Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4541 and BT4542 overlap by 68 nucleotides BT4542 and BT4543 overlap by 8 nucleotides BT4543 and BT4544 are separated by 43 nucleotides
BT4541: BT4541 - putative Type I restriction modification DNA specificity protein (NCBI ptt file), at 5,962,329 to 5,962,931
BT4541
BT4542: BT4542 - Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file), at 5,962,864 to 5,963,418
BT4542
BT4543: BT4543 - putative type I restriction enzyme specificity protein (NCBI ptt file), at 5,963,411 to 5,964,556
BT4543
BT4544: BT4544 - transposase (NCBI ptt file), at 5,964,600 to 5,965,526
BT4544
Position (kb)
5963
5964
5965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5962.418 kb on + strand, within BT4541 at 5962.419 kb on - strand, within BT4541 at 5962.440 kb on + strand, within BT4541 at 5962.464 kb on - strand, within BT4541 at 5962.468 kb on - strand, within BT4541 at 5962.469 kb on + strand, within BT4541 at 5962.475 kb on + strand, within BT4541 at 5962.476 kb on - strand, within BT4541 at 5962.525 kb on - strand, within BT4541 at 5962.526 kb on + strand, within BT4541 at 5962.556 kb on - strand, within BT4541 at 5962.559 kb on - strand, within BT4541 at 5962.564 kb on + strand, within BT4541 at 5962.587 kb on + strand, within BT4541 at 5962.588 kb on - strand, within BT4541 at 5962.598 kb on + strand, within BT4541 at 5962.598 kb on + strand, within BT4541 at 5962.601 kb on + strand, within BT4541 at 5962.609 kb on - strand, within BT4541 at 5962.623 kb on - strand, within BT4541 at 5962.623 kb on - strand, within BT4541 at 5962.688 kb on + strand, within BT4541 at 5962.892 kb on + strand at 5962.892 kb on + strand at 5962.899 kb on - strand at 5962.946 kb on - strand, within BT4542 at 5962.956 kb on + strand, within BT4542 at 5962.964 kb on - strand, within BT4542 at 5962.966 kb on - strand, within BT4542 at 5962.995 kb on + strand, within BT4542 at 5963.004 kb on - strand, within BT4542 at 5963.067 kb on - strand, within BT4542 at 5963.068 kb on + strand, within BT4542 at 5963.069 kb on - strand, within BT4542 at 5963.103 kb on + strand, within BT4542 at 5963.119 kb on + strand, within BT4542 at 5963.123 kb on + strand, within BT4542 at 5963.167 kb on - strand, within BT4542 at 5963.167 kb on - strand, within BT4542 at 5963.178 kb on + strand, within BT4542 at 5963.207 kb on - strand, within BT4542 at 5963.216 kb on - strand, within BT4542 at 5963.251 kb on + strand, within BT4542 at 5963.264 kb on + strand, within BT4542 at 5963.265 kb on - strand, within BT4542 at 5963.313 kb on - strand, within BT4542 at 5963.333 kb on + strand, within BT4542 at 5963.340 kb on + strand, within BT4542 at 5963.374 kb on + strand at 5963.375 kb on - strand at 5963.392 kb on + strand at 5963.393 kb on - strand at 5963.436 kb on + strand at 5963.437 kb on - strand at 5963.452 kb on + strand at 5963.471 kb on - strand at 5963.473 kb on + strand at 5963.496 kb on - strand at 5963.516 kb on + strand at 5963.549 kb on + strand, within BT4543 at 5963.570 kb on - strand, within BT4543 at 5963.570 kb on - strand, within BT4543 at 5963.610 kb on + strand, within BT4543 at 5963.654 kb on - strand, within BT4543 at 5963.724 kb on - strand, within BT4543 at 5963.800 kb on + strand, within BT4543 at 5963.800 kb on + strand, within BT4543 at 5963.832 kb on - strand, within BT4543 at 5963.843 kb on - strand, within BT4543 at 5963.875 kb on + strand, within BT4543 at 5963.884 kb on + strand, within BT4543 at 5963.956 kb on + strand, within BT4543 at 5964.009 kb on - strand, within BT4543 at 5964.031 kb on + strand, within BT4543 at 5964.163 kb on + strand, within BT4543 at 5964.163 kb on + strand, within BT4543 at 5964.164 kb on - strand, within BT4543 at 5964.164 kb on - strand, within BT4543 at 5964.195 kb on + strand, within BT4543 at 5964.258 kb on + strand, within BT4543 at 5964.377 kb on - strand, within BT4543 at 5964.391 kb on + strand, within BT4543 at 5964.409 kb on + strand, within BT4543 at 5964.410 kb on - strand, within BT4543 at 5964.582 kb on - strand at 5964.588 kb on - strand at 5964.631 kb on + strand at 5964.631 kb on + strand at 5964.657 kb on + strand at 5964.684 kb on + strand at 5964.684 kb on + strand at 5964.692 kb on - strand at 5964.870 kb on + strand, within BT4544 at 5964.892 kb on - strand, within BT4544 at 5964.929 kb on - strand, within BT4544 at 5965.099 kb on + strand, within BT4544 at 5965.108 kb on + strand, within BT4544 at 5965.108 kb on + strand, within BT4544 at 5965.111 kb on - strand, within BT4544 at 5965.302 kb on + strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.303 kb on - strand, within BT4544 at 5965.315 kb on + strand, within BT4544 at 5965.316 kb on - strand, within BT4544 at 5965.384 kb on + strand, within BT4544 at 5965.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 5,962,418 + BT4541 0.15 +0.2 5,962,419 - BT4541 0.15 -0.2 5,962,440 + BT4541 0.18 -2.2 5,962,464 - BT4541 0.22 -0.4 5,962,468 - BT4541 0.23 -0.4 5,962,469 + BT4541 0.23 -1.9 5,962,475 + BT4541 0.24 +0.1 5,962,476 - BT4541 0.24 -2.4 5,962,525 - BT4541 0.33 -0.8 5,962,526 + BT4541 0.33 -1.0 5,962,556 - BT4541 0.38 +1.0 5,962,559 - BT4541 0.38 -1.8 5,962,564 + BT4541 0.39 -0.4 5,962,587 + BT4541 0.43 +1.0 5,962,588 - BT4541 0.43 -2.1 5,962,598 + BT4541 0.45 +0.1 5,962,598 + BT4541 0.45 +1.4 5,962,601 + BT4541 0.45 +0.6 5,962,609 - BT4541 0.46 -2.2 5,962,623 - BT4541 0.49 -2.7 5,962,623 - BT4541 0.49 +1.3 5,962,688 + BT4541 0.60 +0.2 5,962,892 + -1.2 5,962,892 + -0.3 5,962,899 - +0.2 5,962,946 - BT4542 0.15 -0.0 5,962,956 + BT4542 0.17 -0.6 5,962,964 - BT4542 0.18 +0.6 5,962,966 - BT4542 0.18 -0.2 5,962,995 + BT4542 0.24 -0.4 5,963,004 - BT4542 0.25 -1.0 5,963,067 - BT4542 0.37 +2.6 5,963,068 + BT4542 0.37 -1.2 5,963,069 - BT4542 0.37 +0.8 5,963,103 + BT4542 0.43 +0.6 5,963,119 + BT4542 0.46 +1.4 5,963,123 + BT4542 0.47 -2.9 5,963,167 - BT4542 0.55 -0.5 5,963,167 - BT4542 0.55 -2.2 5,963,178 + BT4542 0.57 -0.6 5,963,207 - BT4542 0.62 +0.1 5,963,216 - BT4542 0.63 -1.9 5,963,251 + BT4542 0.70 -1.9 5,963,264 + BT4542 0.72 -0.9 5,963,265 - BT4542 0.72 +0.5 5,963,313 - BT4542 0.81 -0.2 5,963,333 + BT4542 0.85 +0.7 5,963,340 + BT4542 0.86 +2.0 5,963,374 + -0.2 5,963,375 - -3.1 5,963,392 + +0.8 5,963,393 - -0.5 5,963,436 + +0.0 5,963,437 - -0.6 5,963,452 + +0.8 5,963,471 - -1.3 5,963,473 + -3.1 5,963,496 - +1.4 5,963,516 + -0.5 5,963,549 + BT4543 0.12 -3.6 5,963,570 - BT4543 0.14 -0.2 5,963,570 - BT4543 0.14 -0.4 5,963,610 + BT4543 0.17 -1.7 5,963,654 - BT4543 0.21 -0.1 5,963,724 - BT4543 0.27 -1.0 5,963,800 + BT4543 0.34 -2.3 5,963,800 + BT4543 0.34 +0.2 5,963,832 - BT4543 0.37 -1.1 5,963,843 - BT4543 0.38 -1.3 5,963,875 + BT4543 0.40 -0.9 5,963,884 + BT4543 0.41 -0.1 5,963,956 + BT4543 0.48 -0.9 5,964,009 - BT4543 0.52 -2.0 5,964,031 + BT4543 0.54 -1.1 5,964,163 + BT4543 0.66 -1.2 5,964,163 + BT4543 0.66 -0.9 5,964,164 - BT4543 0.66 +1.1 5,964,164 - BT4543 0.66 -1.9 5,964,195 + BT4543 0.68 -1.9 5,964,258 + BT4543 0.74 +0.5 5,964,377 - BT4543 0.84 -1.4 5,964,391 + BT4543 0.86 -2.4 5,964,409 + BT4543 0.87 +0.1 5,964,410 - BT4543 0.87 -2.8 5,964,582 - +0.1 5,964,588 - +0.9 5,964,631 + -0.1 5,964,631 + +0.8 5,964,657 + +0.6 5,964,684 + +0.3 5,964,684 + -0.6 5,964,692 - +0.1 5,964,870 + BT4544 0.29 -2.2 5,964,892 - BT4544 0.31 -1.0 5,964,929 - BT4544 0.35 +0.1 5,965,099 + BT4544 0.54 +0.1 5,965,108 + BT4544 0.55 +0.7 5,965,108 + BT4544 0.55 +0.4 5,965,111 - BT4544 0.55 +1.2 5,965,302 + BT4544 0.76 -2.7 5,965,303 - BT4544 0.76 -2.1 5,965,303 - BT4544 0.76 -0.5 5,965,315 + BT4544 0.77 -1.4 5,965,316 - BT4544 0.77 -0.7 5,965,384 + BT4544 0.85 -0.5 5,965,458 + -1.6
Or see this region's nucleotide sequence