Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0584 and Shewana3_0585 are separated by 23 nucleotides Shewana3_0585 and Shewana3_0586 are separated by 15 nucleotides Shewana3_0586 and Shewana3_0587 are separated by 68 nucleotides Shewana3_0587 and Shewana3_0588 are separated by 14 nucleotides
Shewana3_0584: Shewana3_0584 - hypothetical protein (RefSeq), at 684,630 to 684,965
_0584
Shewana3_0585: Shewana3_0585 - glycerophosphoryl diester phosphodiesterase (RefSeq), at 684,989 to 685,711
_0585
Shewana3_0586: Shewana3_0586 - MscS mechanosensitive ion channel (RefSeq), at 685,727 to 688,930
_0586
Shewana3_0587: Shewana3_0587 - hypothetical protein (RefSeq), at 688,999 to 689,883
_0587
Shewana3_0588: Shewana3_0588 - phosphatidylserine decarboxylase (RefSeq), at 689,898 to 690,776
_0588
Position (kb) 685
686
687
688
689 Strain fitness (log2 ratio) -2
-1
0
1
2 at 684.772 kb on - strand, within Shewana3_0584 at 684.772 kb on - strand, within Shewana3_0584 at 684.789 kb on - strand, within Shewana3_0584 at 684.813 kb on + strand, within Shewana3_0584 at 684.968 kb on - strand at 685.085 kb on - strand, within Shewana3_0585 at 685.087 kb on + strand, within Shewana3_0585 at 685.093 kb on - strand, within Shewana3_0585 at 685.130 kb on - strand, within Shewana3_0585 at 685.143 kb on + strand, within Shewana3_0585 at 685.201 kb on + strand, within Shewana3_0585 at 685.401 kb on - strand, within Shewana3_0585 at 685.606 kb on - strand, within Shewana3_0585 at 685.628 kb on + strand, within Shewana3_0585 at 685.649 kb on - strand at 685.879 kb on + strand at 685.889 kb on + strand at 685.950 kb on + strand at 685.983 kb on + strand at 686.167 kb on - strand, within Shewana3_0586 at 686.323 kb on + strand, within Shewana3_0586 at 686.375 kb on - strand, within Shewana3_0586 at 686.430 kb on - strand, within Shewana3_0586 at 686.564 kb on - strand, within Shewana3_0586 at 686.793 kb on - strand, within Shewana3_0586 at 686.908 kb on - strand, within Shewana3_0586 at 686.949 kb on + strand, within Shewana3_0586 at 686.951 kb on + strand, within Shewana3_0586 at 686.962 kb on + strand, within Shewana3_0586 at 686.965 kb on - strand, within Shewana3_0586 at 686.970 kb on - strand, within Shewana3_0586 at 686.977 kb on - strand, within Shewana3_0586 at 687.092 kb on + strand, within Shewana3_0586 at 687.100 kb on - strand, within Shewana3_0586 at 687.156 kb on - strand, within Shewana3_0586 at 687.232 kb on - strand, within Shewana3_0586 at 687.270 kb on + strand, within Shewana3_0586 at 687.435 kb on - strand, within Shewana3_0586 at 687.435 kb on - strand, within Shewana3_0586 at 687.441 kb on - strand, within Shewana3_0586 at 687.441 kb on - strand, within Shewana3_0586 at 687.462 kb on - strand, within Shewana3_0586 at 687.500 kb on + strand, within Shewana3_0586 at 687.553 kb on + strand, within Shewana3_0586 at 687.566 kb on - strand, within Shewana3_0586 at 687.722 kb on - strand, within Shewana3_0586 at 687.749 kb on + strand, within Shewana3_0586 at 687.757 kb on - strand, within Shewana3_0586 at 687.758 kb on + strand, within Shewana3_0586 at 687.850 kb on + strand, within Shewana3_0586 at 687.858 kb on - strand, within Shewana3_0586 at 687.906 kb on - strand, within Shewana3_0586 at 