Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS09355 and MMJJ_RS06260 are separated by 185 nucleotides MMJJ_RS06260 and MMJJ_RS06265 are separated by 8 nucleotides MMJJ_RS06265 and MMJJ_RS06270 are separated by 62 nucleotides MMJJ_RS06270 and MMJJ_RS06275 are separated by 11 nucleotides
MMJJ_RS09355: MMJJ_RS09355 - hypothetical protein, at 1,162,281 to 1,162,436
_RS09355
MMJJ_RS06260: MMJJ_RS06260 - 6-carboxyhexanoate--CoA ligase, at 1,162,622 to 1,163,356
_RS06260
MMJJ_RS06265: MMJJ_RS06265 - 8-amino-7-oxononanoate synthase, at 1,163,365 to 1,164,477
_RS06265
MMJJ_RS06270: MMJJ_RS06270 - dethiobiotin synthase, at 1,164,540 to 1,165,112
_RS06270
MMJJ_RS06275: MMJJ_RS06275 - hypothetical protein, at 1,165,124 to 1,165,420
_RS06275
Position (kb) 1163
1164
1165 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.367 kb on - strand, within MMJJ_RS09355 at 1162.432 kb on + strand at 1162.476 kb on - strand at 1162.478 kb on - strand at 1162.479 kb on + strand at 1162.480 kb on - strand at 1162.480 kb on - strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.481 kb on + strand at 1162.482 kb on - strand at 1162.482 kb on - strand at 1162.483 kb on + strand at 1162.483 kb on + strand at 1162.499 kb on - strand at 1162.572 kb on + strand at 1162.591 kb on - strand at 1162.592 kb on + strand at 1162.617 kb on + strand at 1162.765 kb on - strand, within MMJJ_RS06260 at 1162.800 kb on - strand, within MMJJ_RS06260 at 1162.805 kb on - strand, within MMJJ_RS06260 at 1162.876 kb on - strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.877 kb on + strand, within MMJJ_RS06260 at 1162.960 kb on - strand, within MMJJ_RS06260 at 1163.080 kb on + strand, within MMJJ_RS06260 at 1163.182 kb on - strand, within MMJJ_RS06260 at 1163.201 kb on - strand, within MMJJ_RS06260 at 1163.213 kb on - strand, within MMJJ_RS06260 at 1163.214 kb on + strand, within MMJJ_RS06260 at 1163.225 kb on + strand, within MMJJ_RS06260 at 1163.239 kb on - strand, within MMJJ_RS06260 at 1163.240 kb on + strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.247 kb on - strand, within MMJJ_RS06260 at 1163.284 kb on + strand at 1163.335 kb on - strand at 1163.351 kb on - strand at 1163.352 kb on + strand at 1163.352 kb on + strand at 1163.354 kb on - strand at 1163.355 kb on + strand at 1163.365 kb on + strand at 1163.506 kb on + strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.512 kb on - strand, within MMJJ_RS06265 at 1163.513 kb on + strand, within MMJJ_RS06265 at 1163.531 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.574 kb on + strand, within MMJJ_RS06265 at 1163.635 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.660 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.667 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.669 kb on - strand, within MMJJ_RS06265 at 1163.670 kb on + strand, within MMJJ_RS06265 at 1163.683 kb on + strand, within MMJJ_RS06265 at 1163.714 kb on - strand, within MMJJ_RS06265 at 1163.730 kb on + strand, within MMJJ_RS06265 at 1163.742 kb on + strand, within MMJJ_RS06265 