Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04515 and MMJJ_RS04520 overlap by 4 nucleotides MMJJ_RS04520 and MMJJ_RS04525 are separated by 81 nucleotides MMJJ_RS04525 and MMJJ_RS04530 are separated by 2 nucleotides
MMJJ_RS04515: MMJJ_RS04515 - HD domain-containing protein, at 842,549 to 843,925
_RS04515
MMJJ_RS04520: MMJJ_RS04520 - coenzyme F430 synthase, at 843,922 to 845,124
_RS04520
MMJJ_RS04525: MMJJ_RS04525 - hypothetical protein, at 845,206 to 845,490
_RS04525
MMJJ_RS04530: MMJJ_RS04530 - iron export ABC transporter permease subunit FetB, at 845,493 to 846,239
_RS04530
Position (kb) 843
844
845
846 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 842.925 kb on - strand, within MMJJ_RS04515 at 842.933 kb on - strand, within MMJJ_RS04515 at 842.996 kb on - strand, within MMJJ_RS04515 at 842.997 kb on + strand, within MMJJ_RS04515 at 842.997 kb on + strand, within MMJJ_RS04515 at 843.000 kb on - strand, within MMJJ_RS04515 at 843.001 kb on + strand, within MMJJ_RS04515 at 843.001 kb on + strand, within MMJJ_RS04515 at 843.001 kb on + strand, within MMJJ_RS04515 at 843.003 kb on - strand, within MMJJ_RS04515 at 843.042 kb on - strand, within MMJJ_RS04515 at 843.042 kb on - strand, within MMJJ_RS04515 at 843.042 kb on - strand, within MMJJ_RS04515 at 843.043 kb on + strand, within MMJJ_RS04515 at 843.047 kb on + strand, within MMJJ_RS04515 at 843.067 kb on - strand, within MMJJ_RS04515 at 843.067 kb on - strand, within MMJJ_RS04515 at 843.068 kb on + strand, within MMJJ_RS04515 at 843.150 kb on + strand, within MMJJ_RS04515 at 843.170 kb on - strand, within MMJJ_RS04515 at 843.171 kb on + strand, within MMJJ_RS04515 at 843.171 kb on + strand, within MMJJ_RS04515 at 843.171 kb on + strand, within MMJJ_RS04515 at 843.171 kb on + strand, within MMJJ_RS04515 at 843.171 kb on + strand, within MMJJ_RS04515 at 843.172 kb on - strand, within MMJJ_RS04515 at 843.173 kb on + strand, within MMJJ_RS04515 at 843.173 kb on + strand, within MMJJ_RS04515 at 843.232 kb on - strand, within MMJJ_RS04515 at 843.252 kb on - strand, within MMJJ_RS04515 at 843.262 kb on + strand, within MMJJ_RS04515 at 843.267 kb on - strand, within MMJJ_RS04515 at 843.286 kb on + strand, within MMJJ_RS04515 at 843.286 kb on + strand, within MMJJ_RS04515 at 843.288 kb on - strand, within MMJJ_RS04515 at 843.289 kb on + strand, within MMJJ_RS04515 at 843.289 kb on + strand, within MMJJ_RS04515 at 843.292 kb on - strand, within MMJJ_RS04515 at 843.292 kb on - strand, within MMJJ_RS04515 at 843.292 kb on - strand, within MMJJ_RS04515 at 843.293 kb on + strand, within MMJJ_RS04515 at 843.293 kb on + strand, within MMJJ_RS04515 at 843.293 kb on + strand, within MMJJ_RS04515 at 843.293 kb on + strand, within MMJJ_RS04515 at 843.297 kb on - strand, within MMJJ_RS04515 at 843.298 kb on + strand, within MMJJ_RS04515 at 843.329 kb on + strand, within MMJJ_RS04515 at 843.339 kb on - strand, within MMJJ_RS04515 at 843.354 kb on - strand, within MMJJ_RS04515 at 843.359 kb on + strand, within MMJJ_RS04515 at 843.365 kb on + strand, within MMJJ_RS04515 