687.906 kb on - strand, within Shewana3_0586 at 687.944 kb on - strand, within Shewana3_0586 at 687.948 kb on - strand, within Shewana3_0586 at 688.327 kb on - strand, within Shewana3_0586 at 688.749 kb on - strand at 689.200 kb on + strand, within Shewana3_0587 at 689.200 kb on + strand, within Shewana3_0587 at 689.252 kb on - strand, within Shewana3_0587 at 689.431 kb on + strand, within Shewana3_0587 at 689.439 kb on - strand, within Shewana3_0587 at 689.462 kb on - strand, within Shewana3_0587 at 689.462 kb on - strand, within Shewana3_0587 at 689.640 kb on + strand, within Shewana3_0587 at 689.640 kb on + strand, within Shewana3_0587 at 689.658 kb on + strand, within Shewana3_0587 at 689.740 kb on + strand, within Shewana3_0587 at 689.748 kb on - strand, within Shewana3_0587 at 689.784 kb on + strand, within Shewana3_0587 at 689.837 kb on + strand at 689.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 684,772 - Shewana3_0584 0.42 -0.7 684,772 - Shewana3_0584 0.42 -0.1 684,789 - Shewana3_0584 0.47 -0.4 684,813 + Shewana3_0584 0.54 +0.6 684,968 - -1.3 685,085 - Shewana3_0585 0.13 +1.0 685,087 + Shewana3_0585 0.14 -1.1 685,093 - Shewana3_0585 0.14 -0.2 685,130 - Shewana3_0585 0.20 -0.2 685,143 + Shewana3_0585 0.21 -0.6 685,201 + Shewana3_0585 0.29 -0.4 685,401 - Shewana3_0585 0.57 -1.4 685,606 - Shewana3_0585 0.85 -0.5 685,628 + Shewana3_0585 0.88 -1.1 685,649 - -0.6 685,879 + -1.9 685,889 + -2.2 685,950 + +0.3 685,983 + -2.3 686,167 - Shewana3_0586 0.14 -1.0 686,323 + Shewana3_0586 0.19 +0.0 686,375 - Shewana3_0586 0.20 +1.5 686,430 - Shewana3_0586 0.22 -0.7 686,564 - Shewana3_0586 0.26 -0.4 686,793 - Shewana3_0586 0.33 -2.1 686,908 - Shewana3_0586 0.37 -0.5 686,949 + Shewana3_0586 0.38 -0.8 686,951 + Shewana3_0586 0.38 -0.6 686,962 + Shewana3_0586 0.39 -0.9 686,965 - Shewana3_0586 0.39 -1.9 686,970 - Shewana3_0586 0.39 +0.2 686,977 - Shewana3_0586 0.39 -0.6 687,092 + Shewana3_0586 0.43 -1.9 687,100 - Shewana3_0586 0.43 -2.4 687,156 - Shewana3_0586 0.45 -2.2 687,232 - Shewana3_0586 0.47 -2.4 687,270 + Shewana3_0586 0.48 -0.7 687,435 - Shewana3_0586 0.53 -1.6 687,435 - Shewana3_0586 0.53 +1.2 687,441 - Shewana3_0586 0.53 -0.7 687,441 - Shewana3_0586 0.53 -0.4 687,462 - Shewana3_0586 0.54 -0.6 687,500 + Shewana3_0586 0.55 -0.2 687,553 + Shewana3_0586 0.57 +0.1 687,566 - Shewana3_0586 0.57 -0.4 687,722 - Shewana3_0586 0.62 -0.3 687,749 + Shewana3_0586 0.63 -0.2 687,757 - Shewana3_0586 0.63 +0.2 687,758 + Shewana3_0586 0.63 -0.4 687,850 + Shewana3_0586 0.66 -0.8 687,858 - Shewana3_0586 0.67 -0.7 687,906 - Shewana3_0586 0.68 -2.0 687,906 - Shewana3_0586 0.68 -0.9 687,944 - Shewana3_0586 0.69 -1.0 687,948 - Shewana3_0586 0.69 -0.3 688,327 - Shewana3_0586 0.81 -0.6 688,749 - -1.3 689,200 + Shewana3_0587 0.23 -1.6 689,200 + Shewana3_0587 0.23 -0.3 689,252 - Shewana3_0587 0.29 -1.8 689,431 + Shewana3_0587 0.49 +1.8 689,439 - Shewana3_0587 0.50 -0.4 689,462 - Shewana3_0587 0.52 -0.7 689,462 - Shewana3_0587 0.52 -0.3 689,640 + Shewana3_0587 0.72 -1.4 689,640 + Shewana3_0587 0.72 -1.0 689,658 + Shewana3_0587 0.74 -0.8 689,740 + Shewana3_0587 0.84 +0.0 689,748 - Shewana3_0587 0.85 -0.8 689,784 + Shewana3_0587 0.89 +0.1 689,837 + -0.6 689,865 + +1.9
Or see this region's nucleotide sequence