Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08220 and MMJJ_RS08225 are separated by 190 nucleotides MMJJ_RS08225 and MMJJ_RS08230 are separated by 68 nucleotides MMJJ_RS08230 and MMJJ_RS08235 are separated by 142 nucleotides MMJJ_RS08235 and MMJJ_RS08240 are separated by 80 nucleotides
MMJJ_RS08220: MMJJ_RS08220 - methenyltetrahydromethanopterin cyclohydrolase, at 1,513,835 to 1,514,806
_RS08220
MMJJ_RS08225: MMJJ_RS08225 - peptidylprolyl isomerase, at 1,514,997 to 1,515,449
_RS08225
MMJJ_RS08230: MMJJ_RS08230 - ribose-5-phosphate isomerase RpiA, at 1,515,518 to 1,516,237
_RS08230
MMJJ_RS08235: MMJJ_RS08235 - UPF0147 family protein, at 1,516,380 to 1,516,655
_RS08235
MMJJ_RS08240: MMJJ_RS08240 - L-fuculose phosphate aldolase, at 1,516,736 to 1,517,278
_RS08240
Position (kb)
1515
1516
1517 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1514.681 kb on - strand at 1514.708 kb on + strand, within MMJJ_RS08220 at 1514.725 kb on - strand at 1514.730 kb on + strand at 1514.911 kb on + strand at 1514.911 kb on + strand at 1514.915 kb on - strand at 1514.915 kb on - strand at 1514.916 kb on + strand at 1514.916 kb on + strand at 1514.918 kb on + strand at 1514.918 kb on + strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.933 kb on - strand at 1514.938 kb on - strand at 1514.939 kb on + strand at 1514.939 kb on + strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.940 kb on - strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.941 kb on + strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.942 kb on - strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.943 kb on + strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.944 kb on - strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.945 kb on + strand at 1514.947 kb on - strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.948 kb on + strand at 1514.982 kb on - strand at 1514.982 kb on - strand at 1515.014 kb on - strand at 1515.015 kb on + strand at 1515.020 kb on + strand at 1515.020 kb on + strand at 1515.030 kb on - strand at 1515.066 kb on + strand, within MMJJ_RS08225 at 1515.120 kb on - strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.121 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.131 kb on + strand, within MMJJ_RS08225 at 1515.138 kb on + strand, within MMJJ_RS08225 at 1515.355 kb on - strand, within MMJJ_RS08225 at 1515.362 kb on - strand, within MMJJ_RS08225 at 1515.435 kb on + strand at 1515.436 kb on - strand at 1515.437 kb on + strand at 1515.462 kb on + strand at 1515.470 kb on - strand at 1515.470 kb on - strand at 1515.489 kb on - strand at 1515.498 kb on + strand at 1515.498 kb on + strand at 1515.524 kb on + strand at 1515.550 kb on + strand at 1515.699 kb on + strand, within MMJJ_RS08230 at 1515.700 kb on - strand, within MMJJ_RS08230 at 1515.715 kb on - strand, within MMJJ_RS08230 at 1515.716 kb on + strand, within MMJJ_RS08230 at 1515.802 kb on + strand, within MMJJ_RS08230 at 1515.986 kb on - strand, within MMJJ_RS08230 at 1516.181 kb on + strand at 1516.225 kb on + strand at 1516.250 kb on - strand at 1516.254 kb on - strand at 1516.283 kb on - strand at 1516.283 kb on - strand at 1516.283 kb on - strand at 1516.284 kb on + strand at 1516.284 kb on + strand at 1516.296 kb on + strand at 1516.317 kb on - strand at 1516.317 kb on - strand at 1516.318 kb on + strand at 1516.318 kb on + strand at 1516.327 kb on - strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.328 kb on + strand at 1516.350 