Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08090 and MMJJ_RS08095 are separated by 123 nucleotides MMJJ_RS08095 and MMJJ_RS08100 are separated by 98 nucleotides MMJJ_RS08100 and MMJJ_RS08105 are separated by 46 nucleotides
MMJJ_RS08090: MMJJ_RS08090 - histidinol-phosphate transaminase, at 1,483,744 to 1,484,859
_RS08090
MMJJ_RS08095: MMJJ_RS08095 - cobyric acid synthase CobQ, at 1,484,983 to 1,486,461
_RS08095
MMJJ_RS08100: MMJJ_RS08100 - hypothetical protein, at 1,486,560 to 1,487,237
_RS08100
MMJJ_RS08105: MMJJ_RS08105 - Zn-ribbon domain-containing OB-fold protein, at 1,487,284 to 1,487,673
_RS08105
Position (kb)
1484
1485
1486
1487 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1484.748 kb on + strand at 1484.791 kb on + strand at 1484.857 kb on - strand at 1484.858 kb on + strand at 1484.885 kb on - strand at 1484.911 kb on - strand at 1484.912 kb on + strand at 1485.114 kb on + strand at 1485.162 kb on - strand, within MMJJ_RS08095 at 1485.300 kb on - strand, within MMJJ_RS08095 at 1485.301 kb on + strand, within MMJJ_RS08095 at 1485.365 kb on + strand, within MMJJ_RS08095 at 1485.432 kb on + strand, within MMJJ_RS08095 at 1485.465 kb on + strand, within MMJJ_RS08095 at 1485.465 kb on + strand, within MMJJ_RS08095 at 1485.586 kb on + strand, within MMJJ_RS08095 at 1485.989 kb on - strand, within MMJJ_RS08095 at 1485.990 kb on + strand, within MMJJ_RS08095 at 1486.116 kb on + strand, within MMJJ_RS08095 at 1486.120 kb on + strand, within MMJJ_RS08095 at 1486.283 kb on + strand, within MMJJ_RS08095 at 1486.356 kb on + strand at 1486.379 kb on + strand at 1486.379 kb on + strand at 1486.455 kb on + strand at 1486.460 kb on + strand at 1486.465 kb on + strand at 1486.465 kb on + strand at 1486.507 kb on + strand at 1486.523 kb on - strand at 1486.524 kb on + strand at 1486.533 kb on - strand at 1486.534 kb on + strand at 1486.559 kb on - strand at 1486.559 kb on - strand at 1486.560 kb on + strand at 1486.593 kb on - strand at 1486.593 kb on - strand at 1486.596 kb on - strand at 1486.596 kb on - strand at 1486.597 kb on + strand at 1486.597 kb on + strand at 1486.597 kb on + strand at 1486.597 kb on + strand at 1486.624 kb on + strand at 1486.624 kb on + strand at 1486.624 kb on + strand at 1486.670 kb on - strand, within MMJJ_RS08100 at 1486.670 kb on - strand, within MMJJ_RS08100 at 1486.670 kb on - strand, within MMJJ_RS08100 at 1486.671 kb on + strand, within MMJJ_RS08100 at 1486.682 kb on - strand, within MMJJ_RS08100 at 1486.717 kb on - strand, within MMJJ_RS08100 at 1486.717 kb on - strand, within MMJJ_RS08100 at 1486.770 kb on - strand, within MMJJ_RS08100 at 1486.770 kb on - strand, within MMJJ_RS08100 at 1486.770 kb on - strand, within MMJJ_RS08100 at 1486.771 kb on + strand, within MMJJ_RS08100 at 1486.771 kb on + strand, within MMJJ_RS08100 at 1486.784 kb on + strand, within MMJJ_RS08100 at 1486.859 kb on - strand, within MMJJ_RS08100 at 1486.860 kb on + strand, within MMJJ_RS08100 at 