Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04755 and MMJJ_RS04760 are separated by 28 nucleotides MMJJ_RS04760 and MMJJ_RS04765 are separated by 40 nucleotides MMJJ_RS04765 and MMJJ_RS04770 are separated by 18 nucleotides
MMJJ_RS04755: MMJJ_RS04755 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB, at 892,054 to 893,079
_RS04755
MMJJ_RS04760: MMJJ_RS04760 - 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC, at 893,108 to 894,640
_RS04760
MMJJ_RS04765: MMJJ_RS04765 - metal-dependent hydrolase, at 894,681 to 895,355
_RS04765
MMJJ_RS04770: MMJJ_RS04770 - formate-dependent phosphoribosylglycinamide formyltransferase, at 895,374 to 896,540
_RS04770
Position (kb) 893
894
895 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 892.112 kb on - strand at 892.112 kb on - strand at 892.125 kb on + strand at 892.152 kb on + strand at 892.155 kb on - strand at 892.156 kb on + strand at 892.181 kb on - strand, within MMJJ_RS04755 at 892.182 kb on + strand, within MMJJ_RS04755 at 892.210 kb on + strand at 892.210 kb on + strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.256 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.260 kb on - strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.261 kb on + strand, within MMJJ_RS04755 at 892.264 kb on + strand, within MMJJ_RS04755 at 892.291 kb on - strand, within MMJJ_RS04755 at 892.292 kb on + strand, within MMJJ_RS04755 at 892.328 kb on + strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.331 kb on - strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.332 kb on + strand, within MMJJ_RS04755 at 892.445 kb on - strand, within MMJJ_RS04755 at 892.457 kb on - strand, within MMJJ_RS04755 at 892.484 kb on - strand, within MMJJ_RS04755 at 892.514 kb on - strand, within MMJJ_RS04755 at 892.546 kb on - strand, within MMJJ_RS04755 at 892.548 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand, within MMJJ_RS04755 at 892.553 kb on - strand at 892.557 kb on - strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.558 kb on + strand, within MMJJ_RS04755 at 892.563 kb on + strand, within MMJJ_RS04755 at 892.637 kb on - strand, within MMJJ_RS04755 at 892.671 kb on + strand, within MMJJ_RS04755 at 892.676 kb on - strand, within MMJJ_RS04755 at 892.694 kb on - strand, within MMJJ_RS04755 at 892.699 kb on + strand, within MMJJ_RS04755 at 892.719 kb on - strand, within MMJJ_RS04755 at 892.720 kb on + strand, within MMJJ_RS04755 at 892.773 kb on + strand, within MMJJ_RS04755 at 892.790 kb on + strand, within MMJJ_RS04755 at 892.860 kb on + strand, within MMJJ_RS04755 at 892.888 kb on + strand, within MMJJ_RS04755 at 892.964 kb on - strand, within MMJJ_RS04755 at 892.992 kb on - strand at 