Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07000 and MMJJ_RS07005 are separated by 71 nucleotides MMJJ_RS07005 and MMJJ_RS07010 are separated by 54 nucleotides MMJJ_RS07010 and MMJJ_RS07015 are separated by 72 nucleotides
MMJJ_RS07000: MMJJ_RS07000 - TrkH family potassium uptake protein, at 1,285,117 to 1,286,553
_RS07000
MMJJ_RS07005: MMJJ_RS07005 - transcription factor S, at 1,286,625 to 1,286,942
_RS07005
MMJJ_RS07010: MMJJ_RS07010 - preprotein translocase subunit Sec61beta, at 1,286,997 to 1,287,158
_RS07010
MMJJ_RS07015: MMJJ_RS07015 - PFL family protein, at 1,287,231 to 1,288,607
_RS07015
Position (kb)
1286
1287 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1285.669 kb on - strand, within MMJJ_RS07000 at 1285.904 kb on - strand, within MMJJ_RS07000 at 1286.058 kb on + strand at 1286.265 kb on + strand, within MMJJ_RS07000 at 1286.278 kb on + strand at 1286.278 kb on + strand, within MMJJ_RS07000 at 1286.480 kb on - strand at 1286.498 kb on + strand at 1286.521 kb on + strand at 1286.550 kb on + strand at 1286.551 kb on - strand at 1286.551 kb on - strand at 1286.552 kb on + strand at 1286.556 kb on + strand at 1286.582 kb on - strand at 1286.590 kb on - strand at 1286.590 kb on - strand at 1286.590 kb on - strand at 1286.591 kb on + strand at 1286.591 kb on + strand at 1286.593 kb on + strand at 1286.600 kb on + strand at 1286.602 kb on + strand at 1286.602 kb on + strand at 1286.624 kb on - strand at 1286.624 kb on - strand at 1286.624 kb on - strand at 1286.625 kb on + strand at 1286.642 kb on - strand at 1286.654 kb on - strand at 1286.734 kb on + strand, within MMJJ_RS07005 at 1286.741 kb on + strand, within MMJJ_RS07005 at 1286.741 kb on + strand, within MMJJ_RS07005 at 1286.774 kb on - strand, within MMJJ_RS07005 at 1286.792 kb on + strand, within MMJJ_RS07005 at 1286.815 kb on - strand, within MMJJ_RS07005 at 1286.815 kb on - strand, within MMJJ_RS07005 at 1286.857 kb on - strand, within MMJJ_RS07005 at 1286.899 kb on + strand, within MMJJ_RS07005 at 1286.921 kb on - strand at 1286.934 kb on - strand at 1286.945 kb on + strand at 1286.962 kb on - strand at 1287.037 kb on + strand, within MMJJ_RS07010 at 1287.038 kb on - strand, within MMJJ_RS07010 at 1287.039 kb on + strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.104 kb on - strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.105 kb on + strand, within MMJJ_RS07010 at 1287.106 kb on - strand, within MMJJ_RS07010 at 1287.106 kb on - strand, within MMJJ_RS07010 at 1287.106 kb on - strand at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.107 kb on + strand, within MMJJ_RS07010 at 1287.111 kb on - strand, within MMJJ_RS07010 at 1287.112 kb on + strand, within MMJJ_RS07010 at 1287.112 kb on + strand, within MMJJ_RS07010 at 1287.153 kb on + strand at 1287.169 kb on - strand at 1287.169 kb on - strand at 1287.180 kb on - strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.181 kb on + strand at 1287.182 kb on - strand at 1287.182 kb on - strand at 1287.182 kb on - strand at 1287.183 kb on + strand at 1287.183 kb on + strand at 1287.224 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,285,669 - MMJJ_RS07000 0.38 -2.3 1,285,904 - MMJJ_RS07000 0.55 -1.2 1,286,058 + +1.4 1,286,265 + MMJJ_RS07000 0.80 -1.1 1,286,278 + -0.6 1,286,278 + MMJJ_RS07000 0.81 -0.8 1,286,480 - +0.3 1,286,498 + -3.4 1,286,521 + +1.4 1,286,550 + -1.4 1,286,551 - +0.2 1,286,551 - -1.2 1,286,552 + -1.3 1,286,556 + -0.0 1,286,582 - -1.9 1,286,590 - -0.2 1,286,590 - -1.3 1,286,590 - -4.1 1,286,591 + -1.5 1,286,591 + -0.3 1,286,593 + -1.4 1,286,600 + +0.1 1,286,602 + -2.9 1,286,602 + -0.9 1,286,624 - -1.2 1,286,624 - -2.0 1,286,624 - -1.6 1,286,625 + -2.3 1,286,642 - -1.3 1,286,654 - -0.1 1,286,734 + MMJJ_RS07005 0.34 -3.8 1,286,741 + MMJJ_RS07005 0.36 -2.0 1,286,741 + MMJJ_RS07005 0.36 -2.0 1,286,774 - MMJJ_RS07005 0.47 -1.3 1,286,792 + MMJJ_RS07005 0.53 -1.0 1,286,815 - MMJJ_RS07005 0.60 -1.2 1,286,815 - MMJJ_RS07005 0.60 -2.3 1,286,857 - MMJJ_RS07005 0.73 -3.1 1,286,899 + MMJJ_RS07005 0.86 -3.6 1,286,921 - +0.1 1,286,934 - +0.6 1,286,945 + -0.1 1,286,962 - +0.2 1,287,037 + MMJJ_RS07010 0.25 +0.8 1,287,038 - MMJJ_RS07010 0.25 -1.1 1,287,039 + MMJJ_RS07010 0.26 +0.0 1,287,104 - MMJJ_RS07010 0.66 +0.8 1,287,104 - MMJJ_RS07010 0.66 -1.3 1,287,104 - MMJJ_RS07010 0.66 +0.3 1,287,104 - MMJJ_RS07010 0.66 -1.4 1,287,105 + MMJJ_RS07010 0.67 -0.9 1,287,105 + MMJJ_RS07010 0.67 +0.3 1,287,105 + MMJJ_RS07010 0.67 +1.4 1,287,105 + MMJJ_RS07010 0.67 -1.3 1,287,106 - MMJJ_RS07010 0.67 -0.0 1,287,106 - MMJJ_RS07010 0.67 +0.8 1,287,106 - +0.9 1,287,107 + MMJJ_RS07010 0.68 +0.6 1,287,107 + MMJJ_RS07010 0.68 +0.9 1,287,107 + MMJJ_RS07010 0.68 +0.2 1,287,107 + MMJJ_RS07010 0.68 -0.2 1,287,111 - MMJJ_RS07010 0.70 +0.2 1,287,112 + MMJJ_RS07010 0.71 +0.1 1,287,112 + MMJJ_RS07010 0.71 +0.0 1,287,153 + +0.7 1,287,169 - +0.4 1,287,169 - -0.8 1,287,180 - +0.4 1,287,181 + -4.1 1,287,181 + +0.2 1,287,181 + +0.7 1,287,181 + +0.4 1,287,182 - -2.0 1,287,182 - +0.9 1,287,182 - +0.4 1,287,183 + -1.7 1,287,183 + +0.6 1,287,224 + -0.4
Or see this region's nucleotide sequence