Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2019

Experiment: BHIS with Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2017 and BT2018 are separated by 77 nucleotidesBT2018 and BT2019 are separated by 55 nucleotidesBT2019 and BT2020 are separated by 72 nucleotides BT2017: BT2017 - glucose-1-phosphate thymidylyltransferase (NCBI ptt file), at 2,531,321 to 2,532,196 BT2017 BT2018: BT2018 - conserved hypothetical protein (NCBI ptt file), at 2,532,274 to 2,532,765 BT2018 BT2019: BT2019 - polyphosphate kinase (NCBI ptt file), at 2,532,821 to 2,534,899 BT2019 BT2020: BT2020 - putative phosphate/sulphate permeases (NCBI ptt file), at 2,534,972 to 2,537,224 BT2020 Position (kb) 2532 2533 2534 2535Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2532.427 kb on + strand, within BT2018at 2532.427 kb on + strand, within BT2018at 2532.428 kb on - strand, within BT2018at 2532.428 kb on - strand, within BT2018at 2532.428 kb on - strand, within BT2018at 2532.497 kb on + strand, within BT2018at 2532.520 kb on - strand, within BT2018at 2532.583 kb on + strand, within BT2018at 2532.599 kb on - strand, within BT2018at 2532.666 kb on + strand, within BT2018at 2532.713 kb on + strand, within BT2018at 2532.729 kb on - strandat 2532.796 kb on + strandat 2532.796 kb on + strandat 2532.798 kb on + strandat 2532.814 kb on - strandat 2532.944 kb on + strandat 2533.157 kb on + strand, within BT2019at 2533.352 kb on + strand, within BT2019at 2533.424 kb on + strand, within BT2019at 2533.761 kb on + strand, within BT2019at 2533.830 kb on - strand, within BT2019at 2534.019 kb on + strand, within BT2019at 2534.020 kb on - strand, within BT2019at 2534.020 kb on - strand, within BT2019at 2534.121 kb on + strand, within BT2019at 2534.186 kb on + strand, within BT2019at 2534.234 kb on + strand, within BT2019at 2534.286 kb on - strand, within BT2019at 2534.425 kb on + strand, within BT2019at 2534.426 kb on - strand, within BT2019at 2534.426 kb on - strand, within BT2019at 2534.553 kb on - strand, within BT2019at 2534.616 kb on - strand, within BT2019at 2534.616 kb on - strand, within BT2019at 2534.905 kb on - strandat 2534.958 kb on - strandat 2534.985 kb on - strandat 2534.989 kb on - strandat 2534.989 kb on - strandat 2535.171 kb on + strandat 2535.171 kb on + strandat 2535.172 kb on - strandat 2535.207 kb on - strand, within BT2020at 2535.227 kb on - strand, within BT2020at 2535.227 kb on - strand, within BT2020at 2535.228 kb on + strand, within BT2020at 2535.229 kb on - strand, within BT2020at 2535.466 kb on + strand, within BT2020at 2535.471 kb on + strand, within BT2020at 2535.471 kb on + strand, within BT2020at 2535.472 kb on - strand, within BT2020at 2535.509 kb on + strand, within BT2020at 2535.832 kb on - strand, within BT2020at 2535.836 kb on + strand, within BT2020at 2535.836 kb on + strand, within BT2020

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Paraquat dichloride 0.1 mg/ml
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2,532,427 + BT2018 0.31 +3.0
2,532,427 + BT2018 0.31 +0.3
2,532,428 - BT2018 0.31 +1.1
2,532,428 - BT2018 0.31 +1.2
2,532,428 - BT2018 0.31 +0.5
2,532,497 + BT2018 0.45 -2.4
2,532,520 - BT2018 0.50 -1.2
2,532,583 + BT2018 0.63 +1.1
2,532,599 - BT2018 0.66 +0.5
2,532,666 + BT2018 0.80 -0.9
2,532,713 + BT2018 0.89 -2.5
2,532,729 - -0.0
2,532,796 + -1.2
2,532,796 + +0.6
2,532,798 + -2.8
2,532,814 - -2.4
2,532,944 + -0.4
2,533,157 + BT2019 0.16 +0.4
2,533,352 + BT2019 0.26 -1.7
2,533,424 + BT2019 0.29 -2.2
2,533,761 + BT2019 0.45 -2.2
2,533,830 - BT2019 0.49 -3.5
2,534,019 + BT2019 0.58 -2.0
2,534,020 - BT2019 0.58 -0.4
2,534,020 - BT2019 0.58 +0.8
2,534,121 + BT2019 0.63 -1.2
2,534,186 + BT2019 0.66 +0.4
2,534,234 + BT2019 0.68 -0.2
2,534,286 - BT2019 0.70 -1.3
2,534,425 + BT2019 0.77 -1.7
2,534,426 - BT2019 0.77 -2.7
2,534,426 - BT2019 0.77 -2.8
2,534,553 - BT2019 0.83 -2.4
2,534,616 - BT2019 0.86 -0.2
2,534,616 - BT2019 0.86 -2.3
2,534,905 - +0.5
2,534,958 - -1.7
2,534,985 - +0.3
2,534,989 - -0.0
2,534,989 - +1.0
2,535,171 + -0.5
2,535,171 + -2.4
2,535,172 - +0.8
2,535,207 - BT2020 0.10 +1.2
2,535,227 - BT2020 0.11 -1.8
2,535,227 - BT2020 0.11 +0.8
2,535,228 + BT2020 0.11 -0.4
2,535,229 - BT2020 0.11 +0.1
2,535,466 + BT2020 0.22 -2.9
2,535,471 + BT2020 0.22 +0.9
2,535,471 + BT2020 0.22 +0.4
2,535,472 - BT2020 0.22 -1.7
2,535,509 + BT2020 0.24 +0.3
2,535,832 - BT2020 0.38 -1.5
2,535,836 + BT2020 0.38 +0.4
2,535,836 + BT2020 0.38 -2.0

Or see this region's nucleotide sequence