Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09180 and MMJJ_RS09185 are separated by 10 nucleotides MMJJ_RS09185 and MMJJ_RS09190 overlap by 11 nucleotides MMJJ_RS09190 and MMJJ_RS09195 are separated by 0 nucleotides MMJJ_RS09195 and MMJJ_RS09200 are separated by 26 nucleotides
MMJJ_RS09180: MMJJ_RS09180 - aminodeoxychorismate/anthranilate synthase component II, at 1,704,120 to 1,704,716
_RS09180
MMJJ_RS09185: MMJJ_RS09185 - phosphoribosylanthranilate isomerase, at 1,704,727 to 1,705,362
_RS09185
MMJJ_RS09190: MMJJ_RS09190 - tryptophan synthase subunit beta, at 1,705,352 to 1,706,536
_RS09190
MMJJ_RS09195: MMJJ_RS09195 - tryptophan synthase subunit alpha, at 1,706,537 to 1,707,313
_RS09195
MMJJ_RS09200: MMJJ_RS09200 - Nre family DNA repair protein, at 1,707,340 to 1,708,560
_RS09200
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1704.492 kb on - strand, within MMJJ_RS09180 at 1705.351 kb on - strand at 1705.790 kb on + strand, within MMJJ_RS09190 at 1705.995 kb on - strand at 1706.038 kb on - strand at 1706.081 kb on + strand, within MMJJ_RS09190 at 1706.292 kb on + strand, within MMJJ_RS09190 at 1707.183 kb on - strand, within MMJJ_RS09195 at 1707.184 kb on + strand, within MMJJ_RS09195 at 1707.209 kb on + strand, within MMJJ_RS09195 at 1707.212 kb on + strand, within MMJJ_RS09195 at 1707.311 kb on - strand at 1707.312 kb on + strand at 1707.342 kb on + strand at 1707.352 kb on + strand at 1707.390 kb on - strand at 1707.391 kb on + strand at 1707.404 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,704,492 - MMJJ_RS09180 0.62 -0.8 1,705,351 - -2.6 1,705,790 + MMJJ_RS09190 0.37 -1.2 1,705,995 - -0.8 1,706,038 - -0.8 1,706,081 + MMJJ_RS09190 0.62 -3.3 1,706,292 + MMJJ_RS09190 0.79 -2.0 1,707,183 - MMJJ_RS09195 0.83 +0.4 1,707,184 + MMJJ_RS09195 0.83 -1.2 1,707,209 + MMJJ_RS09195 0.86 -1.2 1,707,212 + MMJJ_RS09195 0.87 -2.7 1,707,311 - +1.4 1,707,312 + -0.4 1,707,342 + +0.3 1,707,352 + +0.7 1,707,390 - +0.3 1,707,391 + +1.6 1,707,404 - +0.6
Or see this region's nucleotide sequence