Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1847 and Shewana3_1848 are separated by 44 nucleotides Shewana3_1848 and Shewana3_1849 overlap by 19 nucleotides Shewana3_1849 and hisH are separated by 29 nucleotides
Shewana3_1847: Shewana3_1847 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,189,352 to 2,190,011
_1847
Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829
_1848
Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548
_1849
Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228
hisH
Position (kb)
2190
2191 Strain fitness (log2 ratio)
-1
0
1
2 at 2189.292 kb on + strand at 2189.292 kb on + strand at 2189.332 kb on - strand at 2189.337 kb on - strand at 2189.337 kb on - strand at 2189.339 kb on - strand at 2189.575 kb on + strand, within Shewana3_1847 at 2189.640 kb on + strand, within Shewana3_1847 at 2189.642 kb on + strand, within Shewana3_1847 at 2189.996 kb on - strand at 2190.055 kb on - strand at 2190.406 kb on + strand, within Shewana3_1848 at 2190.633 kb on + strand, within Shewana3_1848 at 2190.634 kb on + strand, within Shewana3_1848 at 2190.641 kb on - strand, within Shewana3_1848 at 2190.778 kb on - strand at 2190.826 kb on + strand at 2190.981 kb on + strand, within Shewana3_1849 at 2190.989 kb on - strand, within Shewana3_1849 at 2191.094 kb on - strand, within Shewana3_1849 at 2191.651 kb on + strand, within hisH at 2191.714 kb on - strand, within hisH
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 2,189,292 + +0.4 2,189,292 + +0.9 2,189,332 - +0.2 2,189,337 - +1.8 2,189,337 - -0.8 2,189,339 - -0.9 2,189,575 + Shewana3_1847 0.34 +0.8 2,189,640 + Shewana3_1847 0.44 +0.1 2,189,642 + Shewana3_1847 0.44 +0.8 2,189,996 - -0.7 2,190,055 - +1.4 2,190,406 + Shewana3_1848 0.45 -1.1 2,190,633 + Shewana3_1848 0.75 -1.0 2,190,634 + Shewana3_1848 0.75 -0.5 2,190,641 - Shewana3_1848 0.76 -1.2 2,190,778 - +0.2 2,190,826 + +1.5 2,190,981 + Shewana3_1849 0.23 +0.9 2,190,989 - Shewana3_1849 0.24 -0.3 2,191,094 - Shewana3_1849 0.38 +0.8 2,191,651 + hisH Shewana3_1850 0.11 +2.5 2,191,714 - hisH Shewana3_1850 0.21 +0.0
Or see this region's nucleotide sequence