Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04695 and MMJJ_RS04700 are separated by 635 nucleotides MMJJ_RS04700 and MMJJ_RS04705 are separated by 56 nucleotides MMJJ_RS04705 and MMJJ_RS04710 are separated by 17 nucleotides
MMJJ_RS04695: MMJJ_RS04695 - formate dehydrogenase subunit alpha, at 878,542 to 880,572
_RS04695
MMJJ_RS04700: MMJJ_RS04700 - deoxyhypusine synthase, at 881,208 to 882,215
_RS04700
MMJJ_RS04705: MMJJ_RS04705 - 3-isopropylmalate dehydratase small subunit, at 882,272 to 882,775
_RS04705
MMJJ_RS04710: MMJJ_RS04710 - threonine synthase, at 882,793 to 884,007
_RS04710
Position (kb) 881
882
883 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 880.214 kb on + strand, within MMJJ_RS04695 at 880.241 kb on - strand, within MMJJ_RS04695 at 880.241 kb on - strand, within MMJJ_RS04695 at 880.241 kb on - strand, within MMJJ_RS04695 at 880.242 kb on + strand, within MMJJ_RS04695 at 880.282 kb on - strand, within MMJJ_RS04695 at 880.283 kb on + strand, within MMJJ_RS04695 at 880.299 kb on + strand, within MMJJ_RS04695 at 880.307 kb on + strand, within MMJJ_RS04695 at 880.321 kb on + strand, within MMJJ_RS04695 at 880.328 kb on + strand, within MMJJ_RS04695 at 880.344 kb on - strand, within MMJJ_RS04695 at 880.345 kb on + strand, within MMJJ_RS04695 at 880.422 kb on + strand at 880.425 kb on - strand at 880.426 kb on + strand at 880.433 kb on + strand at 880.434 kb on - strand at 880.483 kb on - strand at 880.484 kb on + strand at 880.486 kb on - strand at 880.487 kb on + strand at 880.500 kb on - strand at 880.505 kb on + strand at 880.560 kb on - strand at 880.573 kb on - strand at 880.597 kb on - strand at 880.641 kb on + strand at 880.687 kb on - strand at 880.711 kb on + strand at 880.711 kb on + strand at 880.773 kb on - strand at 880.875 kb on + strand at 880.887 kb on - strand at 880.974 kb on + strand at 881.024 kb on - strand at 881.073 kb on + strand at 881.123 kb on + strand at 881.144 kb on + strand at 881.192 kb on - strand at 881.193 kb on + strand at 881.210 kb on + strand at 881.213 kb on - strand at 881.360 kb on - strand, within MMJJ_RS04700 at 881.360 kb on - strand, within MMJJ_RS04700 at 881.361 kb on + strand, within MMJJ_RS04700 at 881.361 kb on + strand, within MMJJ_RS04700 at 881.365 kb on - strand, within MMJJ_RS04700 at 881.406 kb on - strand, within MMJJ_RS04700 at 881.410 kb on + strand at 881.508 kb on - strand, within MMJJ_RS04700 at 881.611 kb on - strand, within MMJJ_RS04700 at 881.758 kb on - strand, within MMJJ_RS04700 at 881.758 kb on - strand, within MMJJ_RS04700 at 881.759 kb on + strand, within MMJJ_RS04700 at 881.759 kb on + strand, within MMJJ_RS04700 at 881.795 kb on + strand, within MMJJ_RS04700 at 881.795 kb on + strand, within MMJJ_RS04700 at 881.797 kb on + strand, within MMJJ_RS04700 at 881.912 kb on - strand, within MMJJ_RS04700 at 881.944 kb on + strand, within MMJJ_RS04700 at 881.955 kb on - strand, within MMJJ_RS04700 at 881.996 kb on + strand, within MMJJ_RS04700 at 882.273 kb on - strand at 882.710 kb on - strand, within MMJJ_RS04705
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 880,214 + MMJJ_RS04695 0.82 -0.6 880,241 - MMJJ_RS04695 0.84 -1.2 880,241 - MMJJ_RS04695 0.84 +0.5 880,241 - MMJJ_RS04695 0.84 -0.8 880,242 + MMJJ_RS04695 0.84 -1.6 880,282 - MMJJ_RS04695 0.86 +0.6 880,283 + MMJJ_RS04695 0.86 -1.4 880,299 + MMJJ_RS04695 0.87 -0.6 880,307 + MMJJ_RS04695 0.87 -0.1 880,321 + MMJJ_RS04695 0.88 +0.4 880,328 + MMJJ_RS04695 0.88 -0.5 880,344 - MMJJ_RS04695 0.89 +1.5 880,345 + MMJJ_RS04695 0.89 -0.7 880,422 + +0.7 880,425 - +0.3 880,426 + +0.6 880,433 + -0.8 880,434 - -1.1 880,483 - +1.3 880,484 + +0.5 880,486 - +0.6 880,487 + +0.8 880,500 - +0.7 880,505 + -0.3 880,560 - +0.9 880,573 - -0.7 880,597 - +1.4 880,641 + +0.2 880,687 - -1.4 880,711 + -0.4 880,711 + +1.1 880,773 - +0.8 880,875 + -0.2 880,887 - -0.2 880,974 + -0.4 881,024 - -2.6 881,073 + +2.6 881,123 + +0.9 881,144 + -0.8 881,192 - -0.4 881,193 + +0.9 881,210 + +0.0 881,213 - +0.5 881,360 - MMJJ_RS04700 0.15 -1.0 881,360 - MMJJ_RS04700 0.15 +0.0 881,361 + MMJJ_RS04700 0.15 -3.1 881,361 + MMJJ_RS04700 0.15 -1.6 881,365 - MMJJ_RS04700 0.16 -2.3 881,406 - MMJJ_RS04700 0.20 +0.2 881,410 + +0.0 881,508 - MMJJ_RS04700 0.30 -1.9 881,611 - MMJJ_RS04700 0.40 -1.9 881,758 - MMJJ_RS04700 0.55 -1.8 881,758 - MMJJ_RS04700 0.55 -1.0 881,759 + MMJJ_RS04700 0.55 -2.4 881,759 + MMJJ_RS04700 0.55 -1.6 881,795 + MMJJ_RS04700 0.58 -2.6 881,795 + MMJJ_RS04700 0.58 -1.0 881,797 + MMJJ_RS04700 0.58 -2.1 881,912 - MMJJ_RS04700 0.70 -1.9 881,944 + MMJJ_RS04700 0.73 -3.0 881,955 - MMJJ_RS04700 0.74 -2.6 881,996 + MMJJ_RS04700 0.78 -1.8 882,273 - -0.9 882,710 - MMJJ_RS04705 0.87 -2.6
Or see this region's nucleotide sequence