Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS05405 and MMJJ_RS05410 are separated by 42 nucleotides MMJJ_RS05410 and MMJJ_RS05415 are separated by 27 nucleotides MMJJ_RS05415 and MMJJ_RS05420 are separated by 10 nucleotides
MMJJ_RS05405: MMJJ_RS05405 - DNA cytosine methyltransferase, at 1,003,602 to 1,004,669
_RS05405
MMJJ_RS05410: MMJJ_RS05410 - RecB-family nuclease, at 1,004,712 to 1,005,137
_RS05410
MMJJ_RS05415: MMJJ_RS05415 - hypothetical protein, at 1,005,165 to 1,006,298
_RS05415
MMJJ_RS05420: MMJJ_RS05420 - DNA-directed DNA polymerase II small subunit, at 1,006,309 to 1,008,066
_RS05420
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1004.181 kb on - strand, within MMJJ_RS05405 at 1004.240 kb on - strand, within MMJJ_RS05405 at 1004.276 kb on - strand, within MMJJ_RS05405 at 1004.288 kb on + strand, within MMJJ_RS05405 at 1004.312 kb on - strand, within MMJJ_RS05405 at 1004.399 kb on - strand, within MMJJ_RS05405 at 1004.425 kb on - strand, within MMJJ_RS05405 at 1004.426 kb on + strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.433 kb on - strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.434 kb on + strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.489 kb on - strand, within MMJJ_RS05405 at 1004.516 kb on + strand, within MMJJ_RS05405 at 1004.579 kb on - strand at 1004.579 kb on - strand at 1004.579 kb on - strand at 1004.580 kb on + strand at 1004.589 kb on - strand at 1004.590 kb on + strand at 1004.590 kb on + strand at 1004.655 kb on - strand at 1004.656 kb on + strand at 1004.661 kb on + strand at 1004.663 kb on - strand at 1004.663 kb on - strand at 1004.667 kb on - strand at 1004.668 kb on + strand at 1004.690 kb on + strand at 1004.697 kb on + strand at 1004.800 kb on - strand, within MMJJ_RS05410 at 1004.801 kb on + strand, within MMJJ_RS05410 at 1004.804 kb on - strand, within MMJJ_RS05410 at 1004.805 kb on + strand, within MMJJ_RS05410 at 1004.863 kb on - strand, within MMJJ_RS05410 at 1004.863 kb on - strand, within MMJJ_RS05410 at 1004.864 kb on + strand, within MMJJ_RS05410 at 1004.935 kb on - strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.936 kb on + strand, within MMJJ_RS05410 at 1004.939 kb on + strand, within MMJJ_RS05410 at 1004.941 kb on - strand, within MMJJ_RS05410 at 1004.981 kb on - strand, within MMJJ_RS05410 at 1004.988 kb on + strand, within MMJJ_RS05410 at 1004.988 kb on + strand, within MMJJ_RS05410 at 1005.071 kb on - strand, within MMJJ_RS05410 at 1005.072 kb on + strand, within MMJJ_RS05410 at 1005.072 kb on + strand, within MMJJ_RS05410 at 1005.076 kb on - strand, within MMJJ_RS05410 at 1005.084 kb on + strand, within MMJJ_RS05410 at 1005.136 kb on + strand at 1005.140 kb on - strand at 