Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0224 and rplQ are separated by 40 nucleotides rplQ and Shewana3_0226 are separated by 65 nucleotides Shewana3_0226 and Shewana3_0227 are separated by 114 nucleotides Shewana3_0227 and Shewana3_0228 overlap by 4 nucleotides Shewana3_0228 and Shewana3_0229 overlap by 1 nucleotides
Shewana3_0224: Shewana3_0224 - DNA-directed RNA polymerase subunit alpha (RefSeq), at 255,491 to 256,480
_0224
Shewana3_0225: rplQ - 50S ribosomal protein L17 (RefSeq), at 256,521 to 256,916
rplQ
Shewana3_0226: Shewana3_0226 - hypothetical protein (RefSeq), at 256,982 to 257,986
_0226
Shewana3_0227: Shewana3_0227 - cytochrome c-type biogenesis protein CcmE (RefSeq), at 258,101 to 258,586
_0227
Shewana3_0228: Shewana3_0228 - Heme exporter protein D (CcmD) (RefSeq), at 258,583 to 258,783
_0228
Shewana3_0229: Shewana3_0229 - heme exporter protein CcmC (RefSeq), at 258,783 to 259,529
_0229
Position (kb) 256
257
258 Strain fitness (log2 ratio) -1
0
1
2
3 at 257.337 kb on + strand, within Shewana3_0226 at 257.362 kb on + strand, within Shewana3_0226 at 257.485 kb on - strand, within Shewana3_0226 at 257.757 kb on + strand, within Shewana3_0226 at 257.904 kb on + strand at 257.990 kb on - strand at 258.015 kb on + strand at 258.020 kb on + strand at 258.373 kb on + strand, within Shewana3_0227 at 258.381 kb on - strand, within Shewana3_0227 at 258.381 kb on - strand, within Shewana3_0227 at 258.444 kb on + strand, within Shewana3_0227 at 258.654 kb on - strand, within Shewana3_0228 at 258.737 kb on - strand, within Shewana3_0228 at 258.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 257,337 + Shewana3_0226 0.35 -1.4 257,362 + Shewana3_0226 0.38 -0.2 257,485 - Shewana3_0226 0.50 -1.1 257,757 + Shewana3_0226 0.77 -1.0 257,904 + -0.1 257,990 - -1.0 258,015 + +0.2 258,020 + +0.2 258,373 + Shewana3_0227 0.56 +3.0 258,381 - Shewana3_0227 0.58 +2.5 258,381 - Shewana3_0227 0.58 +2.0 258,444 + Shewana3_0227 0.71 +0.2 258,654 - Shewana3_0228 0.35 +2.0 258,737 - Shewana3_0228 0.77 +3.2 258,807 - +0.6
Or see this region's nucleotide sequence