Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06240 and MMJJ_RS06245 are separated by 68 nucleotides MMJJ_RS06245 and MMJJ_RS06250 are separated by 128 nucleotides MMJJ_RS06250 and MMJJ_RS06255 are separated by 130 nucleotides
MMJJ_RS06240: MMJJ_RS06240 - dihydropteroate synthase-like protein, at 1,157,773 to 1,159,344
_RS06240
MMJJ_RS06245: MMJJ_RS06245 - 30S ribosomal protein S15, at 1,159,413 to 1,159,868
_RS06245
MMJJ_RS06250: MMJJ_RS06250 - nicotinamide-nucleotide adenylyltransferase, at 1,159,997 to 1,160,512
_RS06250
MMJJ_RS06255: MMJJ_RS06255 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,160,643 to 1,162,265
_RS06255
Position (kb)
1159
1160
1161 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1159.321 kb on - strand at 1159.321 kb on - strand at 1159.322 kb on + strand at 1159.343 kb on + strand at 1159.361 kb on - strand at 1159.361 kb on - strand at 1159.362 kb on + strand at 1159.405 kb on - strand at 1159.486 kb on - strand, within MMJJ_RS06245 at 1159.857 kb on - strand at 1159.958 kb on - strand at 1159.958 kb on - strand at 1159.962 kb on - strand at 1159.976 kb on - strand at 1159.987 kb on - strand at 1159.988 kb on + strand at 1160.063 kb on - strand, within MMJJ_RS06250 at 1160.088 kb on + strand at 1160.152 kb on - strand, within MMJJ_RS06250 at 1160.182 kb on + strand, within MMJJ_RS06250 at 1160.198 kb on - strand, within MMJJ_RS06250 at 1160.236 kb on - strand at 1160.281 kb on - strand at 1160.281 kb on - strand at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.283 kb on - strand, within MMJJ_RS06250 at 1160.288 kb on - strand, within MMJJ_RS06250 at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.545 kb on - strand at 1160.546 kb on + strand at 1160.551 kb on - strand at 1160.552 kb on + strand at 1160.556 kb on - strand at 1160.557 kb on + strand at 1160.580 kb on + strand at 1160.583 kb on + strand at 1160.586 kb on - strand at 1160.586 kb on - strand at 1160.587 kb on + strand at 1160.593 kb on + strand at 1160.593 kb on + strand at 1160.594 kb on - strand at 1160.595 kb on + strand at 1160.605 kb on - strand at 1160.606 kb on + strand at 1160.607 kb on - strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.608 kb on + strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.609 kb on - strand at 1160.610 kb on + strand at 1160.610 kb on + strand at 1160.613 kb on + strand at 1160.620 kb on + strand at 1160.666 kb on + strand at 1160.689 kb on - strand at 1160.690 kb on + strand at 1160.973 kb on + strand, within MMJJ_RS06255 at 1160.998 kb on + strand, within MMJJ_RS06255 at 1161.036 kb on - strand, within MMJJ_RS06255 at 1161.037 kb on + strand, within MMJJ_RS06255 at 1161.052 kb on + strand, within MMJJ_RS06255 at 1161.112 kb on - strand, within MMJJ_RS06255 at 1161.366 kb on + strand at 1161.422 kb on - strand at 1161.465 kb on - strand, within MMJJ_RS06255 at 1161.466 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,159,321 - -0.7 1,159,321 - -0.8 1,159,322 + +1.2 1,159,343 + +1.8 1,159,361 - +1.7 1,159,361 - -0.4 1,159,362 + -0.5 1,159,405 - +1.4 1,159,486 - MMJJ_RS06245 0.16 -1.4 1,159,857 - -0.2 1,159,958 - +0.1 1,159,958 - -1.6 1,159,962 - -0.3 1,159,976 - +0.3 1,159,987 - +1.2 1,159,988 + +1.0 1,160,063 - MMJJ_RS06250 0.13 -0.0 1,160,088 + +0.4 1,160,152 - MMJJ_RS06250 0.30 -1.4 1,160,182 + MMJJ_RS06250 0.36 -2.1 1,160,198 - MMJJ_RS06250 0.39 -0.4 1,160,236 - +1.4 1,160,281 - -0.9 1,160,281 - -0.2 1,160,283 - MMJJ_RS06250 0.55 -1.9 1,160,283 - MMJJ_RS06250 0.55 -1.4 1,160,288 - MMJJ_RS06250 0.56 -0.7 1,160,545 - -2.0 1,160,545 - -0.9 1,160,545 - +0.2 1,160,545 - -4.4 1,160,545 - -3.4 1,160,546 + -0.9 1,160,551 - -1.1 1,160,552 + -2.2 1,160,556 - -1.3 1,160,557 + -0.9 1,160,580 + -0.6 1,160,583 + +0.4 1,160,586 - -3.1 1,160,586 - -0.0 1,160,587 + -0.4 1,160,593 + -0.6 1,160,593 + +0.1 1,160,594 - +0.3 1,160,595 + -0.6 1,160,605 - +1.1 1,160,606 + +0.3 1,160,607 - -1.9 1,160,608 + +0.7 1,160,608 + +0.2 1,160,608 + -2.3 1,160,608 + -0.2 1,160,608 + -0.2 1,160,609 - -1.8 1,160,609 - -1.4 1,160,609 - -0.1 1,160,610 + -1.6 1,160,610 + -0.6 1,160,613 + -0.9 1,160,620 + -3.3 1,160,666 + +2.0 1,160,689 - -0.7 1,160,690 + -1.4 1,160,973 + MMJJ_RS06255 0.20 -0.0 1,160,998 + MMJJ_RS06255 0.22 -1.0 1,161,036 - MMJJ_RS06255 0.24 -3.0 1,161,037 + MMJJ_RS06255 0.24 -2.5 1,161,052 + MMJJ_RS06255 0.25 -3.6 1,161,112 - MMJJ_RS06255 0.29 -1.6 1,161,366 + -0.9 1,161,422 - -0.2 1,161,465 - MMJJ_RS06255 0.51 -3.1 1,161,466 + +0.4
Or see this region's nucleotide sequence