kb on - strand at 1516.354 kb on - strand at 1516.355 kb on + strand at 1516.458 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.511 kb on + strand, within MMJJ_RS08235 at 1516.590 kb on + strand, within MMJJ_RS08235 at 1516.653 kb on - strand at 1516.657 kb on - strand at 1516.672 kb on - strand at 1516.714 kb on - strand at 1516.715 kb on + strand at 1516.722 kb on - strand at 1516.754 kb on + strand at 1516.756 kb on - strand at 1516.756 kb on - strand at 1516.757 kb on + strand at 1516.758 kb on - strand at 1516.759 kb on + strand at 1516.759 kb on + strand at 1516.764 kb on + strand at 1516.782 kb on + strand at 1516.782 kb on + strand at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.800 kb on - strand, within MMJJ_RS08240 at 1516.801 kb on + strand, within MMJJ_RS08240 at 1516.812 kb on + strand, within MMJJ_RS08240 at 1516.813 kb on - strand, within MMJJ_RS08240 at 1516.829 kb on + strand, within MMJJ_RS08240 at 1516.853 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.915 kb on + strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.923 kb on - strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.924 kb on + strand, within MMJJ_RS08240 at 1516.928 kb on - strand, within MMJJ_RS08240 at 1516.970 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.004 kb on - strand, within MMJJ_RS08240 at 1517.005 kb on + strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.024 kb on - strand, within MMJJ_RS08240 at 1517.052 kb on - strand, within MMJJ_RS08240 at 1517.054 kb on - strand, within MMJJ_RS08240 at 1517.072 kb on - strand, within MMJJ_RS08240 at 1517.073 kb on + strand, within MMJJ_RS08240 at 1517.083 kb on - strand, within MMJJ_RS08240 at 1517.097 kb on + strand, within MMJJ_RS08240 at 1517.156 kb on - strand, within MMJJ_RS08240 at 1517.170 kb on - strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.171 kb on + strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.173 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.175 kb on - strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.176 kb on + strand, within MMJJ_RS08240 at 1517.197 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.201 kb on - strand, within MMJJ_RS08240 at 1517.202 kb on + strand, within MMJJ_RS08240 at 1517.226 kb on - strand at 1517.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,514,681 - -1.1 1,514,708 + MMJJ_RS08220 0.90 -2.8 1,514,725 - -1.0 1,514,730 + -1.8 1,514,911 + -1.0 1,514,911 + +0.4 1,514,915 - +1.0 1,514,915 - -2.4 1,514,916 + +0.6 1,514,916 + -0.3 1,514,918 + -0.6 1,514,918 + -0.6 1,514,933 - +0.2 1,514,933 - -0.6 1,514,933 - -0.9 1,514,938 - -1.1 1,514,939 + -0.6 1,514,939 + +0.2 1,514,940 - +0.2 1,514,940 - -0.7 1,514,940 - -3.1 1,514,940 - -0.6 1,514,940 - +0.5 1,514,940 - +0.0 1,514,941 + -0.2 1,514,941 + +0.3 1,514,941 + -0.4 1,514,941 + +0.5 1,514,941 + +0.8 1,514,941 + -0.2 1,514,941 + +1.4 1,514,941 + +1.5 1,514,941 + -0.6 1,514,942 - -0.6 1,514,942 - -0.4 1,514,942 - -0.6 1,514,942 - +0.5 1,514,942 - -0.3 1,514,942 - +1.2 1,514,942 - +1.2 1,514,942 - +1.2 1,514,942 - -0.2 1,514,942 - +1.0 1,514,942 - -0.9 1,514,942 - -1.6 1,514,942 - +2.0 1,514,943 + -0.7 1,514,943 + -0.5 1,514,943 + +0.5 1,514,943 + +1.2 1,514,943 + +0.2 1,514,943 + +0.4 1,514,943 + +0.2 1,514,943 + +0.3 1,514,944 - -0.6 1,514,944 - -0.5 1,514,944 - -1.2 1,514,944 - +0.6 1,514,944 - -0.7 1,514,944 - -0.4 1,514,944 - +2.2 1,514,945 + -1.2 1,514,945 + -1.1 1,514,945 + +1.9 1,514,947 - +1.6 1,514,948 + +1.3 