893.036 kb on - strand at 893.037 kb on + strand at 893.037 kb on + strand at 893.045 kb on - strand at 893.073 kb on + strand at 893.077 kb on - strand at 893.077 kb on - strand at 893.078 kb on + strand at 893.087 kb on + strand at 893.096 kb on - strand at 893.142 kb on - strand at 893.401 kb on + strand, within MMJJ_RS04760 at 893.401 kb on + strand, within MMJJ_RS04760 at 893.478 kb on + strand, within MMJJ_RS04760 at 893.479 kb on - strand, within MMJJ_RS04760 at 893.482 kb on - strand, within MMJJ_RS04760 at 893.653 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.811 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.874 kb on - strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.875 kb on + strand, within MMJJ_RS04760 at 893.880 kb on - strand, within MMJJ_RS04760 at 893.881 kb on + strand, within MMJJ_RS04760 at 893.899 kb on - strand, within MMJJ_RS04760 at 894.037 kb on + strand, within MMJJ_RS04760 at 894.037 kb on + strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.073 kb on - strand, within MMJJ_RS04760 at 894.074 kb on + strand, within MMJJ_RS04760 at 894.229 kb on - strand, within MMJJ_RS04760 at 894.246 kb on - strand, within MMJJ_RS04760 at 894.246 kb on - strand, within MMJJ_RS04760 at 894.246 kb on - strand, within MMJJ_RS04760 at 894.246 kb on - strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.247 kb on + strand, within MMJJ_RS04760 at 894.248 kb on - strand, within MMJJ_RS04760 at 894.269 kb on - strand, within MMJJ_RS04760 at 894.270 kb on + strand, within MMJJ_RS04760 at 894.276 kb on - strand, within MMJJ_RS04760 at 894.276 kb on - strand, within MMJJ_RS04760 at 894.302 kb on + strand, within MMJJ_RS04760 at 894.305 kb on + strand, within MMJJ_RS04760 at 894.317 kb on - strand, within MMJJ_RS04760 at 894.318 kb on + strand, within MMJJ_RS04760 at 894.318 kb on + strand, within MMJJ_RS04760 at 894.329 kb on - strand, within MMJJ_RS04760 at 894.329 kb on - strand, within MMJJ_RS04760 at 894.366 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.401 kb on - strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.402 kb on + strand, within MMJJ_RS04760 at 894.404 kb on + strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.444 kb on - strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.445 kb on + strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.446 kb on - strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.447 kb on + strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.450 kb on - strand, within MMJJ_RS04760 at 894.496 kb on + strand at 894.511 kb on + strand at 894.518 kb on - strand at 894.519 kb on + strand at 894.519 kb on + strand at 894.575 kb on + strand at 894.577 