at 843.381 kb on + strand, within MMJJ_RS04515 at 843.381 kb on + strand, within MMJJ_RS04515 at 843.381 kb on + strand, within MMJJ_RS04515 at 843.381 kb on + strand, within MMJJ_RS04515 at 843.494 kb on + strand, within MMJJ_RS04515 at 843.529 kb on - strand, within MMJJ_RS04515 at 843.530 kb on + strand, within MMJJ_RS04515 at 843.530 kb on + strand, within MMJJ_RS04515 at 843.537 kb on - strand, within MMJJ_RS04515 at 843.603 kb on + strand, within MMJJ_RS04515 at 843.633 kb on + strand, within MMJJ_RS04515 at 843.724 kb on - strand, within MMJJ_RS04515 at 843.725 kb on + strand, within MMJJ_RS04515 at 843.888 kb on + strand at 843.888 kb on + strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.889 kb on - strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.890 kb on + strand at 843.893 kb on - strand at 843.893 kb on - strand at 843.893 kb on - strand at 843.894 kb on + strand at 843.902 kb on + strand at 843.902 kb on + strand at 843.902 kb on + strand at 843.902 kb on + strand at 843.941 kb on - strand at 843.941 kb on - strand at 843.954 kb on - strand at 843.971 kb on - strand at 843.972 kb on + strand at 843.999 kb on - strand at 844.047 kb on - strand, within MMJJ_RS04520 at 844.048 kb on + strand, within MMJJ_RS04520 at 844.100 kb on + strand, within MMJJ_RS04520 at 844.138 kb on - strand, within MMJJ_RS04520 at 844.295 kb on + strand, within MMJJ_RS04520 at 844.316 kb on - strand, within MMJJ_RS04520 at 844.324 kb on - strand, within MMJJ_RS04520 at 844.324 kb on - strand, within MMJJ_RS04520 at 844.324 kb on - strand at 844.325 kb on + strand, within MMJJ_RS04520 at 844.558 kb on + strand, within MMJJ_RS04520 at 844.575 kb on + strand, within MMJJ_RS04520 at 844.690 kb on - strand, within MMJJ_RS04520 at 844.729 kb on + strand, within MMJJ_RS04520 at 844.732 kb on + strand, within MMJJ_RS04520 at 844.786 kb on + strand, within MMJJ_RS04520 at 844.935 kb on + strand, within MMJJ_RS04520 at 845.092 kb on - strand at 845.147 kb on + strand at 845.163 kb on - strand at 845.213 kb on - strand at 845.213 kb on - strand at 845.213 kb on - strand at 845.213 kb on - strand at 845.213 kb on - strand at 845.213 kb on - strand at 845.214 kb on + strand at 845.214 kb on + strand at 845.214 kb on + strand at 845.228 kb on - strand at 845.231 kb on - strand at 845.231 kb on - strand at 845.231 kb on - strand at 845.232 kb on + strand at 845.236 kb on + strand, within MMJJ_RS04525 at 845.238 kb on - strand, within MMJJ_RS04525 at 845.238 kb on - strand, within MMJJ_RS04525 at 845.239 kb on + strand, within MMJJ_RS04525 at 845.359 kb on + strand, within MMJJ_RS04525 at 845.385 kb on - strand, within MMJJ_RS04525 at 845.468 kb on - strand at 845.513 kb on - strand at 845.514 kb on + strand at 845.525 kb on + strand at 845.533 kb on - strand at 845.561 kb on - strand at 845.562 kb on + strand at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.585 kb on - strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.586 kb on + strand, within MMJJ_RS04530 at 845.590 kb on + strand, within MMJJ_RS04530 