1,514,948 + -0.2 1,514,948 + -1.1 1,514,982 - +0.5 1,514,982 - +0.6 1,515,014 - +0.7 1,515,015 + -0.7 1,515,020 + -0.8 1,515,020 + +0.7 1,515,030 - +0.9 1,515,066 + MMJJ_RS08225 0.15 +1.6 1,515,120 - MMJJ_RS08225 0.27 +0.2 1,515,121 + MMJJ_RS08225 0.27 +1.0 1,515,121 + MMJJ_RS08225 0.27 +0.1 1,515,121 + MMJJ_RS08225 0.27 -0.3 1,515,131 + MMJJ_RS08225 0.30 -2.0 1,515,131 + MMJJ_RS08225 0.30 +0.5 1,515,138 + MMJJ_RS08225 0.31 -0.7 1,515,355 - MMJJ_RS08225 0.79 -1.4 1,515,362 - MMJJ_RS08225 0.81 +0.8 1,515,435 + +2.2 1,515,436 - -0.7 1,515,437 + +2.4 1,515,462 + +1.0 1,515,470 - +0.7 1,515,470 - +1.8 1,515,489 - -0.9 1,515,498 + -0.3 1,515,498 + +1.9 1,515,524 + -2.0 1,515,550 + -1.1 1,515,699 + MMJJ_RS08230 0.25 -0.4 1,515,700 - MMJJ_RS08230 0.25 -1.7 1,515,715 - MMJJ_RS08230 0.27 -1.8 1,515,716 + MMJJ_RS08230 0.28 -1.7 1,515,802 + MMJJ_RS08230 0.39 -1.6 1,515,986 - MMJJ_RS08230 0.65 -1.8 1,516,181 + -0.8 1,516,225 + -2.2 1,516,250 - +0.2 1,516,254 - -0.6 1,516,283 - +0.1 1,516,283 - -2.3 1,516,283 - +0.8 1,516,284 + -1.4 1,516,284 + -0.5 1,516,296 + -0.2 1,516,317 - -1.6 1,516,317 - -0.6 1,516,318 + +0.1 1,516,318 + -1.4 1,516,327 - -0.8 1,516,328 + -1.2 1,516,328 + -1.3 1,516,328 + -0.9 1,516,350 - +1.4 1,516,354 - -0.7 1,516,355 + -1.4 1,516,458 + MMJJ_RS08235 0.28 -0.5 1,516,511 + MMJJ_RS08235 0.47 -1.9 1,516,511 + MMJJ_RS08235 0.47 -2.9 1,516,590 + MMJJ_RS08235 0.76 -1.8 1,516,653 - +1.4 1,516,657 - +1.6 1,516,672 - -1.2 1,516,714 - -0.6 1,516,715 + +0.1 1,516,722 - +0.6 1,516,754 + +0.6 1,516,756 - -1.2 1,516,756 - -0.8 1,516,757 + -0.4 1,516,758 - -0.4 1,516,759 + +0.3 1,516,759 + +0.6 1,516,764 + -0.5 1,516,782 + -0.5 1,516,782 + +1.1 1,516,800 - MMJJ_RS08240 0.12 +0.9 1,516,800 - MMJJ_RS08240 0.12 +0.5 1,516,801 + MMJJ_RS08240 0.12 -0.5 1,516,812 + MMJJ_RS08240 0.14 +1.0 1,516,813 - MMJJ_RS08240 0.14 +0.8 1,516,829 + MMJJ_RS08240 0.17 -0.6 1,516,853 + MMJJ_RS08240 0.22 -0.2 1,516,915 + MMJJ_RS08240 0.33 -1.3 1,516,915 + MMJJ_RS08240 0.33 +1.4 1,516,923 - MMJJ_RS08240 0.34 -0.4 1,516,923 - MMJJ_RS08240 0.34 +0.9 1,516,924 + MMJJ_RS08240 0.35 -1.1 1,516,924 + MMJJ_RS08240 0.35 +0.9 1,516,924 + MMJJ_RS08240 0.35 +1.2 1,516,924 + MMJJ_RS08240 0.35 -0.6 1,516,924 + MMJJ_RS08240 0.35 -1.3 1,516,924 + MMJJ_RS08240 0.35 +0.7 1,516,928 - MMJJ_RS08240 0.35 -0.6 1,516,970 - MMJJ_RS08240 0.43 +0.8 1,517,004 - MMJJ_RS08240 0.49 -1.1 1,517,004 - MMJJ_RS08240 0.49 -2.6 1,517,005 + MMJJ_RS08240 0.50 +1.3 1,517,024 - MMJJ_RS08240 0.53 -0.2 1,517,024 - MMJJ_RS08240 0.53 -1.6 1,517,052 - MMJJ_RS08240 0.58 +0.6 1,517,054 - MMJJ_RS08240 0.59 -0.0 1,517,072 - MMJJ_RS08240 0.62 -0.3 1,517,073 + MMJJ_RS08240 0.62 -2.4 1,517,083 - MMJJ_RS08240 0.64 -1.1 1,517,097 + MMJJ_RS08240 0.66 +0.8 1,517,156 - MMJJ_RS08240 0.77 +0.8 1,517,170 - MMJJ_RS08240 0.80 +1.1 1,517,171 + MMJJ_RS08240 0.80 -1.2 1,517,171 + MMJJ_RS08240 0.80 +1.1 1,517,173 - MMJJ_RS08240 0.80 +0.2 1,517,173 - MMJJ_RS08240 0.80 -0.6 1,517,173 - MMJJ_RS08240 0.80 -0.9 1,517,175 - MMJJ_RS08240 0.81 -0.3 1,517,175 - MMJJ_RS08240 0.81 +0.0 1,517,175 - MMJJ_RS08240 0.81 +0.2 1,517,175 - MMJJ_RS08240 0.81 +1.6 1,517,176 + MMJJ_RS08240 0.81 +1.7 1,517,176 + MMJJ_RS08240 0.81 -0.5 1,517,176 + MMJJ_RS08240 0.81 -0.8 1,517,176 + MMJJ_RS08240 0.81 -0.7 1,517,176 + MMJJ_RS08240 0.81 -0.5 1,517,197 - MMJJ_RS08240 0.85 -2.1 1,517,201 - MMJJ_RS08240 0.86 +1.0 1,517,201 - MMJJ_RS08240 0.86 +1.8 1,517,202 + MMJJ_RS08240 0.86 +0.8 1,517,226 - -0.9 1,517,226 - -0.2
Or see this region's nucleotide sequence