Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4741 and BT4742 are separated by 409 nucleotides BT4742 and BT4743 are separated by 318 nucleotides BT4743 and BT4744 are separated by 389 nucleotides
BT4741: BT4741 - hypothetical protein (NCBI ptt file), at 6,220,589 to 6,221,977
BT4741
BT4742: BT4742 - tetracycline resistance element regulator RteB (NCBI ptt file), at 6,222,387 to 6,222,485
BT4742
BT4743: BT4743 - hypothetical protein (NCBI ptt file), at 6,222,804 to 6,223,052
BT4743
BT4744: BT4744 - putative multiple inositol polyphosphate histidine phosphatase 1 (NCBI ptt file), at 6,223,442 to 6,224,719
BT4744
Position (kb)
6222
6223
6224 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6221.812 kb on + strand, within BT4741 at 6221.813 kb on - strand, within BT4741 at 6221.825 kb on - strand, within BT4741 at 6221.844 kb on - strand at 6221.889 kb on - strand at 6221.889 kb on - strand at 6221.913 kb on + strand at 6221.967 kb on - strand at 6222.013 kb on + strand at 6222.077 kb on + strand at 6222.154 kb on + strand at 6222.155 kb on - strand at 6222.269 kb on + strand at 6222.279 kb on + strand at 6222.279 kb on + strand at 6222.294 kb on + strand at 6222.294 kb on + strand at 6222.354 kb on - strand at 6222.488 kb on + strand at 6222.489 kb on - strand at 6222.494 kb on - strand at 6222.631 kb on - strand at 6222.689 kb on + strand at 6222.705 kb on + strand at 6222.705 kb on + strand at 6222.706 kb on - strand at 6222.706 kb on - strand at 6222.710 kb on + strand at 6222.710 kb on + strand at 6222.865 kb on + strand, within BT4743 at 6222.865 kb on + strand, within BT4743 at 6222.866 kb on - strand, within BT4743 at 6222.866 kb on - strand, within BT4743 at 6222.866 kb on - strand, within BT4743 at 6222.917 kb on - strand, within BT4743 at 6222.942 kb on + strand, within BT4743 at 6222.943 kb on - strand, within BT4743 at 6222.983 kb on + strand, within BT4743 at 6222.990 kb on - strand, within BT4743 at 6223.032 kb on - strand at 6223.032 kb on - strand at 6223.035 kb on - strand at 6223.063 kb on + strand at 6223.079 kb on + strand at 6223.079 kb on + strand at 6223.080 kb on - strand at 6223.081 kb on + strand at 6223.097 kb on + strand at 6223.097 kb on + strand at 6223.098 kb on - strand at 6223.142 kb on + strand at 6223.142 kb on + strand at 6223.155 kb on + strand at 6223.229 kb on - strand at 6223.239 kb on - strand at 6223.263 kb on + strand at 6223.268 kb on - strand at 6223.268 kb on - strand at 6223.268 kb on - strand at 6223.282 kb on - strand at 6223.284 kb on + strand at 6223.284 kb on + strand at 6223.285 kb on - strand at 6223.285 kb on - strand at 6223.285 kb on - strand at 6223.322 kb on + strand at 6223.323 kb on - strand at 6223.324 kb on + strand at 6223.325 kb on - strand at 6223.333 kb on + strand at 6223.333 kb on + strand at 6223.348 kb on - strand at 6223.354 kb on - strand at 6223.367 kb on + strand at 6223.368 kb on - strand at 6223.372 kb on - strand at 6223.439 kb on - strand at 6223.502 kb on - strand at 6223.525 kb on - strand at 6223.544 kb on + strand at 6223.571 kb on + strand, within BT4744 at 6223.582 kb on + strand, within BT4744 at 6223.625 kb on + strand, within BT4744 at 6223.647 kb on - strand, within BT4744 at 6223.712 kb on + strand, within BT4744 at 6223.739 kb on - strand, within BT4744 at 6223.794 kb on - strand, within BT4744 at 6223.888 kb on + strand, within BT4744 at 6223.888 kb on + strand, within BT4744 at 6223.890 kb on + strand, within BT4744 at 6223.890 kb on + strand, within BT4744 at 6224.000 kb on + strand, within BT4744
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 6,221,812 + BT4741 0.88 +0.2 6,221,813 - BT4741 0.88 +0.2 6,221,825 - BT4741 0.89 +0.1 6,221,844 - -1.7 6,221,889 - -0.5 6,221,889 - -3.3 6,221,913 + -0.3 6,221,967 - +1.7 6,222,013 + -3.1 6,222,077 + -2.1 6,222,154 + -0.8 6,222,155 - +0.7 6,222,269 + -1.1 6,222,279 + +0.3 6,222,279 + +0.1 6,222,294 + -1.8 6,222,294 + -2.5 6,222,354 - -0.9 6,222,488 + +0.1 6,222,489 - +2.7 6,222,494 - -0.6 6,222,631 - -0.3 6,222,689 + -0.6 6,222,705 + -0.5 6,222,705 + -1.0 6,222,706 - +0.4 6,222,706 - -2.3 6,222,710 + +0.8 6,222,710 + -0.9 6,222,865 + BT4743 0.24 -2.2 6,222,865 + BT4743 0.24 -0.9 6,222,866 - BT4743 0.25 +0.2 6,222,866 - BT4743 0.25 +0.7 6,222,866 - BT4743 0.25 +0.1 6,222,917 - BT4743 0.45 -2.1 6,222,942 + BT4743 0.55 -2.3 6,222,943 - BT4743 0.56 -2.5 6,222,983 + BT4743 0.72 -1.8 6,222,990 - BT4743 0.75 -2.9 6,223,032 - -0.7 6,223,032 - -3.3 6,223,035 - -1.3 6,223,063 + -0.7 6,223,079 + +0.3 6,223,079 + +1.7 6,223,080 - -0.3 6,223,081 + -0.3 6,223,097 + +0.9 6,223,097 + -1.7 6,223,098 - -0.1 6,223,142 + -1.3 6,223,142 + -1.1 6,223,155 + -0.9 6,223,229 - +0.4 6,223,239 - -1.2 6,223,263 + -0.6 6,223,268 - -1.3 6,223,268 - -2.7 6,223,268 - +0.2 6,223,282 - -0.9 6,223,284 + -2.5 6,223,284 + -0.9 6,223,285 - -0.0 6,223,285 - +0.0 6,223,285 - +0.0 6,223,322 + +1.2 6,223,323 - +0.6 6,223,324 + -2.3 6,223,325 - +0.5 6,223,333 + +0.6 6,223,333 + +0.7 6,223,348 - +0.4 6,223,354 - -1.7 6,223,367 + +0.3 6,223,368 - +1.0 6,223,372 - +0.5 6,223,439 - +0.2 6,223,502 - -2.1 6,223,525 - +1.2 6,223,544 + -0.6 6,223,571 + BT4744 0.10 -2.1 6,223,582 + BT4744 0.11 -0.3 6,223,625 + BT4744 0.14 +0.5 6,223,647 - BT4744 0.16 -1.9 6,223,712 + BT4744 0.21 -1.7 6,223,739 - BT4744 0.23 -1.6 6,223,794 - BT4744 0.28 +0.2 6,223,888 + BT4744 0.35 -3.4 6,223,888 + BT4744 0.35 +0.1 6,223,890 + BT4744 0.35 -2.0 6,223,890 + BT4744 0.35 -0.5 6,224,000 + BT4744 0.44 +0.9
Or see this region's nucleotide sequence