS13065
BBR_RS13070: BBR_RS13070 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 705,151 to 707,043
_RS13070
BBR_RS13075: BBR_RS13075 - 23S rRNA pseudouridylate synthase, at 707,087 to 707,821
_RS13075
BBR_RS13080: BBR_RS13080 - LacI family DNA-binding transcriptional regulator, at 707,814 to 708,800
_RS13080
Position (kb)
705
706
707
708 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 704.157 kb on + strand at 704.165 kb on + strand, within BBR_RS13065 at 704.180 kb on - strand, within BBR_RS13065 at 704.192 kb on + strand, within BBR_RS13065 at 704.192 kb on + strand, within BBR_RS13065 at 704.200 kb on - strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.219 kb on + strand, within BBR_RS13065 at 704.232 kb on - strand, within BBR_RS13065 at 704.264 kb on + strand, within BBR_RS13065 at 704.271 kb on + strand, within BBR_RS13065 at 704.271 kb on + strand, within BBR_RS13065 at 704.373 kb on + strand, within BBR_RS13065 at 704.381 kb on - strand, within BBR_RS13065 at 704.383 kb on + strand, within BBR_RS13065 at 704.424 kb on + strand, within BBR_RS13065 at 704.430 kb on + strand, within BBR_RS13065 at 704.436 kb on - strand, within BBR_RS13065 at 704.504 kb on + strand, within BBR_RS13065 at 704.512 kb on - strand, within BBR_RS13065 at 704.518 kb on - strand, within BBR_RS13065 at 704.534 kb on + strand, within BBR_RS13065 at 704.562 kb on - strand, within BBR_RS13065 at 704.567 kb on - strand, within BBR_RS13065 at 704.576 kb on + strand, within BBR_RS13065 at 704.605 kb on + strand, within BBR_RS13065 at 704.709 kb on + strand, within BBR_RS13065 at 704.711 kb on + strand, within BBR_RS13065 at 704.716 kb on + strand, within BBR_RS13065 at 704.719 kb on - strand, within BBR_RS13065 at 704.719 kb on - strand, within BBR_RS13065 at 704.724 kb on - strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.741 kb on + strand, within BBR_RS13065 at 704.748 kb on + strand, within BBR_RS13065 at 704.750 kb on - strand, within BBR_RS13065 at 704.754 kb on + strand, within BBR_RS13065 at 704.784 kb on - strand, within BBR_RS13065 at 704.898 kb on + strand at 704.958 kb on + strand at 704.958 kb on + strand at 704.969 kb on + strand at 705.022 kb on + strand at 705.025 kb on + strand at 706.065 kb on + strand, within BBR_RS13070 at 707.058 kb on - strand at 707.098 kb on + strand at 707.098 kb on + strand at 707.098 kb on + strand at 707.108 kb on + strand at 707.123 kb on - strand at 707.188 kb on - strand, within BBR_RS13075 at 707.202 kb on + strand, within BBR_RS13075 at 707.202 kb on - strand, within BBR_RS13075 at 707.210 kb on - strand, within BBR_RS13075 at 707.225 kb on + strand, within BBR_RS13075 at 707.263 kb on + strand, within BBR_RS13075 at 707.266 kb on - strand, within BBR_RS13075 at 707.322 kb on - strand, within BBR_RS13075 at 707.407 kb on - strand, within BBR_RS13075 at 707.439 kb on + strand, within BBR_RS13075 at 707.538 kb on + strand, within BBR_RS13075 at 707.571 kb on - strand, within BBR_RS13075 at 707.573 kb on + strand, within BBR_RS13075 at 707.574 kb on + strand, within BBR_RS13075 at 707.582 kb on - strand, within BBR_RS13075 at 707.604 