Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07180 and MMJJ_RS07185 are separated by 2 nucleotides MMJJ_RS07185 and MMJJ_RS07190 are separated by 138 nucleotides MMJJ_RS07190 and MMJJ_RS07195 are separated by 78 nucleotides
MMJJ_RS07180: MMJJ_RS07180 - type I 3-dehydroquinate dehydratase, at 1,314,454 to 1,315,110
_RS07180
MMJJ_RS07185: MMJJ_RS07185 - DUF371 domain-containing protein, at 1,315,113 to 1,315,529
_RS07185
MMJJ_RS07190: MMJJ_RS07190 - RtcB family protein, at 1,315,668 to 1,317,110
_RS07190
MMJJ_RS07195: MMJJ_RS07195 - aspartate-semialdehyde dehydrogenase, at 1,317,189 to 1,318,235
_RS07195
Position (kb)
1315
1316
1317
1318 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1315.103 kb on - strand at 1315.108 kb on - strand at 1315.109 kb on + strand at 1315.224 kb on + strand, within MMJJ_RS07185 at 1315.391 kb on - strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185 at 1315.392 kb on + strand, within MMJJ_RS07185 at 1315.454 kb on - strand, within MMJJ_RS07185 at 1315.530 kb on - strand at 1315.566 kb on - strand at 1315.566 kb on - strand at 1315.567 kb on + strand at 1315.576 kb on - strand at 1315.592 kb on - strand at 1315.596 kb on - strand at 1315.596 kb on - strand at 1315.603 kb on + strand at 1315.603 kb on + strand at 1315.611 kb on + strand at 1315.611 kb on + strand at 1315.614 kb on - strand at 1315.614 kb on - strand at 1315.615 kb on + strand at 1315.615 kb on + strand at 1315.618 kb on - strand at 1315.621 kb on + strand at 1315.796 kb on + strand at 1315.799 kb on - strand at 1315.925 kb on + strand, within MMJJ_RS07190 at 1316.260 kb on - strand, within MMJJ_RS07190 at 1316.305 kb on - strand, within MMJJ_RS07190 at 1316.419 kb on + strand, within MMJJ_RS07190 at 1316.419 kb on + strand, within MMJJ_RS07190 at 1316.419 kb on + strand, within MMJJ_RS07190 at 1316.451 kb on + strand, within MMJJ_RS07190 at 1316.539 kb on + strand at 1316.539 kb on + strand, within MMJJ_RS07190 at 1316.632 kb on - strand, within MMJJ_RS07190 at 1316.636 kb on + strand, within MMJJ_RS07190 at 1316.636 kb on + strand, within MMJJ_RS07190 at 1316.638 kb on + strand, within MMJJ_RS07190 at 1316.645 kb on + strand, within MMJJ_RS07190 at 1317.042 kb on + strand at 1317.044 kb on + strand at 1317.044 kb on + strand at 1317.120 kb on + strand at 1317.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,315,103 - +1.0 1,315,108 - +1.1 1,315,109 + +1.8 1,315,224 + MMJJ_RS07185 0.27 -0.4 1,315,391 - MMJJ_RS07185 0.67 -0.3 1,315,392 + MMJJ_RS07185 0.67 -0.8 1,315,392 + MMJJ_RS07185 0.67 +1.4 1,315,454 - MMJJ_RS07185 0.82 -0.6 1,315,530 - +0.7 1,315,566 - +1.1 1,315,566 - +1.0 1,315,567 + -0.6 1,315,576 - -2.5 1,315,592 - -1.9 1,315,596 - -0.8 1,315,596 - -1.2 1,315,603 + +0.6 1,315,603 + -3.4 1,315,611 + -1.0 1,315,611 + -0.1 1,315,614 - -1.4 1,315,614 - -2.1 1,315,615 + -0.7 1,315,615 + -2.7 1,315,618 - +2.4 1,315,621 + -0.6 1,315,796 + -2.7 1,315,799 - -1.8 1,315,925 + MMJJ_RS07190 0.18 -1.8 1,316,260 - MMJJ_RS07190 0.41 -2.8 1,316,305 - MMJJ_RS07190 0.44 -2.4 1,316,419 + MMJJ_RS07190 0.52 -1.9 1,316,419 + MMJJ_RS07190 0.52 -1.6 1,316,419 + MMJJ_RS07190 0.52 -1.4 1,316,451 + MMJJ_RS07190 0.54 -2.8 1,316,539 + -0.9 1,316,539 + MMJJ_RS07190 0.60 -0.6 1,316,632 - MMJJ_RS07190 0.67 -0.6 1,316,636 + MMJJ_RS07190 0.67 -1.6 1,316,636 + MMJJ_RS07190 0.67 -2.7 1,316,638 + MMJJ_RS07190 0.67 -3.2 1,316,645 + MMJJ_RS07190 0.68 -1.2 1,317,042 + -0.6 1,317,044 + -2.6 1,317,044 + -1.4 1,317,120 + -1.9 1,317,701 + -1.2
Or see this region's nucleotide sequence