kb on - strand at 894.578 kb on + strand at 894.578 kb on + strand at 894.601 kb on + strand at 894.634 kb on + strand at 894.634 kb on + strand at 894.634 kb on + strand at 894.698 kb on - strand at 894.699 kb on + strand at 894.699 kb on + strand at 894.732 kb on + strand at 894.732 kb on + strand at 894.761 kb on + strand, within MMJJ_RS04765 at 894.788 kb on + strand, within MMJJ_RS04765 at 894.799 kb on - strand, within MMJJ_RS04765 at 894.812 kb on + strand, within MMJJ_RS04765 at 894.812 kb on + strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.828 kb on - strand, within MMJJ_RS04765 at 894.829 kb on + strand, within MMJJ_RS04765 at 894.829 kb on + strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.830 kb on - strand, within MMJJ_RS04765 at 894.831 kb on + strand, within MMJJ_RS04765 at 894.831 kb on + strand, within MMJJ_RS04765 at 894.846 kb on + strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.867 kb on - strand, within MMJJ_RS04765 at 894.871 kb on - strand, within MMJJ_RS04765 at 894.871 kb on - strand, within MMJJ_RS04765 at 894.885 kb on - strand, within MMJJ_RS04765 at 894.935 kb on - strand, within MMJJ_RS04765 at 894.949 kb on + strand, within MMJJ_RS04765 at 894.995 kb on - strand, within MMJJ_RS04765 at 895.088 kb on - strand, within MMJJ_RS04765 at 895.089 kb on + strand, within MMJJ_RS04765 at 895.160 kb on - strand, within MMJJ_RS04765 at 895.161 kb on + strand, within MMJJ_RS04765 at 895.185 kb on - strand, within MMJJ_RS04765 at 895.190 kb on + strand, within MMJJ_RS04765 at 895.209 kb on - strand, within MMJJ_RS04765 at 895.277 kb on - strand, within MMJJ_RS04765 at 895.287 kb on + strand, within MMJJ_RS04765 at 895.599 kb on - strand, within MMJJ_RS04770
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 892,112 - -1.9 892,112 - -1.0 892,125 + -1.8 892,152 + -2.1 892,155 - -1.4 892,156 + -1.5 892,181 - MMJJ_RS04755 0.12 -3.0 892,182 + MMJJ_RS04755 0.12 -1.2 892,210 + +0.0 892,210 + MMJJ_RS04755 0.15 -1.5 892,256 - MMJJ_RS04755 0.20 -1.1 892,256 - MMJJ_RS04755 0.20 +0.1 892,260 - MMJJ_RS04755 0.20 -1.9 892,260 - MMJJ_RS04755 0.20 -2.5 892,260 - MMJJ_RS04755 0.20 -1.6 892,260 - MMJJ_RS04755 0.20 -2.0 892,261 + MMJJ_RS04755 0.20 -0.9 892,261 + MMJJ_RS04755 0.20 -0.8 892,261 + MMJJ_RS04755 0.20 -3.7 892,264 + MMJJ_RS04755 0.20 -1.2 892,291 - MMJJ_RS04755 0.23 -3.0 892,292 + MMJJ_RS04755 0.23 -3.3 892,328 + MMJJ_RS04755 0.27 -1.2 892,331 - MMJJ_RS04755 0.27 -3.3 892,331 - MMJJ_RS04755 0.27 -2.6 892,332 + MMJJ_RS04755 0.27 +0.7 892,332 + MMJJ_RS04755 0.27 -3.3 892,445 - MMJJ_RS04755 0.38 -1.5 892,457 - MMJJ_RS04755 0.39 -1.5 892,484 - MMJJ_RS04755 0.42 -1.3 892,514 - MMJJ_RS04755 0.45 -3.6 892,546 - MMJJ_RS04755 