1486.860 kb on + strand, within MMJJ_RS08100 at 1486.886 kb on - strand, within MMJJ_RS08100 at 1486.920 kb on + strand, within MMJJ_RS08100 at 1486.944 kb on - strand, within MMJJ_RS08100 at 1486.945 kb on + strand, within MMJJ_RS08100 at 1486.951 kb on + strand, within MMJJ_RS08100 at 1487.094 kb on + strand, within MMJJ_RS08100 at 1487.182 kb on - strand at 1487.183 kb on + strand at 1487.184 kb on - strand at 1487.185 kb on + strand at 1487.185 kb on + strand at 1487.203 kb on - strand at 1487.204 kb on + strand at 1487.215 kb on - strand at 1487.235 kb on - strand at 1487.236 kb on + strand at 1487.278 kb on - strand at 1487.278 kb on - strand at 1487.279 kb on + strand at 1487.279 kb on + strand at 1487.283 kb on + strand at 1487.452 kb on - strand, within MMJJ_RS08105
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,484,748 + -3.5 1,484,791 + -1.8 1,484,857 - +0.6 1,484,858 + +1.3 1,484,885 - -0.1 1,484,911 - -0.4 1,484,912 + -2.7 1,485,114 + -2.3 1,485,162 - MMJJ_RS08095 0.12 -1.4 1,485,300 - MMJJ_RS08095 0.21 -3.4 1,485,301 + MMJJ_RS08095 0.22 -2.5 1,485,365 + MMJJ_RS08095 0.26 -3.1 1,485,432 + MMJJ_RS08095 0.30 -2.1 1,485,465 + MMJJ_RS08095 0.33 -1.4 1,485,465 + MMJJ_RS08095 0.33 -2.0 1,485,586 + MMJJ_RS08095 0.41 -2.8 1,485,989 - MMJJ_RS08095 0.68 -2.1 1,485,990 + MMJJ_RS08095 0.68 -2.7 1,486,116 + MMJJ_RS08095 0.77 -2.0 1,486,120 + MMJJ_RS08095 0.77 -1.8 1,486,283 + MMJJ_RS08095 0.88 -1.8 1,486,356 + -0.4 1,486,379 + -2.5 1,486,379 + -2.8 1,486,455 + -0.2 1,486,460 + +0.5 1,486,465 + +0.8 1,486,465 + -1.1 1,486,507 + -0.8 1,486,523 - +0.8 1,486,524 + +0.2 1,486,533 - +1.0 1,486,534 + +0.2 1,486,559 - -0.8 1,486,559 - +1.0 1,486,560 + +0.1 1,486,593 - +0.5 1,486,593 - -1.9 1,486,596 - +1.6 1,486,596 - -1.1 1,486,597 + -0.8 1,486,597 + +0.8 1,486,597 + +0.6 1,486,597 + -0.7 1,486,624 + -1.1 1,486,624 + +0.4 1,486,624 + -0.4 1,486,670 - MMJJ_RS08100 0.16 +0.8 1,486,670 - MMJJ_RS08100 0.16 +0.1 1,486,670 - MMJJ_RS08100 0.16 +0.8 1,486,671 + MMJJ_RS08100 0.16 -1.0 1,486,682 - MMJJ_RS08100 0.18 -0.4 1,486,717 - MMJJ_RS08100 0.23 +0.2 1,486,717 - MMJJ_RS08100 0.23 +0.5 1,486,770 - MMJJ_RS08100 0.31 -0.3 1,486,770 - MMJJ_RS08100 0.31 -1.6 1,486,770 - MMJJ_RS08100 0.31 -0.0 1,486,771 + MMJJ_RS08100 0.31 +0.0 1,486,771 + MMJJ_RS08100 0.31 -1.2 1,486,784 + MMJJ_RS08100 0.33 +0.3 1,486,859 - MMJJ_RS08100 0.44 +0.5 1,486,860 + MMJJ_RS08100 0.44 -0.1 1,486,860 + MMJJ_RS08100 0.44 +1.0 1,486,886 - MMJJ_RS08100 0.48 +0.2 1,486,920 + MMJJ_RS08100 0.53 -2.3 1,486,944 - MMJJ_RS08100 0.57 +0.5 1,486,945 + MMJJ_RS08100 0.57 -0.1 1,486,951 + MMJJ_RS08100 0.58 -0.1 1,487,094 + MMJJ_RS08100 0.79 -0.3 1,487,182 - -0.1 1,487,183 + +0.3 1,487,184 - +0.2 1,487,185 + +1.0 1,487,185 + +0.8 1,487,203 - +0.7 1,487,204 + +0.7 1,487,215 - +0.3 1,487,235 - -0.6 1,487,236 + -2.0 1,487,278 - -0.4 1,487,278 - -1.1 1,487,279 + -0.8 1,487,279 + +0.6 1,487,283 + +1.2 1,487,452 - MMJJ_RS08105 0.43 -2.3
Or see this region's nucleotide sequence