Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08935 and MMJJ_RS08940 are separated by 35 nucleotides MMJJ_RS08940 and MMJJ_RS09375 are separated by 13 nucleotides MMJJ_RS09375 and MMJJ_RS08945 are separated by 7 nucleotides MMJJ_RS08945 and MMJJ_RS08950 are separated by 55 nucleotides MMJJ_RS08950 and MMJJ_RS08955 are separated by 21 nucleotides
MMJJ_RS08935: MMJJ_RS08935 - TIGR00288 family NYN domain-containing protein, at 1,648,067 to 1,648,714
_RS08935
MMJJ_RS08940: MMJJ_RS08940 - monovalent cation/H+ antiporter complex subunit F, at 1,648,750 to 1,649,019
_RS08940
MMJJ_RS09375: MMJJ_RS09375 - hypothetical protein, at 1,649,033 to 1,649,209
_RS09375
MMJJ_RS08945: MMJJ_RS08945 - DUF61 family protein, at 1,649,217 to 1,649,606
_RS08945
MMJJ_RS08950: MMJJ_RS08950 - DUF22 domain-containing protein, at 1,649,662 to 1,650,027
_RS08950
MMJJ_RS08955: MMJJ_RS08955 - V-type ATP synthase subunit D, at 1,650,049 to 1,650,693
_RS08955
Position (kb)
1649
1650 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1648.064 kb on - strand at 1648.367 kb on + strand, within MMJJ_RS08935 at 1648.515 kb on - strand at 1648.589 kb on + strand, within MMJJ_RS08935 at 1648.672 kb on - strand at 1648.672 kb on - strand at 1648.696 kb on + strand at 1648.733 kb on - strand at 1648.734 kb on + strand at 1648.734 kb on + strand at 1648.738 kb on + strand at 1648.742 kb on - strand at 1648.751 kb on - strand at 1648.752 kb on + strand at 1648.754 kb on + strand at 1648.877 kb on + strand, within MMJJ_RS08940 at 1648.924 kb on - strand, within MMJJ_RS08940 at 1648.925 kb on + strand, within MMJJ_RS08940 at 1649.000 kb on - strand at 1649.006 kb on + strand at 1649.030 kb on + strand at 1649.071 kb on - strand, within MMJJ_RS09375 at 1649.105 kb on - strand, within MMJJ_RS09375 at 1649.107 kb on - strand at 1649.112 kb on + strand at 1649.123 kb on - strand, within MMJJ_RS09375 at 1649.218 kb on - strand at 1649.222 kb on - strand at 1649.229 kb on - strand at 1649.238 kb on - strand at 1649.252 kb on - strand at 1649.253 kb on + strand at 1649.304 kb on - strand, within MMJJ_RS08945 at 1649.306 kb on - strand, within MMJJ_RS08945 at 1649.307 kb on + strand, within MMJJ_RS08945 at 1649.380 kb on + strand at 1649.418 kb on - strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.419 kb on + strand, within MMJJ_RS08945 at 1649.421 kb on - strand, within MMJJ_RS08945 at 1649.424 kb on - strand, within MMJJ_RS08945 at 1649.424 kb on - strand, within MMJJ_RS08945 at 1649.425 kb on + strand, within MMJJ_RS08945 at 1649.425 kb on + strand, within MMJJ_RS08945 at 1649.436 kb on - strand, within MMJJ_RS08945 at 1649.436 kb on - strand, within MMJJ_RS08945 at 1649.437 kb on + strand, within MMJJ_RS08945 at 1649.437 kb on + strand, within MMJJ_RS08945 at 1649.509 kb on + strand, within MMJJ_RS08945 at 1649.523 kb on + strand, within MMJJ_RS08945 at 1649.624 kb on + strand at 1649.634 kb on + strand at 1649.644 kb on + strand at 1649.655 kb on - strand at 1649.655 kb on - strand at 1649.659 kb