Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1120 and surE overlap by 4 nucleotides surE and Shewana3_1122 overlap by 4 nucleotides Shewana3_1122 and Shewana3_1123 are separated by 84 nucleotides
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Shewana3_1123: Shewana3_1123 - peptidase M23B (RefSeq), at 1,321,357 to 1,322,253
_1123
Position (kb)
1320
1321
1322 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1319.876 kb on + strand at 1319.886 kb on + strand at 1319.978 kb on + strand, within surE at 1320.132 kb on - strand, within surE at 1320.232 kb on + strand, within surE at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122 at 1320.950 kb on + strand, within Shewana3_1122 at 1321.345 kb on - strand at 1321.439 kb on - strand at 1321.544 kb on - strand, within Shewana3_1123 at 1321.878 kb on - strand, within Shewana3_1123 at 1321.922 kb on + strand, within Shewana3_1123 at 1322.101 kb on + strand, within Shewana3_1123 at 1322.243 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 1,319,876 + -0.1 1,319,886 + -0.6 1,319,978 + surE Shewana3_1121 0.12 -0.0 1,320,132 - surE Shewana3_1121 0.32 -0.8 1,320,232 + surE Shewana3_1121 0.45 +0.5 1,320,397 + surE Shewana3_1121 0.67 +0.7 1,320,433 + surE Shewana3_1121 0.72 +0.1 1,320,441 - surE Shewana3_1121 0.73 -0.9 1,320,578 + +0.2 1,320,728 + Shewana3_1122 0.14 -0.9 1,320,950 + Shewana3_1122 0.49 -0.5 1,321,345 - -1.8 1,321,439 - +0.5 1,321,544 - Shewana3_1123 0.21 +2.1 1,321,878 - Shewana3_1123 0.58 -0.9 1,321,922 + Shewana3_1123 0.63 -0.5 1,322,101 + Shewana3_1123 0.83 -0.3 1,322,243 - +2.1
Or see this region's nucleotide sequence