Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2545 and purN overlap by 1 nucleotides purN and Shewana3_2547 are separated by 138 nucleotides Shewana3_2547 and Shewana3_2548 are separated by 197 nucleotides
Shewana3_2545: Shewana3_2545 - phosphoribosylaminoimidazole synthetase (RefSeq), at 3,042,708 to 3,043,745
_2545
Shewana3_2546: purN - phosphoribosylglycinamide formyltransferase (RefSeq), at 3,043,745 to 3,044,389
purN
Shewana3_2547: Shewana3_2547 - UMP phosphatase (RefSeq), at 3,044,528 to 3,045,274
_2547
Shewana3_2548: Shewana3_2548 - peptidase M28 (RefSeq), at 3,045,472 to 3,047,145
_2548
Position (kb)
3044
3045
3046 Strain fitness (log2 ratio)
-2
-1
0
1 at 3043.696 kb on + strand at 3043.725 kb on + strand at 3043.756 kb on - strand at 3043.758 kb on + strand at 3043.826 kb on + strand, within purN at 3044.089 kb on + strand, within purN at 3044.172 kb on + strand, within purN at 3044.278 kb on + strand, within purN at 3044.307 kb on + strand, within purN at 3044.315 kb on - strand, within purN at 3044.675 kb on - strand, within Shewana3_2547 at 3044.816 kb on - strand, within Shewana3_2547 at 3045.074 kb on + strand, within Shewana3_2547 at 3045.172 kb on - strand, within Shewana3_2547 at 3045.300 kb on + strand at 3045.465 kb on - strand at 3045.643 kb on - strand, within Shewana3_2548 at 3046.148 kb on - strand, within Shewana3_2548
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 3,043,696 + +0.4 3,043,725 + +0.7 3,043,756 - -0.9 3,043,758 + +0.4 3,043,826 + purN Shewana3_2546 0.13 +1.0 3,044,089 + purN Shewana3_2546 0.53 +1.4 3,044,172 + purN Shewana3_2546 0.66 -0.1 3,044,278 + purN Shewana3_2546 0.83 +0.3 3,044,307 + purN Shewana3_2546 0.87 +0.7 3,044,315 - purN Shewana3_2546 0.88 +0.7 3,044,675 - Shewana3_2547 0.20 -0.5 3,044,816 - Shewana3_2547 0.39 -2.4 3,045,074 + Shewana3_2547 0.73 -1.2 3,045,172 - Shewana3_2547 0.86 +0.7 3,045,300 + +1.3 3,045,465 - +1.3 3,045,643 - Shewana3_2548 0.10 -1.4 3,046,148 - Shewana3_2548 0.40 +0.0
Or see this region's nucleotide sequence