Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07870 and MMJJ_RS07875 are separated by 227 nucleotides MMJJ_RS07875 and MMJJ_RS07880 are separated by 142 nucleotides
MMJJ_RS07870: MMJJ_RS07870 - ABC transporter substrate-binding protein, at 1,443,233 to 1,444,381
_RS07870
MMJJ_RS07875: MMJJ_RS07875 - FAD-dependent oxidoreductase, at 1,444,609 to 1,445,937
_RS07875
MMJJ_RS07880: MMJJ_RS07880 - porphobilinogen synthase, at 1,446,080 to 1,447,048
_RS07880
Position (kb)
1444
1445
1446 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1443.705 kb on + strand, within MMJJ_RS07870 at 1443.773 kb on - strand, within MMJJ_RS07870 at 1443.774 kb on + strand, within MMJJ_RS07870 at 1443.779 kb on + strand, within MMJJ_RS07870 at 1443.811 kb on - strand at 1443.812 kb on + strand, within MMJJ_RS07870 at 1443.812 kb on + strand, within MMJJ_RS07870 at 1443.815 kb on - strand, within MMJJ_RS07870 at 1443.816 kb on + strand, within MMJJ_RS07870 at 1443.841 kb on - strand, within MMJJ_RS07870 at 1443.841 kb on - strand, within MMJJ_RS07870 at 1443.841 kb on - strand, within MMJJ_RS07870 at 1443.841 kb on - strand, within MMJJ_RS07870 at 1443.841 kb on - strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.842 kb on + strand, within MMJJ_RS07870 at 1443.891 kb on - strand, within MMJJ_RS07870 at 1443.892 kb on + strand, within MMJJ_RS07870 at 1443.918 kb on - strand, within MMJJ_RS07870 at 1443.918 kb on - strand, within MMJJ_RS07870 at 1444.033 kb on + strand, within MMJJ_RS07870 at 1444.144 kb on + strand, within MMJJ_RS07870 at 1444.176 kb on - strand, within MMJJ_RS07870 at 1444.176 kb on - strand, within MMJJ_RS07870 at 1444.176 kb on - strand, within MMJJ_RS07870 at 1444.177 kb on + strand, within MMJJ_RS07870 at 1444.233 kb on - strand, within MMJJ_RS07870 at 1444.234 kb on + strand, within MMJJ_RS07870 at 1444.241 kb on + strand, within MMJJ_RS07870 at 1444.241 kb on + strand, within MMJJ_RS07870 at 1444.350 kb on - strand at 1444.350 kb on - strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.351 kb on + strand at 1444.352 kb on - strand at 1444.352 kb on - strand at 1444.352 kb on - strand at 1444.353 kb on + strand at 1444.354 kb on - strand at 1444.354 kb on - strand at 1444.411 kb on - strand at 1444.411 kb on - strand at 1444.419 kb on + strand at 1444.448 kb on + strand at 1444.532 kb on + strand at 1444.553 kb on + strand at 1444.555 kb on + strand at 1444.749 kb on + strand, within MMJJ_RS07875 at 1444.928 kb on - strand, within MMJJ_RS07875 at 1445.313 kb on - strand, within MMJJ_RS07875 at 1445.328 kb on - strand, within MMJJ_RS07875 at 1445.462 kb on - strand, within MMJJ_RS07875 at 1445.526 kb on - strand, within MMJJ_RS07875 at 1445.946 kb on + strand at 1445.998 kb on - strand at 1445.999 kb on + strand at 1446.004 kb on + strand