at 845.611 kb on + strand, within MMJJ_RS04530 at 845.657 kb on - strand, within MMJJ_RS04530 at 845.658 kb on + strand, within MMJJ_RS04530 at 845.689 kb on + strand, within MMJJ_RS04530 at 845.733 kb on + strand, within MMJJ_RS04530 at 845.826 kb on - strand, within MMJJ_RS04530 at 845.828 kb on - strand, within MMJJ_RS04530 at 845.829 kb on + strand, within MMJJ_RS04530 at 845.877 kb on - strand, within MMJJ_RS04530 at 845.878 kb on + strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.895 kb on - strand, within MMJJ_RS04530 at 845.946 kb on + strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.970 kb on - strand, within MMJJ_RS04530 at 845.973 kb on - strand, within MMJJ_RS04530 at 846.039 kb on - strand, within MMJJ_RS04530 at 846.040 kb on + strand, within MMJJ_RS04530 at 846.043 kb on + strand, within MMJJ_RS04530 at 846.043 kb on + strand, within MMJJ_RS04530 at 846.047 kb on - strand, within MMJJ_RS04530 at 846.047 kb on - strand, within MMJJ_RS04530 at 846.048 kb on + strand, within MMJJ_RS04530 at 846.074 kb on - strand, within MMJJ_RS04530
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 842,925 - MMJJ_RS04515 0.27 +1.4 842,933 - MMJJ_RS04515 0.28 -1.1 842,996 - MMJJ_RS04515 0.32 +1.5 842,997 + MMJJ_RS04515 0.33 +1.6 842,997 + MMJJ_RS04515 0.33 -0.5 843,000 - MMJJ_RS04515 0.33 -0.5 843,001 + MMJJ_RS04515 0.33 +1.0 843,001 + MMJJ_RS04515 0.33 -0.8 843,001 + MMJJ_RS04515 0.33 +0.5 843,003 - MMJJ_RS04515 0.33 -0.5 843,042 - MMJJ_RS04515 0.36 +0.6 843,042 - MMJJ_RS04515 0.36 -0.5 843,042 - MMJJ_RS04515 0.36 -0.1 843,043 + MMJJ_RS04515 0.36 +1.2 843,047 + MMJJ_RS04515 0.36 +1.7 843,067 - MMJJ_RS04515 0.38 +1.6 843,067 - MMJJ_RS04515 0.38 -0.4 843,068 + MMJJ_RS04515 0.38 -0.5 843,150 + MMJJ_RS04515 0.44 +1.2 843,170 - MMJJ_RS04515 0.45 -0.2 843,171 + MMJJ_RS04515 0.45 -1.0 843,171 + MMJJ_RS04515 0.45 -0.2 843,171 + MMJJ_RS04515 0.45 +1.0 843,171 + MMJJ_RS04515 0.45 -0.3 843,171 + MMJJ_RS04515 0.45 +0.0 843,172 - MMJJ_RS04515 0.45 +1.0 843,173 + MMJJ_RS04515 0.45 -0.4 843,173 + MMJJ_RS04515 0.45 -0.0 843,232 - MMJJ_RS04515 0.50 +0.7 843,252 - MMJJ_RS04515 0.51 -1.1 843,262 + MMJJ_RS04515 0.52 +0.0 843,267 - MMJJ_RS04515 0.52 -1.0 843,286 + MMJJ_RS04515 0.54 -1.4 843,286 + MMJJ_RS04515 0.54 +0.7 843,288 - MMJJ_RS04515 0.54 +0.3 843,289 + MMJJ_RS04515 0.54 +0.0 843,289 + MMJJ_RS04515 0.54 -0.0 843,292 - MMJJ_RS04515 0.54 -0.4 843,292 - MMJJ_RS04515 0.54 +1.3 843,292 - MMJJ_RS04515 0.54 -0.6 843,293 + MMJJ_RS04515 0.54 -0.6 843,293 + MMJJ_RS04515 0.54 +0.5 843,293 + MMJJ_RS04515 0.54 +1.8 843,293 + MMJJ_RS04515 0.54 +0.7 843,297 - MMJJ_RS04515 0.54 -1.8 843,298 + MMJJ_RS04515 0.54 +1.9 843,329 + MMJJ_RS04515 0.57 +2.0 843,339 - MMJJ_RS04515 0.57 +0.0 843,354 - MMJJ_RS04515 0.58 +0.1 843,359 + MMJJ_RS04515 0.59 -0.7 843,365 + MMJJ_RS04515 0.59 +0.5 843,381 + MMJJ_RS04515 0.60 -1.0 843,381 + MMJJ_RS04515 0.60 +0.9 843,381 + MMJJ_RS04515 0.60 -0.4 843,381 + MMJJ_RS04515 0.60 +0.9 843,494 + MMJJ_RS04515 0.69 +1.0 843,529 - MMJJ_RS04515 0.71 -1.3 843,530 + MMJJ_RS04515 0.71 -1.1 843,530 + MMJJ_RS04515 0.71 +0.4 843,537 - MMJJ_RS04515 0.72 -0.2 843,603 + MMJJ_RS04515 0.77 -2.6 843,633 + MMJJ_RS04515 0.79 +0.7 843,724 - MMJJ_RS04515 0.85 -0.6 843,725 + MMJJ_RS04515 0.85 -0.6 843,888 + +1.0 843,888 + -1.3 843,889 - +0.1 843,889 - +1.1 843,889 - +1.2 843,889 - +0.0 843,889 - +1.1 843,889 - +1.4 843,889 - +0.2 843,889 - +0.2 843,889 - +0.2 843,889 - +1.1 843,889 - -0.7 843,889 - -0.2 843,889 - +1.0 843,890 + +1.0 843,890 + +1.8 843,890 + +1.0 843,890 + -0.2 843,890 + -0.5 843,890 + +1.0 843,890 + +0.0 843,890 + -0.4 843,890 + -0.1 843,890 + +0.7 843,893 - +0.9 843,893 - +1.7 843,893 - -1.1 843,894 + -0.2 843,902 + +0.7 843,902 + +0.6 843,902 + -0.9 843,902 + +1.1 843,941 - -1.2 843,941 - -2.4 843,954 - -3.5 843,971 - -1.9 843,972 + -3.2 843,999 - -1.5 844,047 - MMJJ_RS04520 0.10 -1.8 844,048 + MMJJ_RS04520 0.10 -2.8 844,100 + MMJJ_RS04520 0.15 -2.7 844,138 - MMJJ_RS04520 0.18 -1.3 844,295 + MMJJ_RS04520 0.31 -3.0 844,316 - MMJJ_RS04520 0.33 -4.4 844,324 - MMJJ_RS04520 0.33 -2.8 844,324 - MMJJ_RS04520 0.33 -4.0 844,324 - -0.5 844,325 + MMJJ_RS04520 0.33 -3.3 844,558 + MMJJ_RS04520 0.53 -2.9 844,575 + MMJJ_RS04520 0.54 -3.2 844,690 - MMJJ_RS04520 0.64 -1.9 844,729 + MMJJ_RS04520 0.67 -1.8 844,732 + MMJJ_RS04520 0.67 -3.8 844,786 + MMJJ_RS04520 0.72 -1.8 844,935 + MMJJ_RS04520 0.84 -2.7 845,092 - -4.0 845,147 + +0.9 845,163 - +1.5 845,213 - -0.6 845,213 - +0.2 845,213 - +1.4 845,213 - +0.6 845,213 - -0.8 845,213 - -1.3 845,214 + +0.4 845,214 + +1.1 845,214 + -1.2 845,228 - -1.4 845,231 - +0.3 845,231 - -1.0 845,231 - -0.6 845,232 + +0.8 845,236 + MMJJ_RS04525 0.11 +1.2 845,238 - MMJJ_RS04525 0.11 +1.3 845,238 - MMJJ_RS04525 0.11 +0.8 845,239 + MMJJ_RS04525 0.12 -0.3 845,359 + MMJJ_RS04525 0.54 +1.2 845,385 - MMJJ_RS04525 0.63 +1.1 845,468 - +0.5 845,513 - +0.8 845,514 + +1.0 845,525 + +0.3 845,533 - +0.3 845,561 - -0.5 845,562 + +0.9 845,585 - MMJJ_RS04530 0.12 +0.6 845,585 - MMJJ_RS04530 0.12 +0.0 845,585 - MMJJ_RS04530 0.12 -0.1 845,585 - MMJJ_RS04530 0.12 +1.0 845,585 - MMJJ_RS04530 0.12 -0.1 845,586 + MMJJ_RS04530 0.12 -1.0 845,586 + MMJJ_RS04530 0.12 +0.5 845,586 + MMJJ_RS04530 0.12 +0.9 845,590 + MMJJ_RS04530 0.13 +1.1 845,611 + MMJJ_RS04530 0.16 -0.5 845,657 - MMJJ_RS04530 0.22 -1.1 845,658 + MMJJ_RS04530 0.22 +0.3 845,689 + MMJJ_RS04530 0.26 -0.8 845,733 + MMJJ_RS04530 0.32 -2.3 845,826 - MMJJ_RS04530 0.45 -0.5 845,828 - MMJJ_RS04530 0.45 +0.5 845,829 + MMJJ_RS04530 0.45 -0.0 845,877 - MMJJ_RS04530 0.51 -1.3 845,878 + MMJJ_RS04530 0.52 +1.1 845,895 - MMJJ_RS04530 0.54 -0.8 845,895 - MMJJ_RS04530 0.54 -0.8 845,895 - MMJJ_RS04530 0.54 -0.4 845,946 + MMJJ_RS04530 0.61 +0.4 845,970 - MMJJ_RS04530 0.64 -0.3 845,970 - MMJJ_RS04530 0.64 +0.0 845,970 - MMJJ_RS04530 0.64 -0.1 845,973 - MMJJ_RS04530 0.64 +0.6 846,039 - MMJJ_RS04530 0.73 -0.4 846,040 + MMJJ_RS04530 0.73 -0.8 846,043 + MMJJ_RS04530 0.74 +0.7 846,043 + MMJJ_RS04530 0.74 -0.5 846,047 - MMJJ_RS04530 0.74 -0.1 846,047 - MMJJ_RS04530 0.74 +0.1 846,048 + MMJJ_RS04530 0.74 +0.2 846,074 - MMJJ_RS04530 0.78 +0.4
Or see this region's nucleotide sequence