on - strand at 1649.659 kb on - strand at 1649.660 kb on + strand at 1649.678 kb on + strand at 1649.680 kb on - strand at 1649.680 kb on - strand at 1649.680 kb on - strand at 1649.681 kb on + strand at 1649.683 kb on - strand at 1649.683 kb on - strand at 1649.684 kb on + strand at 1649.684 kb on + strand at 1649.710 kb on + strand, within MMJJ_RS08950 at 1649.712 kb on - strand, within MMJJ_RS08950 at 1649.713 kb on + strand, within MMJJ_RS08950 at 1649.725 kb on - strand, within MMJJ_RS08950 at 1649.737 kb on - strand, within MMJJ_RS08950 at 1649.738 kb on + strand, within MMJJ_RS08950 at 1649.744 kb on - strand, within MMJJ_RS08950 at 1649.825 kb on - strand, within MMJJ_RS08950 at 1649.826 kb on + strand, within MMJJ_RS08950 at 1649.947 kb on - strand, within MMJJ_RS08950 at 1649.948 kb on + strand, within MMJJ_RS08950 at 1649.953 kb on - strand, within MMJJ_RS08950
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,648,064 - -2.4 1,648,367 + MMJJ_RS08935 0.46 +1.4 1,648,515 - -1.6 1,648,589 + MMJJ_RS08935 0.81 -1.0 1,648,672 - -1.5 1,648,672 - +0.3 1,648,696 + -1.6 1,648,733 - +0.5 1,648,734 + +0.2 1,648,734 + +0.4 1,648,738 + +1.3 1,648,742 - -0.4 1,648,751 - +0.7 1,648,752 + -0.7 1,648,754 + -0.2 1,648,877 + MMJJ_RS08940 0.47 -1.8 1,648,924 - MMJJ_RS08940 0.64 -2.5 1,648,925 + MMJJ_RS08940 0.65 -2.4 1,649,000 - -2.2 1,649,006 + -4.7 1,649,030 + -2.4 1,649,071 - MMJJ_RS09375 0.21 -2.0 1,649,105 - MMJJ_RS09375 0.41 -3.2 1,649,107 - -0.8 1,649,112 + -0.8 1,649,123 - MMJJ_RS09375 0.51 -1.7 1,649,218 - +0.1 1,649,222 - -0.8 1,649,229 - -1.6 1,649,238 - -0.0 1,649,252 - +0.8 1,649,253 + -0.4 1,649,304 - MMJJ_RS08945 0.22 +0.2 1,649,306 - MMJJ_RS08945 0.23 -1.3 1,649,307 + MMJJ_RS08945 0.23 +0.3 1,649,380 + +2.2 1,649,418 - MMJJ_RS08945 0.52 +0.8 1,649,419 + MMJJ_RS08945 0.52 -1.4 1,649,419 + MMJJ_RS08945 0.52 -0.1 1,649,419 + MMJJ_RS08945 0.52 -1.2 1,649,421 - MMJJ_RS08945 0.52 +0.4 1,649,424 - MMJJ_RS08945 0.53 -1.3 1,649,424 - MMJJ_RS08945 0.53 -0.2 1,649,425 + MMJJ_RS08945 0.53 -0.9 1,649,425 + MMJJ_RS08945 0.53 +2.6 1,649,436 - MMJJ_RS08945 0.56 -2.2 1,649,436 - MMJJ_RS08945 0.56 -0.4 1,649,437 + MMJJ_RS08945 0.56 -1.1 1,649,437 + MMJJ_RS08945 0.56 -1.3 1,649,509 + MMJJ_RS08945 0.75 -0.2 1,649,523 + MMJJ_RS08945 0.78 -0.4 1,649,624 + +0.5 1,649,634 + -0.7 1,649,644 + +0.1 1,649,655 - -0.7 1,649,655 - -0.9 1,649,659 - +0.2 1,649,659 - +0.4 1,649,660 + -1.8 1,649,678 + -1.6 1,649,680 - +0.3 1,649,680 - +2.2 1,649,680 - +0.7 1,649,681 + -1.3 1,649,683 - -1.3 1,649,683 - -0.1 1,649,684 + -1.0 1,649,684 + +0.7 1,649,710 + MMJJ_RS08950 0.13 -0.2 1,649,712 - MMJJ_RS08950 0.14 +1.0 1,649,713 + MMJJ_RS08950 0.14 -0.8 1,649,725 - MMJJ_RS08950 0.17 +0.3 1,649,737 - MMJJ_RS08950 0.20 +0.9 1,649,738 + MMJJ_RS08950 0.21 +1.4 1,649,744 - MMJJ_RS08950 0.22 -0.5 1,649,825 - MMJJ_RS08950 0.45 +0.4 1,649,826 + MMJJ_RS08950 0.45 -0.5 1,649,947 - MMJJ_RS08950 0.78 +0.8 1,649,948 + MMJJ_RS08950 0.78 +1.9 1,649,953 - MMJJ_RS08950 0.80 -0.2
Or see this region's nucleotide sequence