at 1446.004 kb on + strand at 1446.017 kb on + strand at 1446.017 kb on + strand at 1446.037 kb on + strand at 1446.037 kb on + strand at 1446.037 kb on + strand at 1446.038 kb on - strand at 1446.039 kb on + strand at 1446.039 kb on + strand at 1446.039 kb on + strand at 1446.042 kb on + strand at 1446.042 kb on + strand at 1446.044 kb on + strand at 1446.044 kb on + strand at 1446.218 kb on + strand, within MMJJ_RS07880 at 1446.394 kb on + strand, within MMJJ_RS07880 at 1446.421 kb on + strand, within MMJJ_RS07880
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,443,705 + MMJJ_RS07870 0.41 -0.8 1,443,773 - MMJJ_RS07870 0.47 -2.9 1,443,774 + MMJJ_RS07870 0.47 -3.4 1,443,779 + MMJJ_RS07870 0.48 -2.0 1,443,811 - +0.8 1,443,812 + MMJJ_RS07870 0.50 +0.7 1,443,812 + MMJJ_RS07870 0.50 -0.7 1,443,815 - MMJJ_RS07870 0.51 -3.1 1,443,816 + MMJJ_RS07870 0.51 -4.2 1,443,841 - MMJJ_RS07870 0.53 -1.1 1,443,841 - MMJJ_RS07870 0.53 -2.0 1,443,841 - MMJJ_RS07870 0.53 +0.9 1,443,841 - MMJJ_RS07870 0.53 -3.5 1,443,841 - MMJJ_RS07870 0.53 -2.2 1,443,842 + MMJJ_RS07870 0.53 -1.7 1,443,842 + MMJJ_RS07870 0.53 -2.0 1,443,842 + MMJJ_RS07870 0.53 -2.2 1,443,842 + MMJJ_RS07870 0.53 -1.1 1,443,842 + MMJJ_RS07870 0.53 -2.7 1,443,842 + MMJJ_RS07870 0.53 -1.6 1,443,891 - MMJJ_RS07870 0.57 -2.0 1,443,892 + MMJJ_RS07870 0.57 -1.1 1,443,918 - MMJJ_RS07870 0.60 -0.4 1,443,918 - MMJJ_RS07870 0.60 -0.6 1,444,033 + MMJJ_RS07870 0.70 -0.3 1,444,144 + MMJJ_RS07870 0.79 -1.2 1,444,176 - MMJJ_RS07870 0.82 -1.6 1,444,176 - MMJJ_RS07870 0.82 -2.0 1,444,176 - MMJJ_RS07870 0.82 -1.3 1,444,177 + MMJJ_RS07870 0.82 -0.8 1,444,233 - MMJJ_RS07870 0.87 -1.1 1,444,234 + MMJJ_RS07870 0.87 -1.8 1,444,241 + MMJJ_RS07870 0.88 -1.9 1,444,241 + MMJJ_RS07870 0.88 -1.9 1,444,350 - +0.7 1,444,350 - -0.3 1,444,351 + -0.7 1,444,351 + +0.2 1,444,351 + +1.2 1,444,351 + -0.1 1,444,351 + -0.5 1,444,351 + -1.0 1,444,351 + +0.6 1,444,351 + +1.2 1,444,352 - +0.7 1,444,352 - -2.3 1,444,352 - -0.1 1,444,353 + +0.1 1,444,354 - -0.3 1,444,354 - +0.4 1,444,411 - -1.0 1,444,411 - +1.0 1,444,419 + +0.3 1,444,448 + +0.5 1,444,532 + -0.3 1,444,553 + -1.1 1,444,555 + -1.9 1,444,749 + MMJJ_RS07875 0.11 -1.1 1,444,928 - MMJJ_RS07875 0.24 -2.8 1,445,313 - MMJJ_RS07875 0.53 +1.2 1,445,328 - MMJJ_RS07875 0.54 -1.6 1,445,462 - MMJJ_RS07875 0.64 -1.3 1,445,526 - MMJJ_RS07875 0.69 -0.8 1,445,946 + +0.4 1,445,998 - -1.6 1,445,999 + +0.4 1,446,004 + -1.6 1,446,004 + -2.8 1,446,017 + -1.2 1,446,017 + -0.2 1,446,037 + -0.6 1,446,037 + -2.1 1,446,037 + -2.3 1,446,038 - -2.0 1,446,039 + -3.6 1,446,039 + -2.0 1,446,039 + -1.8 1,446,042 + -2.3 1,446,042 + -2.6 1,446,044 + -2.9 1,446,044 + -5.1 1,446,218 + MMJJ_RS07880 0.14 -2.7 1,446,394 + MMJJ_RS07880 0.32 -2.2 1,446,421 + MMJJ_RS07880 0.35 -3.1
Or see this region's nucleotide sequence