at 1163.782 kb on + strand, within MMJJ_RS06265 at 1163.798 kb on - strand, within MMJJ_RS06265 at 1163.799 kb on + strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.843 kb on - strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.844 kb on + strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.914 kb on - strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.928 kb on + strand, within MMJJ_RS06265 at 1163.961 kb on + strand, within MMJJ_RS06265 at 1164.013 kb on + strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.065 kb on - strand, within MMJJ_RS06265 at 1164.066 kb on + strand, within MMJJ_RS06265 at 1164.092 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.118 kb on + strand, within MMJJ_RS06265 at 1164.160 kb on + strand, within MMJJ_RS06265 at 1164.274 kb on + strand, within MMJJ_RS06265 at 1164.375 kb on - strand at 1164.420 kb on - strand at 1164.439 kb on - strand at 1164.480 kb on - strand at 1164.481 kb on + strand at 1164.487 kb on - strand at 1164.488 kb on + strand at 1164.525 kb on - strand at 1164.526 kb on + strand at 1164.526 kb on + strand at 1164.568 kb on - strand at 1164.569 kb on + strand at 1164.569 kb on + strand at 1164.581 kb on - strand at 1164.581 kb on - strand at 1164.582 kb on + strand at 1164.582 kb on + strand at 1164.584 kb on + strand at 1164.584 kb on + strand at 1164.615 kb on + strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.621 kb on - strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.622 kb on + strand, within MMJJ_RS06270 at 1164.627 kb on + strand, within MMJJ_RS06270 at 1164.731 kb on + strand, within MMJJ_RS06270 at 1164.784 kb on + strand, within MMJJ_RS06270 at 1164.788 kb on + strand, within MMJJ_RS06270 at 1164.834 kb on - strand, within MMJJ_RS06270 at 1164.850 kb on - strand, within MMJJ_RS06270 at 1164.919 kb on - strand, within MMJJ_RS06270 at 1164.919 kb on - strand, within MMJJ_RS06270 at 1164.933 kb on - strand, within MMJJ_RS06270 at 1164.945 kb on + strand, within MMJJ_RS06270 at 1164.958 kb on - strand, within MMJJ_RS06270 at 1164.959 kb on + strand, within MMJJ_RS06270 at 1164.974 kb on + strand, within MMJJ_RS06270 at 1165.005 kb on - strand, within MMJJ_RS06270 at 1165.009 kb on + strand, within MMJJ_RS06270 at 1165.094 kb on - strand at 1165.094 kb on - strand at 1165.095 kb on + strand at 1165.095 kb on + strand at 1165.155 kb on + strand, within MMJJ_RS06275 at 1165.171 kb on - strand, within MMJJ_RS06275 at 1165.175 kb on - strand, within MMJJ_RS06275 at 1165.210 kb on - strand, within MMJJ_RS06275 at 1165.363 kb on - strand, within MMJJ_RS06275 at 1165.363 kb on - strand, within MMJJ_RS06275 at 1165.364 kb on + strand, within MMJJ_RS06275 at 1165.367 kb on - strand, within MMJJ_RS06275 at 1165.367 kb on - strand, within MMJJ_RS06275 at 1165.368 kb on + strand, within MMJJ_RS06275 at 1165.374 kb on + strand, within MMJJ_RS06275 at 1165.394 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.440 kb on - strand at 1165.441 kb on + strand at 1165.441 kb on + strand at 1165.442 kb