Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS06100 and MMJJ_RS06105 are separated by 123 nucleotides MMJJ_RS06105 and MMJJ_RS06110 are separated by 9 nucleotides MMJJ_RS06110 and MMJJ_RS06115 are separated by 30 nucleotides
MMJJ_RS06100: MMJJ_RS06100 - rubrerythrin, at 1,132,646 to 1,132,954
_RS06100
MMJJ_RS06105: MMJJ_RS06105 - DUF99 family protein, at 1,133,078 to 1,133,629
_RS06105
MMJJ_RS06110: MMJJ_RS06110 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 1,133,639 to 1,134,829
_RS06110
MMJJ_RS06115: MMJJ_RS06115 - pyruvate kinase, at 1,134,860 to 1,136,203
_RS06115
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1132.701 kb on - strand, within MMJJ_RS06100 at 1132.702 kb on + strand, within MMJJ_RS06100 at 1132.743 kb on + strand, within MMJJ_RS06100 at 1132.761 kb on + strand, within MMJJ_RS06100 at 1132.761 kb on + strand, within MMJJ_RS06100 at 1132.778 kb on - strand, within MMJJ_RS06100 at 1132.778 kb on - strand, within MMJJ_RS06100 at 1132.778 kb on - strand, within MMJJ_RS06100 at 1132.779 kb on + strand, within MMJJ_RS06100 at 1132.779 kb on + strand, within MMJJ_RS06100 at 1132.779 kb on + strand, within MMJJ_RS06100 at 1132.780 kb on - strand, within MMJJ_RS06100 at 1132.781 kb on + strand, within MMJJ_RS06100 at 1132.781 kb on + strand, within MMJJ_RS06100 at 1132.788 kb on + strand, within MMJJ_RS06100 at 1132.834 kb on - strand, within MMJJ_RS06100 at 1132.834 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.836 kb on - strand, within MMJJ_RS06100 at 1132.837 kb on + strand, within MMJJ_RS06100 at 1132.837 kb on + strand, within MMJJ_RS06100 at 1132.878 kb on + strand, within MMJJ_RS06100 at 1132.879 kb on - strand, within MMJJ_RS06100 at 1132.879 kb on - strand, within MMJJ_RS06100 at 1132.879 kb on - strand, within MMJJ_RS06100 at 1132.890 kb on + strand, within MMJJ_RS06100 at 1132.980 kb on + strand at 1133.001 kb on - strand at 1133.011 kb on - strand at 1133.023 kb on - strand at 1133.024 kb on + strand at 1133.027 kb on - strand at 1133.027 kb on - strand at 1133.027 kb on - strand at 1133.027 kb on - strand at 1133.028 kb on + strand at 1133.028 kb on + strand at 1133.028 kb on + strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.029 kb on - strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.030 kb on + strand at 1133.031 kb on - strand at 1133.031 kb on - strand at 1133.031 kb on - strand at 1133.032 kb on + strand at 1133.032 kb on + strand at 1133.040 kb on - strand at 1133.040 kb on - strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.041 kb on + strand at 1133.066 