0.48 -0.8 892,548 - MMJJ_RS04755 0.48 -0.7 892,553 - MMJJ_RS04755 0.49 -3.6 892,553 - MMJJ_RS04755 0.49 -2.1 892,553 - -1.8 892,557 - MMJJ_RS04755 0.49 -3.2 892,558 + MMJJ_RS04755 0.49 -0.2 892,558 + MMJJ_RS04755 0.49 -1.8 892,563 + MMJJ_RS04755 0.50 -2.0 892,637 - MMJJ_RS04755 0.57 -1.0 892,671 + MMJJ_RS04755 0.60 -1.4 892,676 - MMJJ_RS04755 0.61 -2.7 892,694 - MMJJ_RS04755 0.62 -1.9 892,699 + MMJJ_RS04755 0.63 -0.7 892,719 - MMJJ_RS04755 0.65 -2.7 892,720 + MMJJ_RS04755 0.65 -1.8 892,773 + MMJJ_RS04755 0.70 -1.8 892,790 + MMJJ_RS04755 0.72 -2.1 892,860 + MMJJ_RS04755 0.79 -1.3 892,888 + MMJJ_RS04755 0.81 +0.5 892,964 - MMJJ_RS04755 0.89 -2.7 892,992 - -0.7 893,036 - +0.4 893,037 + -1.7 893,037 + -2.7 893,045 - -1.2 893,073 + -0.4 893,077 - -1.1 893,077 - +0.7 893,078 + -0.2 893,087 + -2.0 893,096 - -1.4 893,142 - -2.6 893,401 + MMJJ_RS04760 0.19 -1.7 893,401 + MMJJ_RS04760 0.19 -1.2 893,478 + MMJJ_RS04760 0.24 -3.2 893,479 - MMJJ_RS04760 0.24 -2.5 893,482 - MMJJ_RS04760 0.24 -1.9 893,653 - MMJJ_RS04760 0.36 -2.0 893,811 - MMJJ_RS04760 0.46 -1.4 893,811 - MMJJ_RS04760 0.46 -3.1 893,811 - MMJJ_RS04760 0.46 -4.5 893,874 - MMJJ_RS04760 0.50 -0.6 893,874 - MMJJ_RS04760 0.50 -3.0 893,874 - MMJJ_RS04760 0.50 -1.1 893,875 + MMJJ_RS04760 0.50 -2.5 893,875 + MMJJ_RS04760 0.50 -1.7 893,875 + MMJJ_RS04760 0.50 -1.5 893,875 + MMJJ_RS04760 0.50 -2.0 893,875 + MMJJ_RS04760 0.50 +0.5 893,880 - MMJJ_RS04760 0.50 -1.1 893,881 + MMJJ_RS04760 0.50 -4.0 893,899 - MMJJ_RS04760 0.52 -2.6 894,037 + MMJJ_RS04760 0.61 -2.3 894,037 + MMJJ_RS04760 0.61 -3.0 894,073 - MMJJ_RS04760 0.63 -1.7 894,073 - MMJJ_RS04760 0.63 -1.4 894,073 - MMJJ_RS04760 0.63 -2.2 894,073 - MMJJ_RS04760 0.63 -0.7 894,073 - MMJJ_RS04760 0.63 +0.5 894,073 - MMJJ_RS04760 0.63 -1.1 894,074 + MMJJ_RS04760 0.63 -4.1 894,229 - MMJJ_RS04760 0.73 -1.1 894,246 - MMJJ_RS04760 0.74 -1.1 894,246 - MMJJ_RS04760 0.74 -1.6 894,246 - MMJJ_RS04760 0.74 -2.4 894,246 - MMJJ_RS04760 0.74 -2.3 894,247 + MMJJ_RS04760 0.74 -0.6 894,247 + MMJJ_RS04760 0.74 -0.6 894,247 + MMJJ_RS04760 0.74 -1.6 894,247 + MMJJ_RS04760 0.74 -1.7 894,247 + MMJJ_RS04760 0.74 -2.2 894,247 + MMJJ_RS04760 0.74 -2.3 894,247 + MMJJ_RS04760 0.74 -3.0 894,247 + MMJJ_RS04760 0.74 -2.0 894,248 - MMJJ_RS04760 0.74 +0.0 894,269 - MMJJ_RS04760 0.76 -1.4 894,270 + MMJJ_RS04760 0.76 -0.8 894,276 - MMJJ_RS04760 0.76 -0.3 894,276 - MMJJ_RS04760 0.76 -1.8 894,302 + MMJJ_RS04760 0.78 -2.3 894,305 + MMJJ_RS04760 0.78 -0.8 894,317 - MMJJ_RS04760 0.79 -0.7 894,318 + MMJJ_RS04760 0.79 -1.3 894,318 + MMJJ_RS04760 0.79 -1.8 894,329 - MMJJ_RS04760 0.80 -2.2 894,329 - MMJJ_RS04760 0.80 -1.6 894,366 - MMJJ_RS04760 0.82 -1.8 894,401 - MMJJ_RS04760 0.84 -1.7 894,401 - MMJJ_RS04760 