on - strand at 1165.442 kb on - strand at 1165.443 kb on + strand at 1165.443 kb on + strand at 1165.448 kb on - strand at 1165.448 kb on - strand at 1165.449 kb on + strand at 1165.449 kb on + strand at 1165.449 kb on + strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.450 kb on - strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.451 kb on + strand at 1165.452 kb on - strand at 1165.452 kb on - strand at 1165.452 kb on - strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.453 kb on + strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.454 kb on - strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.455 kb on + strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand at 1165.458 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,162,367 - MMJJ_RS09355 0.55 +0.9 1,162,367 - MMJJ_RS09355 0.55 +0.4 1,162,432 + +1.4 1,162,476 - -0.0 1,162,478 - -0.7 1,162,479 + +0.1 1,162,480 - -1.1 1,162,480 - -0.6 1,162,481 + +0.3 1,162,481 + +1.0 1,162,481 + +1.2 1,162,481 + +0.9 1,162,482 - +1.0 1,162,482 - +1.7 1,162,483 + +0.5 1,162,483 + +0.0 1,162,499 - +1.2 1,162,572 + -0.1 1,162,591 - -0.7 1,162,592 + +1.1 1,162,617 + +0.1 1,162,765 - MMJJ_RS06260 0.19 -3.0 1,162,800 - MMJJ_RS06260 0.24 -3.0 1,162,805 - MMJJ_RS06260 0.25 -3.3 1,162,876 - MMJJ_RS06260 0.35 -1.8 1,162,877 + MMJJ_RS06260 0.35 -2.2 1,162,877 + MMJJ_RS06260 0.35 -1.2 1,162,960 - MMJJ_RS06260 0.46 -3.4 1,163,080 + MMJJ_RS06260 0.62 -1.3 1,163,182 - MMJJ_RS06260 0.76 -3.3 1,163,201 - MMJJ_RS06260 0.79 -2.3 1,163,213 - MMJJ_RS06260 0.80 -1.2 1,163,214 + MMJJ_RS06260 0.81 -1.7 1,163,225 + MMJJ_RS06260 0.82 -1.6 1,163,239 - MMJJ_RS06260 0.84 -3.0 1,163,240 + MMJJ_RS06260 0.84 -2.7 1,163,247 - MMJJ_RS06260 0.85 -1.2 1,163,247 - MMJJ_RS06260 0.85 -3.0 1,163,284 + -1.9 1,163,335 - -3.3 1,163,351 - -3.2 1,163,352 + -1.8 1,163,352 + -0.5 1,163,354 - +0.1 1,163,355 + +1.5 1,163,365 + -0.9 1,163,506 + MMJJ_RS06265 0.13 -0.4 1,163,512 - MMJJ_RS06265 0.13 -1.3 1,163,512 - MMJJ_RS06265 0.13 -2.0 1,163,512 - MMJJ_RS06265 0.13 -3.3 1,163,512 - MMJJ_RS06265 0.13 -1.6 1,163,512 - MMJJ_RS06265 0.13 -2.1 1,163,513 + MMJJ_RS06265 0.13 -1.6 1,163,531 + MMJJ_RS06265 0.15 -2.8 1,163,574 + MMJJ_RS06265 0.19 -1.2 1,163,574 + MMJJ_RS06265 0.19 -2.6 1,163,574 + MMJJ_RS06265 0.19 -1.1 1,163,635 - MMJJ_RS06265 0.24 -2.3 1,163,660 - MMJJ_RS06265 0.27 -2.1 1,163,660 - MMJJ_RS06265 0.27 -2.0 1,163,667 - MMJJ_RS06265 0.27 -3.0 1,163,667 - MMJJ_RS06265 0.27 -1.3 1,163,667 - MMJJ_RS06265 0.27 -2.0 1,163,669 - MMJJ_RS06265 0.27 +0.9 1,163,669 - MMJJ_RS06265 0.27 -1.8 1,163,669 - MMJJ_RS06265 0.27 -2.6 1,163,669 - MMJJ_RS06265 0.27 -2.1 1,163,670 + MMJJ_RS06265 0.27 -1.2 1,163,683 + MMJJ_RS06265 0.29 -2.0 1,163,714 - MMJJ_RS06265 0.31 -3.1 1,163,730 + MMJJ_RS06265 0.33 -2.3 1,163,742 + MMJJ_RS06265 0.34 -1.1 1,163,782 + MMJJ_RS06265 0.37 -3.3 1,163,798 - MMJJ_RS06265 0.39 -2.1 1,163,799 + MMJJ_RS06265 0.39 -1.4 1,163,843 - MMJJ_RS06265 0.43 -0.2 1,163,843 - MMJJ_RS06265 0.43 -2.0 1,163,843 - MMJJ_RS06265 0.43 -4.0 1,163,843 - MMJJ_RS06265 0.43 -3.4 