kb on - strand at 1133.067 kb on + strand at 1133.067 kb on + strand at 1133.067 kb on + strand at 1133.079 kb on - strand at 1133.111 kb on + strand at 1133.147 kb on + strand, within MMJJ_RS06105 at 1133.147 kb on + strand, within MMJJ_RS06105 at 1133.147 kb on + strand, within MMJJ_RS06105 at 1133.165 kb on - strand, within MMJJ_RS06105 at 1133.165 kb on - strand, within MMJJ_RS06105 at 1133.165 kb on - strand, within MMJJ_RS06105 at 1133.166 kb on + strand, within MMJJ_RS06105 at 1133.166 kb on + strand, within MMJJ_RS06105 at 1133.210 kb on - strand, within MMJJ_RS06105 at 1133.210 kb on - strand, within MMJJ_RS06105 at 1133.212 kb on - strand, within MMJJ_RS06105 at 1133.212 kb on - strand, within MMJJ_RS06105 at 1133.212 kb on - strand, within MMJJ_RS06105 at 1133.213 kb on + strand, within MMJJ_RS06105 at 1133.307 kb on + strand, within MMJJ_RS06105 at 1133.368 kb on - strand, within MMJJ_RS06105 at 1133.368 kb on - strand, within MMJJ_RS06105 at 1133.384 kb on + strand, within MMJJ_RS06105 at 1133.386 kb on - strand, within MMJJ_RS06105 at 1133.387 kb on + strand, within MMJJ_RS06105 at 1133.426 kb on - strand, within MMJJ_RS06105 at 1133.434 kb on - strand, within MMJJ_RS06105 at 1133.443 kb on - strand, within MMJJ_RS06105 at 1133.504 kb on + strand, within MMJJ_RS06105 at 1133.510 kb on - strand, within MMJJ_RS06105 at 1133.510 kb on - strand, within MMJJ_RS06105 at 1133.511 kb on + strand, within MMJJ_RS06105 at 1133.541 kb on - strand, within MMJJ_RS06105 at 1133.541 kb on - strand, within MMJJ_RS06105 at 1133.541 kb on - strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.542 kb on + strand, within MMJJ_RS06105 at 1133.567 kb on + strand, within MMJJ_RS06105 at 1133.596 kb on - strand at 1133.597 kb on + strand at 1133.602 kb on - strand at 1133.620 kb on - strand at 1133.620 kb on - strand at 1133.620 kb on - strand at 1133.620 kb on - strand at 1133.621 kb on + strand at 1133.862 kb on - strand, within MMJJ_RS06110 at 1134.021 kb on + strand, within MMJJ_RS06110 at 1134.096 kb on - strand, within MMJJ_RS06110 at 1134.099 kb on - strand, within MMJJ_RS06110 at 1134.203 kb on + strand, within MMJJ_RS06110 at 1134.530 kb on + strand, within MMJJ_RS06110 at 1134.863 kb on - strand at 1134.863 kb on - strand at 1134.927 kb on - strand at 1134.928 kb on + strand at 1134.928 kb on + strand at 1134.944 kb on - strand at 1134.945 kb on + strand at 1134.964 kb on - strand at 1135.053 kb on + strand, within MMJJ_RS06115 at 1135.075 kb on - strand, within MMJJ_RS06115 at 1135.076 kb on + strand, within MMJJ_RS06115 at 