0.84 -3.6 894,401 - MMJJ_RS04760 0.84 -2.0 894,401 - MMJJ_RS04760 0.84 -0.8 894,401 - MMJJ_RS04760 0.84 -2.5 894,402 + MMJJ_RS04760 0.84 -3.7 894,402 + MMJJ_RS04760 0.84 -3.0 894,402 + MMJJ_RS04760 0.84 +2.0 894,402 + MMJJ_RS04760 0.84 -1.2 894,404 + MMJJ_RS04760 0.85 -2.8 894,444 - MMJJ_RS04760 0.87 -2.9 894,444 - MMJJ_RS04760 0.87 -1.7 894,444 - MMJJ_RS04760 0.87 -2.1 894,445 + MMJJ_RS04760 0.87 -0.9 894,445 + MMJJ_RS04760 0.87 -1.4 894,445 + MMJJ_RS04760 0.87 -1.2 894,445 + MMJJ_RS04760 0.87 -2.6 894,445 + MMJJ_RS04760 0.87 -0.1 894,445 + MMJJ_RS04760 0.87 -1.1 894,445 + MMJJ_RS04760 0.87 -1.7 894,445 + MMJJ_RS04760 0.87 -3.2 894,446 - MMJJ_RS04760 0.87 -1.2 894,446 - MMJJ_RS04760 0.87 -2.1 894,446 - MMJJ_RS04760 0.87 -2.4 894,446 - MMJJ_RS04760 0.87 -2.2 894,447 + MMJJ_RS04760 0.87 -1.7 894,447 + MMJJ_RS04760 0.87 -3.5 894,447 + MMJJ_RS04760 0.87 -2.7 894,447 + MMJJ_RS04760 0.87 -1.2 894,447 + MMJJ_RS04760 0.87 -1.8 894,447 + MMJJ_RS04760 0.87 -0.9 894,447 + MMJJ_RS04760 0.87 -1.1 894,450 - MMJJ_RS04760 0.88 -1.5 894,450 - MMJJ_RS04760 0.88 -1.6 894,450 - MMJJ_RS04760 0.88 -2.4 894,496 + -1.0 894,511 + -0.7 894,518 - -2.2 894,519 + -1.6 894,519 + -1.3 894,575 + -1.4 894,577 - -0.5 894,578 + -2.5 894,578 + -1.8 894,601 + -3.6 894,634 + -1.5 894,634 + -0.4 894,634 + -0.2 894,698 - -1.2 894,699 + -0.7 894,699 + +0.7 894,732 + -0.8 894,732 + -3.0 894,761 + MMJJ_RS04765 0.12 +0.3 894,788 + MMJJ_RS04765 0.16 -0.2 894,799 - MMJJ_RS04765 0.17 -1.2 894,812 + MMJJ_RS04765 0.19 -1.0 894,812 + MMJJ_RS04765 0.19 +0.0 894,828 - MMJJ_RS04765 0.22 +0.3 894,828 - MMJJ_RS04765 0.22 -1.1 894,828 - MMJJ_RS04765 0.22 -0.2 894,828 - MMJJ_RS04765 0.22 -1.7 894,828 - MMJJ_RS04765 0.22 -0.3 894,828 - MMJJ_RS04765 0.22 -3.2 894,829 + MMJJ_RS04765 0.22 -0.4 894,829 + MMJJ_RS04765 0.22 +1.3 894,830 - MMJJ_RS04765 0.22 -2.6 894,830 - MMJJ_RS04765 0.22 -1.3 894,830 - MMJJ_RS04765 0.22 +0.4 894,831 + MMJJ_RS04765 0.22 -2.7 894,831 + MMJJ_RS04765 0.22 -0.8 894,846 + MMJJ_RS04765 0.24 -0.4 894,867 - MMJJ_RS04765 0.28 +1.8 894,867 - MMJJ_RS04765 0.28 -0.7 894,867 - MMJJ_RS04765 0.28 -3.1 894,867 - MMJJ_RS04765 0.28 -1.3 894,871 - MMJJ_RS04765 0.28 -0.7 894,871 - MMJJ_RS04765 0.28 -3.2 894,885 - MMJJ_RS04765 0.30 +0.5 894,935 - MMJJ_RS04765 0.38 -3.1 894,949 + MMJJ_RS04765 0.40 -2.8 894,995 - MMJJ_RS04765 0.47 -0.3 895,088 - MMJJ_RS04765 0.60 -1.9 895,089 + MMJJ_RS04765 0.60 -0.6 895,160 - MMJJ_RS04765 0.71 -1.1 895,161 + MMJJ_RS04765 0.71 -1.6 895,185 - MMJJ_RS04765 0.75 -2.4 895,190 + MMJJ_RS04765 0.75 -1.2 895,209 - MMJJ_RS04765 0.78 -2.9 895,277 - MMJJ_RS04765 0.88 -1.3 895,287 + MMJJ_RS04765 0.90 -0.6 895,599 - MMJJ_RS04770 0.19 +0.7
Or see this region's nucleotide sequence