Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shewana3_0709 are separated by 70 nucleotides Shewana3_0709 and Shewana3_0710 are separated by 13 nucleotides Shewana3_0710 and Shewana3_0711 are separated by 12 nucleotides
Shewana3_0708: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 822,821 to 824,260
thiH
Shewana3_0709: Shewana3_0709 - formate dehydrogenase gamma subunit (RefSeq), at 824,331 to 825,014
_0709
Shewana3_0710: Shewana3_0710 - iron hydrogenase, small subunit (RefSeq), at 825,028 to 825,348
_0710
Shewana3_0711: Shewana3_0711 - response regulator receiver protein (RefSeq), at 825,361 to 826,593
_0711
Position (kb) 825
826 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 824.096 kb on + strand, within thiH at 824.104 kb on - strand, within thiH at 824.454 kb on - strand, within Shewana3_0709 at 824.581 kb on - strand, within Shewana3_0709 at 824.726 kb on + strand, within Shewana3_0709 at 824.798 kb on - strand, within Shewana3_0709 at 824.855 kb on + strand, within Shewana3_0709 at 824.855 kb on + strand, within Shewana3_0709 at 824.863 kb on - strand, within Shewana3_0709 at 825.111 kb on - strand, within Shewana3_0710 at 825.253 kb on + strand, within Shewana3_0710 at 825.436 kb on + strand at 825.444 kb on - strand at 825.452 kb on - strand at 825.489 kb on + strand, within Shewana3_0711 at 825.525 kb on - strand, within Shewana3_0711 at 825.560 kb on + strand, within Shewana3_0711 at 825.920 kb on + strand, within Shewana3_0711 at 826.047 kb on + strand, within Shewana3_0711 at 826.047 kb on - strand, within Shewana3_0711 at 826.085 kb on + strand, within Shewana3_0711 at 826.224 kb on - strand, within Shewana3_0711 at 826.249 kb on - strand, within Shewana3_0711 at 826.249 kb on - strand, within Shewana3_0711 at 826.263 kb on - strand, within Shewana3_0711 at 826.277 kb on + strand, within Shewana3_0711 at 826.332 kb on + strand, within Shewana3_0711
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 824,096 + thiH Shewana3_0708 0.89 +0.1 824,104 - thiH Shewana3_0708 0.89 +0.6 824,454 - Shewana3_0709 0.18 +0.2 824,581 - Shewana3_0709 0.37 -0.1 824,726 + Shewana3_0709 0.58 -1.8 824,798 - Shewana3_0709 0.68 -0.4 824,855 + Shewana3_0709 0.77 -0.6 824,855 + Shewana3_0709 0.77 -0.1 824,863 - Shewana3_0709 0.78 +1.9 825,111 - Shewana3_0710 0.26 +0.5 825,253 + Shewana3_0710 0.70 -4.0 825,436 + +0.4 825,444 - +0.4 825,452 - +0.2 825,489 + Shewana3_0711 0.10 -1.4 825,525 - Shewana3_0711 0.13 +0.2 825,560 + Shewana3_0711 0.16 -1.5 825,920 + Shewana3_0711 0.45 -0.1 826,047 + Shewana3_0711 0.56 -0.1 826,047 - Shewana3_0711 0.56 -1.9 826,085 + Shewana3_0711 0.59 -0.6 826,224 - Shewana3_0711 0.70 -1.3 826,249 - Shewana3_0711 0.72 +0.2 826,249 - Shewana3_0711 0.72 -0.5 826,263 - Shewana3_0711 0.73 -1.6 826,277 + Shewana3_0711 0.74 -0.2 826,332 + Shewana3_0711 0.79 -0.4
Or see this region's nucleotide sequence