1,163,844 + MMJJ_RS06265 0.43 -0.9 1,163,844 + MMJJ_RS06265 0.43 -2.2 1,163,844 + MMJJ_RS06265 0.43 -1.6 1,163,914 - MMJJ_RS06265 0.49 -3.5 1,163,914 - MMJJ_RS06265 0.49 -1.2 1,163,928 + MMJJ_RS06265 0.51 -1.6 1,163,928 + MMJJ_RS06265 0.51 -2.4 1,163,961 + MMJJ_RS06265 0.54 -2.1 1,164,013 + MMJJ_RS06265 0.58 -3.0 1,164,065 - MMJJ_RS06265 0.63 -1.7 1,164,065 - MMJJ_RS06265 0.63 -1.8 1,164,065 - MMJJ_RS06265 0.63 -0.7 1,164,065 - MMJJ_RS06265 0.63 -2.2 1,164,066 + MMJJ_RS06265 0.63 -1.0 1,164,092 + MMJJ_RS06265 0.65 -3.7 1,164,118 + MMJJ_RS06265 0.68 -3.0 1,164,118 + MMJJ_RS06265 0.68 -1.8 1,164,160 + MMJJ_RS06265 0.71 -2.3 1,164,274 + MMJJ_RS06265 0.82 -1.2 1,164,375 - -1.0 1,164,420 - -0.9 1,164,439 - -2.0 1,164,480 - -1.2 1,164,481 + -0.2 1,164,487 - -1.8 1,164,488 + -3.4 1,164,525 - -4.1 1,164,526 + -3.1 1,164,526 + -2.5 1,164,568 - -3.8 1,164,569 + -1.8 1,164,569 + -4.0 1,164,581 - -3.6 1,164,581 - -2.9 1,164,582 + -2.6 1,164,582 + -1.6 1,164,584 + -2.9 1,164,584 + -2.2 1,164,615 + MMJJ_RS06270 0.13 -2.2 1,164,621 - MMJJ_RS06270 0.14 -0.9 1,164,621 - MMJJ_RS06270 0.14 -2.6 1,164,621 - MMJJ_RS06270 0.14 -2.8 1,164,621 - MMJJ_RS06270 0.14 -1.6 1,164,622 + MMJJ_RS06270 0.14 -2.1 1,164,622 + MMJJ_RS06270 0.14 -4.2 1,164,622 + MMJJ_RS06270 0.14 -2.7 1,164,627 + MMJJ_RS06270 0.15 -3.4 1,164,731 + MMJJ_RS06270 0.33 -2.9 1,164,784 + MMJJ_RS06270 0.43 -0.6 1,164,788 + MMJJ_RS06270 0.43 -1.8 1,164,834 - MMJJ_RS06270 0.51 -0.9 1,164,850 - MMJJ_RS06270 0.54 -2.7 1,164,919 - MMJJ_RS06270 0.66 -2.1 1,164,919 - MMJJ_RS06270 0.66 -2.2 1,164,933 - MMJJ_RS06270 0.69 -1.9 1,164,945 + MMJJ_RS06270 0.71 -1.4 1,164,958 - MMJJ_RS06270 0.73 -1.8 1,164,959 + MMJJ_RS06270 0.73 -2.8 1,164,974 + MMJJ_RS06270 0.76 -3.1 1,165,005 - MMJJ_RS06270 0.81 -3.6 1,165,009 + MMJJ_RS06270 0.82 -1.3 1,165,094 - +1.5 1,165,094 - -0.2 1,165,095 + -2.0 1,165,095 + -1.2 1,165,155 + MMJJ_RS06275 0.10 +1.4 1,165,171 - MMJJ_RS06275 0.16 +1.4 1,165,175 - MMJJ_RS06275 0.17 +1.5 1,165,210 - MMJJ_RS06275 0.29 +0.8 1,165,363 - MMJJ_RS06275 0.80 +0.9 1,165,363 - MMJJ_RS06275 0.80 -0.4 1,165,364 + MMJJ_RS06275 0.81 +1.0 1,165,367 - MMJJ_RS06275 0.82 +0.9 1,165,367 - MMJJ_RS06275 0.82 -0.3 1,165,368 + MMJJ_RS06275 0.82 +1.6 1,165,374 + MMJJ_RS06275 0.84 -1.0 1,165,394 - +0.2 1,165,440 - -0.6 1,165,440 - +0.1 1,165,440 - +0.3 1,165,440 - -0.1 1,165,441 + +0.5 1,165,441 + +1.3 1,165,442 - +0.6 1,165,442 - +0.3 1,165,443 + +0.3 1,165,443 + -0.7 1,165,448 - +0.4 1,165,448 - -0.2 1,165,449 + +1.4 1,165,449 + +0.4 1,165,449 + -0.7 1,165,450 - -2.3 1,165,450 - -0.1 1,165,450 - +0.1 1,165,450 - -0.7 1,165,450 - -2.0 1,165,451 + -0.1 1,165,451 + -0.7 1,165,451 + -0.5 1,165,451 + -0.8 1,165,452 - +1.5 1,165,452 - -1.0 1,165,452 - +0.4 1,165,453 + -0.7 1,165,453 + -0.1 1,165,453 + -0.6 1,165,453 + +1.3 1,165,454 - +0.0 1,165,454 - -0.4 1,165,454 - +0.4 1,165,454 - +0.4 1,165,455 + -1.1 1,165,455 + -0.2 1,165,455 + +0.5 1,165,455 + -0.4 1,165,458 - +0.8 1,165,458 - +0.9 1,165,458 - +0.1 1,165,458 - +0.1 1,165,458 - +0.4
Or see this region's nucleotide sequence