Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0411 and Shewana3_0412 are separated by 74 nucleotides Shewana3_0412 and Shewana3_0413 are separated by 147 nucleotides Shewana3_0413 and Shewana3_0414 are separated by 34 nucleotides Shewana3_0414 and Shewana3_0415 are separated by 107 nucleotides
Shewana3_0411: Shewana3_0411 - Ppx/GppA phosphatase (RefSeq), at 470,567 to 471,493
_0411
Shewana3_0412: Shewana3_0412 - hypothetical protein (RefSeq), at 471,568 to 472,104
_0412
Shewana3_0413: Shewana3_0413 - mutator MutT protein (RefSeq), at 472,252 to 472,650
_0413
Shewana3_0414: Shewana3_0414 - hypothetical protein (RefSeq), at 472,685 to 472,894
_0414
Shewana3_0415: Shewana3_0415 - hypothetical protein (RefSeq), at 473,002 to 473,736
_0415
Position (kb) 472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 471.261 kb on + strand, within Shewana3_0411 at 471.280 kb on + strand, within Shewana3_0411 at 471.280 kb on + strand, within Shewana3_0411 at 471.528 kb on + strand at 471.529 kb on + strand at 471.553 kb on - strand at 471.725 kb on + strand, within Shewana3_0412 at 471.969 kb on + strand, within Shewana3_0412 at 472.172 kb on - strand at 472.445 kb on - strand, within Shewana3_0413 at 472.503 kb on - strand, within Shewana3_0413 at 472.622 kb on - strand at 472.710 kb on + strand, within Shewana3_0414 at 472.744 kb on + strand, within Shewana3_0414 at 472.984 kb on - strand at 472.991 kb on - strand at 473.042 kb on + strand at 473.072 kb on - strand at 473.219 kb on - strand, within Shewana3_0415
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 471,261 + Shewana3_0411 0.75 +0.4 471,280 + Shewana3_0411 0.77 +0.1 471,280 + Shewana3_0411 0.77 +0.1 471,528 + -1.2 471,529 + -0.2 471,553 - -0.3 471,725 + Shewana3_0412 0.29 +2.0 471,969 + Shewana3_0412 0.75 -0.2 472,172 - -0.2 472,445 - Shewana3_0413 0.48 -0.4 472,503 - Shewana3_0413 0.63 -0.1 472,622 - -0.5 472,710 + Shewana3_0414 0.12 -1.0 472,744 + Shewana3_0414 0.28 -2.0 472,984 - -0.6 472,991 - -2.0 473,042 + -1.9 473,072 - -1.1 473,219 - Shewana3_0415 0.30 -2.8
Or see this region's nucleotide sequence