Experiment: BHIS with Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2017 and BT2018 are separated by 77 nucleotides BT2018 and BT2019 are separated by 55 nucleotides BT2019 and BT2020 are separated by 72 nucleotides
BT2017: BT2017 - glucose-1-phosphate thymidylyltransferase (NCBI ptt file), at 2,531,321 to 2,532,196
BT2017
BT2018: BT2018 - conserved hypothetical protein (NCBI ptt file), at 2,532,274 to 2,532,765
BT2018
BT2019: BT2019 - polyphosphate kinase (NCBI ptt file), at 2,532,821 to 2,534,899
BT2019
BT2020: BT2020 - putative phosphate/sulphate permeases (NCBI ptt file), at 2,534,972 to 2,537,224
BT2020
Position (kb)
2532
2533
2534
2535 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2532.427 kb on + strand, within BT2018 at 2532.427 kb on + strand, within BT2018 at 2532.428 kb on - strand, within BT2018 at 2532.428 kb on - strand, within BT2018 at 2532.428 kb on - strand, within BT2018 at 2532.497 kb on + strand, within BT2018 at 2532.520 kb on - strand, within BT2018 at 2532.583 kb on + strand, within BT2018 at 2532.599 kb on - strand, within BT2018 at 2532.666 kb on + strand, within BT2018 at 2532.713 kb on + strand, within BT2018 at 2532.729 kb on - strand at 2532.796 kb on + strand at 2532.796 kb on + strand at 2532.798 kb on + strand at 2532.814 kb on - strand at 2532.944 kb on + strand at 2533.157 kb on + strand, within BT2019 at 2533.352 kb on + strand, within BT2019 at 2533.424 kb on + strand, within BT2019 at 2533.761 kb on + strand, within BT2019 at 2533.830 kb on - strand, within BT2019 at 2534.019 kb on + strand, within BT2019 at 2534.020 kb on - strand, within BT2019 at 2534.020 kb on - strand, within BT2019 at 2534.121 kb on + strand, within BT2019 at 2534.186 kb on + strand, within BT2019 at 2534.234 kb on + strand, within BT2019 at 2534.286 kb on - strand, within BT2019 at 2534.425 kb on + strand, within BT2019 at 2534.426 kb on - strand, within BT2019 at 2534.426 kb on - strand, within BT2019 at 2534.553 kb on - strand, within BT2019 at 2534.616 kb on - strand, within BT2019 at 2534.616 kb on - strand, within BT2019 at 2534.905 kb on - strand at 2534.958 kb on - strand at 2534.985 kb on - strand at 2534.989 kb on - strand at 2534.989 kb on - strand at 2535.171 kb on + strand at 2535.171 kb on + strand at 2535.172 kb on - strand at 2535.207 kb on - strand, within BT2020 at 2535.227 kb on - strand, within BT2020 at 2535.227 kb on - strand, within BT2020 at 2535.228 kb on + strand, within BT2020 at 2535.229 kb on - strand, within BT2020 at 2535.466 kb on + strand, within BT2020 at 2535.471 kb on + strand, within BT2020 at 2535.471 kb on + strand, within BT2020 at 2535.472 kb on - strand, within BT2020 at 2535.509 kb on + strand, within BT2020 at 2535.832 kb on - strand, within BT2020 at 2535.836 kb on + strand, within BT2020 at 2535.836 kb on + strand, within BT2020
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml remove 2,532,427 + BT2018 0.31 +3.0 2,532,427 + BT2018 0.31 +0.3 2,532,428 - BT2018 0.31 +1.1 2,532,428 - BT2018 0.31 +1.2 2,532,428 - BT2018 0.31 +0.5 2,532,497 + BT2018 0.45 -2.4 2,532,520 - BT2018 0.50 -1.2 2,532,583 + BT2018 0.63 +1.1 2,532,599 - BT2018 0.66 +0.5 2,532,666 + BT2018 0.80 -0.9 2,532,713 + BT2018 0.89 -2.5 2,532,729 - -0.0 2,532,796 + -1.2 2,532,796 + +0.6 2,532,798 + -2.8 2,532,814 - -2.4 2,532,944 + -0.4 2,533,157 + BT2019 0.16 +0.4 2,533,352 + BT2019 0.26 -1.7 2,533,424 + BT2019 0.29 -2.2 2,533,761 + BT2019 0.45 -2.2 2,533,830 - BT2019 0.49 -3.5 2,534,019 + BT2019 0.58 -2.0 2,534,020 - BT2019 0.58 -0.4 2,534,020 - BT2019 0.58 +0.8 2,534,121 + BT2019 0.63 -1.2 2,534,186 + BT2019 0.66 +0.4 2,534,234 + BT2019 0.68 -0.2 2,534,286 - BT2019 0.70 -1.3 2,534,425 + BT2019 0.77 -1.7 2,534,426 - BT2019 0.77 -2.7 2,534,426 - BT2019 0.77 -2.8 2,534,553 - BT2019 0.83 -2.4 2,534,616 - BT2019 0.86 -0.2 2,534,616 - BT2019 0.86 -2.3 2,534,905 - +0.5 2,534,958 - -1.7 2,534,985 - +0.3 2,534,989 - -0.0 2,534,989 - +1.0 2,535,171 + -0.5 2,535,171 + -2.4 2,535,172 - +0.8 2,535,207 - BT2020 0.10 +1.2 2,535,227 - BT2020 0.11 -1.8 2,535,227 - BT2020 0.11 +0.8 2,535,228 + BT2020 0.11 -0.4 2,535,229 - BT2020 0.11 +0.1 2,535,466 + BT2020 0.22 -2.9 2,535,471 + BT2020 0.22 +0.9 2,535,471 + BT2020 0.22 +0.4 2,535,472 - BT2020 0.22 -1.7 2,535,509 + BT2020 0.24 +0.3 2,535,832 - BT2020 0.38 -1.5 2,535,836 + BT2020 0.38 +0.4 2,535,836 + BT2020 0.38 -2.0
Or see this region's nucleotide sequence