Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS09190

Experiment: H2 N-free with 10mM Ammonium chloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS09180 and MMJJ_RS09185 are separated by 10 nucleotidesMMJJ_RS09185 and MMJJ_RS09190 overlap by 11 nucleotidesMMJJ_RS09190 and MMJJ_RS09195 are separated by 0 nucleotidesMMJJ_RS09195 and MMJJ_RS09200 are separated by 26 nucleotides MMJJ_RS09180: MMJJ_RS09180 - aminodeoxychorismate/anthranilate synthase component II, at 1,704,120 to 1,704,716 _RS09180 MMJJ_RS09185: MMJJ_RS09185 - phosphoribosylanthranilate isomerase, at 1,704,727 to 1,705,362 _RS09185 MMJJ_RS09190: MMJJ_RS09190 - tryptophan synthase subunit beta, at 1,705,352 to 1,706,536 _RS09190 MMJJ_RS09195: MMJJ_RS09195 - tryptophan synthase subunit alpha, at 1,706,537 to 1,707,313 _RS09195 MMJJ_RS09200: MMJJ_RS09200 - Nre family DNA repair protein, at 1,707,340 to 1,708,560 _RS09200 Position (kb) 1705 1706 1707Strain fitness (log2 ratio) -3 -2 -1 0 1at 1704.492 kb on - strand, within MMJJ_RS09180at 1705.351 kb on - strandat 1705.790 kb on + strand, within MMJJ_RS09190at 1705.995 kb on - strandat 1706.038 kb on - strandat 1706.081 kb on + strand, within MMJJ_RS09190at 1706.292 kb on + strand, within MMJJ_RS09190at 1707.183 kb on - strand, within MMJJ_RS09195at 1707.184 kb on + strand, within MMJJ_RS09195at 1707.209 kb on + strand, within MMJJ_RS09195at 1707.212 kb on + strand, within MMJJ_RS09195at 1707.311 kb on - strandat 1707.312 kb on + strandat 1707.342 kb on + strandat 1707.352 kb on + strandat 1707.390 kb on - strandat 1707.391 kb on + strandat 1707.404 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride
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1,704,492 - MMJJ_RS09180 0.62 -0.8
1,705,351 - -2.6
1,705,790 + MMJJ_RS09190 0.37 -1.2
1,705,995 - -0.8
1,706,038 - -0.8
1,706,081 + MMJJ_RS09190 0.62 -3.3
1,706,292 + MMJJ_RS09190 0.79 -2.0
1,707,183 - MMJJ_RS09195 0.83 +0.4
1,707,184 + MMJJ_RS09195 0.83 -1.2
1,707,209 + MMJJ_RS09195 0.86 -1.2
1,707,212 + MMJJ_RS09195 0.87 -2.7
1,707,311 - +1.4
1,707,312 + -0.4
1,707,342 + +0.3
1,707,352 + +0.7
1,707,390 - +0.3
1,707,391 + +1.6
1,707,404 - +0.6

Or see this region's nucleotide sequence