kb on + strand, within BBR_RS13075 at 707.653 kb on - strand, within BBR_RS13075 at 707.655 kb on - strand, within BBR_RS13075 at 707.660 kb on + strand, within BBR_RS13075 at 707.668 kb on - strand, within BBR_RS13075 at 707.668 kb on - strand, within BBR_RS13075 at 707.775 kb on - strand at 707.777 kb on - strand at 707.807 kb on + strand at 707.807 kb on - strand at 707.817 kb on + strand at 707.857 kb on - strand at 707.893 kb on - strand at 707.919 kb on - strand, within BBR_RS13080 at 707.919 kb on - strand, within BBR_RS13080 at 707.957 kb on + strand, within BBR_RS13080 at 707.983 kb on + strand, within BBR_RS13080
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Piperacillin 1e-05 mM remove 704,157 + -0.0 704,165 + BBR_RS13065 0.11 +0.4 704,180 - BBR_RS13065 0.13 -0.7 704,192 + BBR_RS13065 0.14 -1.1 704,192 + BBR_RS13065 0.14 -0.2 704,200 - BBR_RS13065 0.15 +0.7 704,219 + BBR_RS13065 0.17 -0.6 704,219 + BBR_RS13065 0.17 +0.9 704,219 + BBR_RS13065 0.17 +0.2 704,232 - BBR_RS13065 0.19 -0.2 704,264 + BBR_RS13065 0.23 -0.4 704,271 + BBR_RS13065 0.23 +0.6 704,271 + BBR_RS13065 0.23 +0.3 704,373 + BBR_RS13065 0.36 -0.2 704,381 - BBR_RS13065 0.37 -0.9 704,383 + BBR_RS13065 0.37 +0.8 704,424 + BBR_RS13065 0.42 -1.0 704,430 + BBR_RS13065 0.43 +0.0 704,436 - BBR_RS13065 0.43 +0.2 704,504 + BBR_RS13065 0.52 +0.1 704,512 - BBR_RS13065 0.52 +0.4 704,518 - BBR_RS13065 0.53 +0.0 704,534 + BBR_RS13065 0.55 -0.6 704,562 - BBR_RS13065 0.58 +0.2 704,567 - BBR_RS13065 0.59 +0.4 704,576 + BBR_RS13065 0.60 +0.1 704,605 + BBR_RS13065 0.64 +0.0 704,709 + BBR_RS13065 0.76 -1.1 704,711 + BBR_RS13065 0.76 -0.5 704,716 + BBR_RS13065 0.77 -1.6 704,719 - BBR_RS13065 0.77 +0.6 704,719 - BBR_RS13065 0.77 -0.0 704,724 - BBR_RS13065 0.78 +0.4 704,741 + BBR_RS13065 0.80 +0.5 704,741 + BBR_RS13065 0.80 +0.0 704,741 + BBR_RS13065 0.80 +0.1 704,748 + BBR_RS13065 0.81 +0.6 704,750 - BBR_RS13065 0.81 +0.3 704,754 + BBR_RS13065 0.82 +1.7 704,784 - BBR_RS13065 0.85 +1.2 704,898 + -0.3 704,958 + -1.8 704,958 + -0.3 704,969 + +0.1 705,022 + -0.4 705,025 + -0.6 706,065 + BBR_RS13070 0.48 -1.8 707,058 - +1.3 707,098 + -0.3 707,098 + +0.5 707,098 + -0.5 707,108 + -0.6 707,123 - +0.6 707,188 - BBR_RS13075 0.14 -0.6 707,202 + BBR_RS13075 0.16 +0.3 707,202 - BBR_RS13075 0.16 -0.0 707,210 - BBR_RS13075 0.17 +2.3 707,225 + BBR_RS13075 0.19 +0.6 707,263 + BBR_RS13075 0.24 -0.6 707,266 - BBR_RS13075 0.24 +0.4 707,322 - BBR_RS13075 0.32 +0.3 707,407 - BBR_RS13075 0.44 +0.3 707,439 + BBR_RS13075 0.48 -0.8 707,538 + BBR_RS13075 0.61 +1.1 707,571 - BBR_RS13075 0.66 -2.0 707,573 + BBR_RS13075 0.66 +0.9 707,574 + BBR_RS13075 0.66 +0.3 707,582 - BBR_RS13075 0.67 +0.7 707,604 + BBR_RS13075 0.70 +0.1 707,653 - BBR_RS13075 0.77 -0.3 707,655 - BBR_RS13075 0.77 -0.2 707,660 + BBR_RS13075 0.78 -0.6 707,668 - BBR_RS13075 0.79 -1.0 707,668 - BBR_RS13075 0.79 -0.2 707,775 - +0.5 707,777 - +0.4 707,807 + -0.3 707,807 - +0.3 707,817 + -0.2 707,857 - -0.4 707,893 - -0.3 707,919 - BBR_RS13080 0.11 -0.4 707,919 - BBR_RS13080 0.11 +0.7 707,957 + BBR_RS13080 0.14 +0.9 707,983 + BBR_RS13080 0.17 -1.8
Or see this region's nucleotide sequence