1005.140 kb on - strand at 1005.141 kb on + strand at 1005.143 kb on + strand at 1005.381 kb on - strand, within MMJJ_RS05415 at 1005.435 kb on + strand at 1005.584 kb on + strand, within MMJJ_RS05415 at 1006.051 kb on - strand, within MMJJ_RS05415 at 1006.095 kb on + strand, within MMJJ_RS05415 at 1006.142 kb on + strand, within MMJJ_RS05415 at 1006.258 kb on - strand at 1006.537 kb on - strand, within MMJJ_RS05420 at 1006.537 kb on - strand, within MMJJ_RS05420 at 1006.875 kb on + strand at 1007.061 kb on + strand, within MMJJ_RS05420
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 1,004,181 - MMJJ_RS05405 0.54 -1.6 1,004,240 - MMJJ_RS05405 0.60 -0.9 1,004,276 - MMJJ_RS05405 0.63 -0.2 1,004,288 + MMJJ_RS05405 0.64 +1.5 1,004,312 - MMJJ_RS05405 0.66 +1.1 1,004,399 - MMJJ_RS05405 0.75 -0.4 1,004,425 - MMJJ_RS05405 0.77 +0.4 1,004,426 + MMJJ_RS05405 0.77 -1.1 1,004,433 - MMJJ_RS05405 0.78 +2.1 1,004,433 - MMJJ_RS05405 0.78 +1.8 1,004,433 - MMJJ_RS05405 0.78 +0.6 1,004,433 - MMJJ_RS05405 0.78 +0.9 1,004,434 + MMJJ_RS05405 0.78 +1.5 1,004,434 + MMJJ_RS05405 0.78 -0.2 1,004,489 - MMJJ_RS05405 0.83 +0.8 1,004,489 - MMJJ_RS05405 0.83 -0.3 1,004,489 - MMJJ_RS05405 0.83 +1.1 1,004,489 - MMJJ_RS05405 0.83 -0.0 1,004,516 + MMJJ_RS05405 0.86 -0.0 1,004,579 - -1.2 1,004,579 - +1.1 1,004,579 - +1.1 1,004,580 + +0.1 1,004,589 - +1.0 1,004,590 + +1.3 1,004,590 + +0.5 1,004,655 - +1.1 1,004,656 + +1.9 1,004,661 + +0.4 1,004,663 - -1.3 1,004,663 - -1.5 1,004,667 - -1.9 1,004,668 + +1.1 1,004,690 + -4.0 1,004,697 + -0.6 1,004,800 - MMJJ_RS05410 0.21 +1.6 1,004,801 + MMJJ_RS05410 0.21 -0.2 1,004,804 - MMJJ_RS05410 0.22 +1.1 1,004,805 + MMJJ_RS05410 0.22 -0.7 1,004,863 - MMJJ_RS05410 0.35 +1.6 1,004,863 - MMJJ_RS05410 0.35 +0.1 1,004,864 + MMJJ_RS05410 0.36 +0.1 1,004,935 - MMJJ_RS05410 0.52 -0.1 1,004,936 + MMJJ_RS05410 0.53 -1.2 1,004,936 + MMJJ_RS05410 0.53 -0.3 1,004,936 + MMJJ_RS05410 0.53 +0.6 1,004,939 + MMJJ_RS05410 0.53 -0.4 1,004,941 - MMJJ_RS05410 0.54 -0.7 1,004,981 - MMJJ_RS05410 0.63 +0.9 1,004,988 + MMJJ_RS05410 0.65 -0.3 1,004,988 + MMJJ_RS05410 0.65 +0.6 1,005,071 - MMJJ_RS05410 0.84 +2.9 1,005,072 + MMJJ_RS05410 0.85 -0.7 1,005,072 + MMJJ_RS05410 0.85 +1.0 1,005,076 - MMJJ_RS05410 0.85 +0.6 1,005,084 + MMJJ_RS05410 0.87 +1.4 1,005,136 + +1.2 1,005,140 - +0.8 1,005,140 - -0.4 1,005,141 + +1.4 1,005,143 + +0.7 1,005,381 - MMJJ_RS05415 0.19 -1.9 1,005,435 + +3.9 1,005,584 + MMJJ_RS05415 0.37 -0.4 1,006,051 - MMJJ_RS05415 0.78 -1.2 1,006,095 + MMJJ_RS05415 0.82 -1.4 1,006,142 + MMJJ_RS05415 0.86 +0.1 1,006,258 - -0.4 1,006,537 - MMJJ_RS05420 0.13 -1.2 1,006,537 - MMJJ_RS05420 0.13 -2.9 1,006,875 + +0.1 1,007,061 + MMJJ_RS05420 0.43 -0.2
Or see this region's nucleotide sequence