Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0415 and coaE overlap by 8 nucleotides coaE and Shewana3_0417 are separated by 93 nucleotides Shewana3_0417 and Shewana3_0418 are separated by 61 nucleotides
Shewana3_0415: Shewana3_0415 - hypothetical protein (RefSeq), at 473,002 to 473,736
_0415
Shewana3_0416: coaE - dephospho-CoA kinase (RefSeq), at 473,729 to 474,346
coaE
Shewana3_0417: Shewana3_0417 - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A (RefSeq), at 474,440 to 475,369
_0417
Shewana3_0418: Shewana3_0418 - type II secretion system protein (RefSeq), at 475,431 to 476,702
_0418
Position (kb) 474
475
476 Strain fitness (log2 ratio) -2
-1
0
1 at 473.651 kb on + strand, within Shewana3_0415 at 473.705 kb on + strand at 473.715 kb on + strand at 474.637 kb on - strand, within Shewana3_0417 at 474.999 kb on + strand, within Shewana3_0417 at 475.035 kb on - strand, within Shewana3_0417 at 475.133 kb on - strand, within Shewana3_0417 at 475.340 kb on + strand at 475.348 kb on - strand at 475.427 kb on - strand at 475.791 kb on + strand, within Shewana3_0418 at 476.070 kb on + strand, within Shewana3_0418 at 476.091 kb on + strand, within Shewana3_0418 at 476.202 kb on + strand, within Shewana3_0418 at 476.224 kb on + strand, within Shewana3_0418 at 476.229 kb on + strand, within Shewana3_0418
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 473,651 + Shewana3_0415 0.88 -0.7 473,705 + +0.0 473,715 + +0.3 474,637 - Shewana3_0417 0.21 -2.6 474,999 + Shewana3_0417 0.60 +0.0 475,035 - Shewana3_0417 0.64 -1.3 475,133 - Shewana3_0417 0.75 -1.1 475,340 + -1.2 475,348 - -1.0 475,427 - -0.6 475,791 + Shewana3_0418 0.28 -1.9 476,070 + Shewana3_0418 0.50 -0.0 476,091 + Shewana3_0418 0.52 -1.5 476,202 + Shewana3_0418 0.61 +0.9 476,224 + Shewana3_0418 0.62 +1.0 476,229 + Shewana3_0418 0.63 -0.6
Or see this region's nucleotide sequence