Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2172 and Shewana3_2173 overlap by 8 nucleotides Shewana3_2173 and Shewana3_2174 are separated by 66 nucleotides Shewana3_2174 and Shewana3_2175 are separated by 113 nucleotides
Shewana3_2172: Shewana3_2172 - electron transport complex protein RnfG (RefSeq), at 2,605,396 to 2,606,022
_2172
Shewana3_2173: Shewana3_2173 - electron transport complex RsxE subunit (RefSeq), at 2,606,015 to 2,606,713
_2173
Shewana3_2174: Shewana3_2174 - DNA-(apurinic or apyrimidinic site) lyase / endonuclease III (RefSeq), at 2,606,780 to 2,607,415
_2174
Shewana3_2175: Shewana3_2175 - phage integrase family protein (RefSeq), at 2,607,529 to 2,608,851
_2175
Position (kb)
2606
2607
2608 Strain fitness (log2 ratio)
-1
0
1
2 at 2606.641 kb on + strand, within Shewana3_2173 at 2606.950 kb on + strand, within Shewana3_2174 at 2606.952 kb on + strand, within Shewana3_2174 at 2607.097 kb on + strand, within Shewana3_2174 at 2607.486 kb on - strand at 2607.621 kb on + strand at 2607.982 kb on - strand, within Shewana3_2175 at 2608.280 kb on + strand, within Shewana3_2175
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 2,606,641 + Shewana3_2173 0.90 -0.2 2,606,950 + Shewana3_2174 0.27 -0.2 2,606,952 + Shewana3_2174 0.27 +2.4 2,607,097 + Shewana3_2174 0.50 -0.2 2,607,486 - +1.4 2,607,621 + +2.0 2,607,982 - Shewana3_2175 0.34 +2.4 2,608,280 + Shewana3_2175 0.57 +2.4
Or see this region's nucleotide sequence