Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2647 and BT2648 are separated by 12 nucleotides BT2648 and BT2649 overlap by 4 nucleotides BT2649 and BT2650 are separated by 97 nucleotides
BT2647: BT2647 - hypothetical protein (NCBI ptt file), at 3,299,935 to 3,301,041
BT2647
BT2648: BT2648 - hypothetical protein (NCBI ptt file), at 3,301,054 to 3,301,755
BT2648
BT2649: BT2649 - ThiF family protein, ubiquitin-activating enzyme (NCBI ptt file), at 3,301,752 to 3,302,549
BT2649
BT2650: BT2650 - conserved hypothetical protein (NCBI ptt file), at 3,302,647 to 3,303,348
BT2650
Position (kb)
3301
3302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3300.096 kb on + strand, within BT2647 at 3300.190 kb on + strand, within BT2647 at 3300.284 kb on + strand, within BT2647 at 3300.328 kb on + strand, within BT2647 at 3300.329 kb on - strand, within BT2647 at 3300.367 kb on - strand, within BT2647 at 3300.426 kb on - strand, within BT2647 at 3300.511 kb on + strand, within BT2647 at 3300.533 kb on - strand, within BT2647 at 3300.669 kb on - strand, within BT2647 at 3300.669 kb on - strand, within BT2647 at 3300.682 kb on - strand, within BT2647 at 3300.737 kb on - strand, within BT2647 at 3300.737 kb on - strand, within BT2647 at 3300.737 kb on - strand, within BT2647 at 3300.746 kb on - strand, within BT2647 at 3300.769 kb on + strand, within BT2647 at 3300.770 kb on - strand, within BT2647 at 3300.770 kb on - strand, within BT2647 at 3300.787 kb on + strand, within BT2647 at 3300.788 kb on - strand, within BT2647 at 3300.910 kb on + strand, within BT2647 at 3301.039 kb on + strand at 3301.103 kb on + strand at 3301.213 kb on + strand, within BT2648 at 3301.213 kb on + strand, within BT2648 at 3301.327 kb on + strand, within BT2648 at 3301.331 kb on + strand, within BT2648 at 3301.331 kb on + strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand at 3301.332 kb on - strand, within BT2648 at 3301.332 kb on - strand, within BT2648 at 3301.337 kb on - strand, within BT2648 at 3301.420 kb on + strand, within BT2648 at 3301.420 kb on + strand, within BT2648 at 3301.448 kb on + strand, within BT2648 at 3301.448 kb on + strand, within BT2648 at 3301.449 kb on - strand, within BT2648 at 3301.449 kb on - strand, within BT2648 at 3301.492 kb on + strand, within BT2648 at 3301.493 kb on - strand, within BT2648 at 3301.715 kb on + strand at 3301.745 kb on + strand at 3301.767 kb on + strand at 3301.780 kb on - strand at 3301.795 kb on - strand at 3301.903 kb on + strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.904 kb on - strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.948 kb on + strand, within BT2649 at 3301.949 kb on - strand, within BT2649 at 3302.066 kb on - strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.260 kb on + strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.261 kb on - strand, within BT2649 at 3302.282 kb on - strand, within BT2649 at 3302.350 kb on - strand, within BT2649 at 3302.422 kb on - strand, within BT2649 at 3302.434 kb on + strand, within BT2649 at 3302.461 kb on + strand, within BT2649 at 3302.462 kb on - strand, within BT2649 at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.505 kb on + strand at 3302.506 kb on - strand at 3302.506 kb on - strand at 3302.507 kb on + strand at 3302.545 kb on + strand at 3302.548 kb on - strand at 3302.628 kb on + strand at 3302.629 kb on - strand at 3302.721 kb on - strand, within BT2650 at 3302.748 kb on - strand, within BT2650
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 3,300,096 + BT2647 0.15 -0.2 3,300,190 + BT2647 0.23 -1.6 3,300,284 + BT2647 0.32 -1.0 3,300,328 + BT2647 0.36 -3.6 3,300,329 - BT2647 0.36 +0.2 3,300,367 - BT2647 0.39 -0.5 3,300,426 - BT2647 0.44 +1.4 3,300,511 + BT2647 0.52 +0.6 3,300,533 - BT2647 0.54 -2.4 3,300,669 - BT2647 0.66 +1.0 3,300,669 - BT2647 0.66 -2.4 3,300,682 - BT2647 0.67 -1.1 3,300,737 - BT2647 0.72 +0.7 3,300,737 - BT2647 0.72 -0.8 3,300,737 - BT2647 0.72 -2.4 3,300,746 - BT2647 0.73 -0.6 3,300,769 + BT2647 0.75 +0.3 3,300,770 - BT2647 0.75 -2.8 3,300,770 - BT2647 0.75 +1.0 3,300,787 + BT2647 0.77 -0.5 3,300,788 - BT2647 0.77 -0.8 3,300,910 + BT2647 0.88 +1.8 3,301,039 + -0.3 3,301,103 + +0.8 3,301,213 + BT2648 0.23 -1.2 3,301,213 + BT2648 0.23 -1.3 3,301,327 + BT2648 0.39 +1.0 3,301,331 + BT2648 0.39 -2.4 3,301,331 + BT2648 0.39 -0.9 3,301,332 - BT2648 0.40 -3.9 3,301,332 - BT2648 0.40 -1.3 3,301,332 - BT2648 0.40 -0.7 3,301,332 - -1.0 3,301,332 - BT2648 0.40 -2.3 3,301,332 - BT2648 0.40 -0.3 3,301,337 - BT2648 0.40 +0.6 3,301,420 + BT2648 0.52 -0.5 3,301,420 + BT2648 0.52 -0.1 3,301,448 + BT2648 0.56 +1.4 3,301,448 + BT2648 0.56 -0.3 3,301,449 - BT2648 0.56 -1.5 3,301,449 - BT2648 0.56 -0.8 3,301,492 + BT2648 0.62 -2.4 3,301,493 - BT2648 0.63 -1.3 3,301,715 + +0.6 3,301,745 + +0.9 3,301,767 + -1.8 3,301,780 - -2.2 3,301,795 - -0.8 3,301,903 + BT2649 0.19 -0.3 3,301,904 - BT2649 0.19 +0.5 3,301,904 - BT2649 0.19 +0.7 3,301,948 + BT2649 0.25 +0.0 3,301,948 + BT2649 0.25 +1.0 3,301,949 - BT2649 0.25 +0.0 3,302,066 - BT2649 0.39 +0.4 3,302,260 + BT2649 0.64 -0.8 3,302,260 + BT2649 0.64 -3.5 3,302,261 - BT2649 0.64 -0.5 3,302,261 - BT2649 0.64 -0.5 3,302,282 - BT2649 0.66 -0.9 3,302,350 - BT2649 0.75 +0.0 3,302,422 - BT2649 0.84 +1.2 3,302,434 + BT2649 0.85 -1.0 3,302,461 + BT2649 0.89 +0.9 3,302,462 - BT2649 0.89 -2.7 3,302,505 + -2.3 3,302,505 + +1.6 3,302,505 + -2.4 3,302,505 + +0.8 3,302,505 + -0.6 3,302,506 - +2.5 3,302,506 - +0.6 3,302,507 + -0.5 3,302,545 + +1.0 3,302,548 - -2.1 3,302,628 + +0.8 3,302,629 - +0.4 3,302,721 - BT2650 0.11 -1.0 3,302,748 - BT2650 0.14 +0.4
Or see this region's nucleotide sequence