1135.087 kb on - strand, within MMJJ_RS06115 at 1135.200 kb on - strand, within MMJJ_RS06115 at 1135.200 kb on - strand, within MMJJ_RS06115 at 1135.271 kb on + strand, within MMJJ_RS06115 at 1135.271 kb on + strand, within MMJJ_RS06115 at 1135.439 kb on - strand, within MMJJ_RS06115 at 1135.488 kb on + strand, within MMJJ_RS06115 at 1135.498 kb on + strand, within MMJJ_RS06115 at 1135.541 kb on - strand, within MMJJ_RS06115 at 1135.542 kb on + strand, within MMJJ_RS06115 at 1135.649 kb on - strand, within MMJJ_RS06115 at 1135.695 kb on - strand, within MMJJ_RS06115 at 1135.734 kb on + strand, within MMJJ_RS06115 at 1135.814 kb on - strand, within MMJJ_RS06115 at 1135.814 kb on - strand, within MMJJ_RS06115 at 1135.829 kb on - strand, within MMJJ_RS06115
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,132,701 - MMJJ_RS06100 0.18 -1.9 1,132,702 + MMJJ_RS06100 0.18 +0.2 1,132,743 + MMJJ_RS06100 0.31 +2.1 1,132,761 + MMJJ_RS06100 0.37 -0.1 1,132,761 + MMJJ_RS06100 0.37 +0.8 1,132,778 - MMJJ_RS06100 0.43 +0.7 1,132,778 - MMJJ_RS06100 0.43 -0.1 1,132,778 - MMJJ_RS06100 0.43 +1.4 1,132,779 + MMJJ_RS06100 0.43 +1.6 1,132,779 + MMJJ_RS06100 0.43 +1.3 1,132,779 + MMJJ_RS06100 0.43 +0.7 1,132,780 - MMJJ_RS06100 0.43 -0.4 1,132,781 + MMJJ_RS06100 0.44 +1.0 1,132,781 + MMJJ_RS06100 0.44 +0.7 1,132,788 + MMJJ_RS06100 0.46 -2.3 1,132,834 - MMJJ_RS06100 0.61 -0.1 1,132,834 - MMJJ_RS06100 0.61 -0.4 1,132,836 - MMJJ_RS06100 0.61 +0.8 1,132,836 - MMJJ_RS06100 0.61 +2.0 1,132,836 - MMJJ_RS06100 0.61 +0.3 1,132,836 - MMJJ_RS06100 0.61 +0.5 1,132,836 - MMJJ_RS06100 0.61 +2.3 1,132,836 - MMJJ_RS06100 0.61 -1.2 1,132,837 + MMJJ_RS06100 0.62 -0.5 1,132,837 + MMJJ_RS06100 0.62 +0.5 1,132,878 + MMJJ_RS06100 0.75 +1.1 1,132,879 - MMJJ_RS06100 0.75 -0.3 1,132,879 - MMJJ_RS06100 0.75 +1.1 1,132,879 - MMJJ_RS06100 0.75 -1.2 1,132,890 + MMJJ_RS06100 0.79 -0.1 1,132,980 + +0.5 1,133,001 - +1.9 1,133,011 - +1.1 1,133,023 - -0.7 1,133,024 + +0.1 1,133,027 - -0.4 1,133,027 - +0.9 1,133,027 - +0.5 1,133,027 - -0.4 1,133,028 + +1.5 1,133,028 + +1.2 1,133,028 + -0.1 1,133,029 - -0.6 1,133,029 - +0.7 1,133,029 - +0.7 1,133,029 - +0.2 1,133,029 - +1.4 1,133,029 - +1.3 1,133,029 - +1.0 1,133,030 + -0.3 1,133,030 + -0.4 1,133,030 + -1.6 1,133,030 + +0.8 1,133,030 + +1.1 1,133,030 + -0.1 1,133,030 + +0.9 1,133,031 - +1.8 1,133,031 - +1.0 1,133,031 - +0.8 1,133,032 + -1.2 1,133,032 + +0.7 1,133,040 - +0.6 1,133,040 - -0.4 1,133,041 + +0.8 1,133,041 + +0.8 1,133,041 + +0.9 1,133,041 + +0.2 1,133,041 + -0.2 1,133,041 + -0.2 1,133,041 + +1.1 1,133,066 - +0.2 1,133,067 + +0.4 