Experiment: Rifampicin 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0019 and Shewana3_0020 are separated by 181 nucleotides Shewana3_0020 and Shewana3_0021 are separated by 35 nucleotides Shewana3_0021 and Shewana3_0022 are separated by 152 nucleotides
Shewana3_0019: Shewana3_0019 - amidohydrolase (RefSeq), at 22,574 to 25,762
_0019
Shewana3_0020: Shewana3_0020 - MOSC domain-containing protein (RefSeq), at 25,944 to 26,381
_0020
Shewana3_0021: Shewana3_0021 - sulfur transfer protein SirA (RefSeq), at 26,417 to 26,662
_0021
Shewana3_0022: Shewana3_0022 - diguanylate cyclase (RefSeq), at 26,815 to 28,026
_0022
Position (kb) 25
26
27 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 24.985 kb on - strand, within Shewana3_0019 at 25.230 kb on - strand, within Shewana3_0019 at 25.333 kb on + strand, within Shewana3_0019 at 25.341 kb on - strand, within Shewana3_0019 at 25.501 kb on - strand at 25.686 kb on + strand at 25.699 kb on - strand at 25.763 kb on + strand at 25.854 kb on - strand at 25.896 kb on + strand at 25.912 kb on + strand at 25.919 kb on - strand at 25.929 kb on - strand at 25.955 kb on - strand at 25.955 kb on - strand at 26.087 kb on - strand, within Shewana3_0020 at 26.111 kb on - strand, within Shewana3_0020 at 26.250 kb on + strand, within Shewana3_0020 at 26.293 kb on + strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.301 kb on - strand, within Shewana3_0020 at 26.398 kb on - strand at 26.757 kb on + strand at 26.765 kb on - strand at 26.804 kb on + strand at 26.882 kb on + strand at 26.890 kb on - strand at 26.957 kb on - strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.167 kb on + strand, within Shewana3_0022 at 27.175 kb on - strand, within Shewana3_0022 at 27.242 kb on + strand, within Shewana3_0022 at 27.279 kb on - strand, within Shewana3_0022 at 27.314 kb on - strand, within Shewana3_0022 at 27.315 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.340 kb on + strand, within Shewana3_0022 at 27.348 kb on - strand, within Shewana3_0022 at 27.381 kb on + strand, within Shewana3_0022
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.001 mg/ml remove 24,985 - Shewana3_0019 0.76 -1.1 25,230 - Shewana3_0019 0.83 -2.4 25,333 + Shewana3_0019 0.87 -0.5 25,341 - Shewana3_0019 0.87 -0.2 25,501 - +0.1 25,686 + -0.5 25,699 - -0.1 25,763 + -0.1 25,854 - -0.4 25,896 + -0.7 25,912 + -0.8 25,919 - -2.5 25,929 - -0.4 25,955 - -0.1 25,955 - -0.7 26,087 - Shewana3_0020 0.33 -0.1 26,111 - Shewana3_0020 0.38 +0.0 26,250 + Shewana3_0020 0.70 -0.3 26,293 + Shewana3_0020 0.80 +6.4 26,301 - Shewana3_0020 0.82 +0.1 26,301 - Shewana3_0020 0.82 -0.4 26,301 - Shewana3_0020 0.82 -0.2 26,398 - -0.5 26,757 + -0.4 26,765 - -1.0 26,804 + -0.7 26,882 + +0.2 26,890 - -0.9 26,957 - Shewana3_0022 0.12 -2.5 27,167 + Shewana3_0022 0.29 +0.1 27,167 + Shewana3_0022 0.29 -0.0 27,175 - Shewana3_0022 0.30 -0.6 27,242 + Shewana3_0022 0.35 +1.7 27,279 - Shewana3_0022 0.38 -1.0 27,314 - Shewana3_0022 0.41 -1.2 27,315 + Shewana3_0022 0.41 +0.8 27,340 + Shewana3_0022 0.43 -0.5 27,340 + Shewana3_0022 0.43 -0.4 27,340 + Shewana3_0022 0.43 -0.0 27,348 - Shewana3_0022 0.44 -3.8 27,381 + Shewana3_0022 0.47 -0.2
Or see this region's nucleotide sequence