Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2775 and Shewana3_2776 are separated by 458 nucleotides Shewana3_2776 and Shewana3_2777 are separated by 98 nucleotides Shewana3_2777 and Shewana3_2778 are separated by 84 nucleotides
Shewana3_2775: Shewana3_2775 - outer membrane protein W (RefSeq), at 3,314,270 to 3,314,926
_2775
Shewana3_2776: Shewana3_2776 - hypothetical protein (RefSeq), at 3,315,385 to 3,315,972
_2776
Shewana3_2777: Shewana3_2777 - maleylacetoacetate isomerase (RefSeq), at 3,316,071 to 3,316,721
_2777
Shewana3_2778: Shewana3_2778 - fumarylacetoacetate (FAA) hydrolase (RefSeq), at 3,316,806 to 3,317,792
_2778
Position (kb)
3315
3316 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3314.447 kb on + strand, within Shewana3_2775 at 3314.447 kb on + strand, within Shewana3_2775 at 3314.465 kb on - strand, within Shewana3_2775 at 3314.600 kb on + strand, within Shewana3_2775 at 3314.600 kb on + strand, within Shewana3_2775 at 3314.659 kb on - strand, within Shewana3_2775 at 3315.074 kb on + strand at 3315.144 kb on + strand at 3315.345 kb on + strand at 3315.374 kb on + strand at 3315.796 kb on + strand, within Shewana3_2776 at 3315.798 kb on + strand, within Shewana3_2776 at 3315.806 kb on - strand, within Shewana3_2776 at 3315.806 kb on - strand, within Shewana3_2776 at 3315.913 kb on - strand, within Shewana3_2776 at 3315.924 kb on - strand at 3315.929 kb on - strand at 3315.946 kb on - strand at 3316.068 kb on + strand at 3316.135 kb on + strand at 3316.135 kb on + strand at 3316.200 kb on - strand, within Shewana3_2777 at 3316.324 kb on + strand, within Shewana3_2777 at 3316.357 kb on + strand, within Shewana3_2777 at 3316.517 kb on - strand, within Shewana3_2777 at 3316.732 kb on + strand at 3316.803 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 3,314,447 + Shewana3_2775 0.27 +0.2 3,314,447 + Shewana3_2775 0.27 -0.1 3,314,465 - Shewana3_2775 0.30 +0.1 3,314,600 + Shewana3_2775 0.50 +0.6 3,314,600 + Shewana3_2775 0.50 +0.2 3,314,659 - Shewana3_2775 0.59 +0.3 3,315,074 + -0.7 3,315,144 + -0.4 3,315,345 + +2.8 3,315,374 + +1.0 3,315,796 + Shewana3_2776 0.70 -0.8 3,315,798 + Shewana3_2776 0.70 +0.2 3,315,806 - Shewana3_2776 0.72 -0.8 3,315,806 - Shewana3_2776 0.72 -1.1 3,315,913 - Shewana3_2776 0.90 +0.0 3,315,924 - -0.5 3,315,929 - +0.2 3,315,946 - -1.3 3,316,068 + +1.2 3,316,135 + -1.5 3,316,135 + +0.2 3,316,200 - Shewana3_2777 0.20 -2.1 3,316,324 + Shewana3_2777 0.39 -0.1 3,316,357 + Shewana3_2777 0.44 +0.7 3,316,517 - Shewana3_2777 0.69 +0.4 3,316,732 + +0.1 3,316,803 + +0.6
Or see this region's nucleotide sequence