1,133,067 + -0.2 1,133,067 + -1.6 1,133,079 - +0.1 1,133,111 + -1.0 1,133,147 + MMJJ_RS06105 0.12 +0.8 1,133,147 + MMJJ_RS06105 0.12 +1.0 1,133,147 + MMJJ_RS06105 0.12 +1.1 1,133,165 - MMJJ_RS06105 0.16 +1.0 1,133,165 - MMJJ_RS06105 0.16 -0.5 1,133,165 - MMJJ_RS06105 0.16 +0.1 1,133,166 + MMJJ_RS06105 0.16 +0.0 1,133,166 + MMJJ_RS06105 0.16 +1.8 1,133,210 - MMJJ_RS06105 0.24 +1.0 1,133,210 - MMJJ_RS06105 0.24 -2.5 1,133,212 - MMJJ_RS06105 0.24 +1.3 1,133,212 - MMJJ_RS06105 0.24 +0.6 1,133,212 - MMJJ_RS06105 0.24 +1.5 1,133,213 + MMJJ_RS06105 0.24 +0.1 1,133,307 + MMJJ_RS06105 0.41 +1.6 1,133,368 - MMJJ_RS06105 0.53 +0.5 1,133,368 - MMJJ_RS06105 0.53 +1.2 1,133,384 + MMJJ_RS06105 0.55 +1.2 1,133,386 - MMJJ_RS06105 0.56 +0.5 1,133,387 + MMJJ_RS06105 0.56 +0.7 1,133,426 - MMJJ_RS06105 0.63 +0.5 1,133,434 - MMJJ_RS06105 0.64 +0.0 1,133,443 - MMJJ_RS06105 0.66 +1.3 1,133,504 + MMJJ_RS06105 0.77 +0.2 1,133,510 - MMJJ_RS06105 0.78 +0.9 1,133,510 - MMJJ_RS06105 0.78 +0.4 1,133,511 + MMJJ_RS06105 0.78 -1.8 1,133,541 - MMJJ_RS06105 0.84 -1.4 1,133,541 - MMJJ_RS06105 0.84 -1.2 1,133,541 - MMJJ_RS06105 0.84 +1.3 1,133,542 + MMJJ_RS06105 0.84 -1.2 1,133,542 + MMJJ_RS06105 0.84 +0.0 1,133,542 + MMJJ_RS06105 0.84 +1.4 1,133,542 + MMJJ_RS06105 0.84 +1.3 1,133,542 + MMJJ_RS06105 0.84 +1.4 1,133,542 + MMJJ_RS06105 0.84 +1.0 1,133,542 + MMJJ_RS06105 0.84 +1.2 1,133,567 + MMJJ_RS06105 0.89 +0.2 1,133,596 - +1.4 1,133,597 + +0.9 1,133,602 - -0.4 1,133,620 - +0.7 1,133,620 - +1.4 1,133,620 - -0.2 1,133,620 - -1.7 1,133,621 + +0.7 1,133,862 - MMJJ_RS06110 0.19 -1.9 1,134,021 + MMJJ_RS06110 0.32 -0.7 1,134,096 - MMJJ_RS06110 0.38 -1.7 1,134,099 - MMJJ_RS06110 0.39 -1.0 1,134,203 + MMJJ_RS06110 0.47 -1.5 1,134,530 + MMJJ_RS06110 0.75 +0.3 1,134,863 - -1.3 1,134,863 - -0.7 1,134,927 - -0.3 1,134,928 + -0.1 1,134,928 + -0.7 1,134,944 - +1.0 1,134,945 + +1.3 1,134,964 - -0.0 1,135,053 + MMJJ_RS06115 0.14 +0.9 1,135,075 - MMJJ_RS06115 0.16 +0.3 1,135,076 + MMJJ_RS06115 0.16 -0.3 1,135,087 - MMJJ_RS06115 0.17 +1.0 1,135,200 - MMJJ_RS06115 0.25 -0.8 1,135,200 - MMJJ_RS06115 0.25 +0.3 1,135,271 + MMJJ_RS06115 0.31 -0.4 1,135,271 + MMJJ_RS06115 0.31 +1.1 1,135,439 - MMJJ_RS06115 0.43 +0.0 1,135,488 + MMJJ_RS06115 0.47 -1.7 1,135,498 + MMJJ_RS06115 0.47 -0.3 1,135,541 - MMJJ_RS06115 0.51 -0.1 1,135,542 + MMJJ_RS06115 0.51 +0.5 1,135,649 - MMJJ_RS06115 0.59 +0.5 1,135,695 - MMJJ_RS06115 0.62 -0.3 1,135,734 + MMJJ_RS06115 0.65 -0.2 1,135,814 - MMJJ_RS06115 0.71 +0.3 1,135,814 - MMJJ_RS06115 0.71 -0.3 1,135,829 - MMJJ_RS06115 0.72 +1.1
Or see this region's nucleotide sequence