Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04475 and MMJJ_RS04480 are separated by 170 nucleotides MMJJ_RS04480 and MMJJ_RS04485 are separated by 143 nucleotides MMJJ_RS04485 and MMJJ_RS04490 are separated by 172 nucleotides
MMJJ_RS04475: MMJJ_RS04475 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 833,242 to 833,985
_RS04475
MMJJ_RS04480: MMJJ_RS04480 - hypothetical protein, at 834,156 to 834,368
_RS04480
MMJJ_RS04485: MMJJ_RS04485 - riboflavin synthase, at 834,512 to 834,982
_RS04485
MMJJ_RS04490: MMJJ_RS04490 - AIR synthase-related protein, at 835,155 to 838,124
_RS04490
Position (kb) 834
835 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 833.565 kb on + strand, within MMJJ_RS04475 at 833.565 kb on + strand, within MMJJ_RS04475 at 833.566 kb on - strand, within MMJJ_RS04475 at 833.649 kb on - strand, within MMJJ_RS04475 at 833.650 kb on + strand, within MMJJ_RS04475 at 833.760 kb on - strand, within MMJJ_RS04475 at 833.841 kb on - strand at 833.929 kb on + strand at 834.068 kb on - strand at 834.069 kb on + strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.071 kb on + strand at 834.071 kb on + strand at 834.104 kb on + strand at 834.135 kb on + strand at 834.135 kb on + strand at 834.139 kb on + strand at 834.155 kb on - strand at 834.164 kb on - strand at 834.179 kb on - strand, within MMJJ_RS04480 at 834.179 kb on - strand, within MMJJ_RS04480 at 834.181 kb on - strand, within MMJJ_RS04480 at 834.276 kb on - strand, within MMJJ_RS04480 at 834.278 kb on - strand, within MMJJ_RS04480 at 834.350 kb on - strand at 834.395 kb on + strand at 834.395 kb on + strand at 834.397 kb on - strand at 834.397 kb on - strand at 834.414 kb on - strand at 834.462 kb on + strand at 834.467 kb on + strand at 834.470 kb on - strand at 834.470 kb on - strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.485 kb on - strand at 834.486 kb on + strand at 834.486 kb on + strand at 834.525 kb on - strand at 834.525 kb on - strand at 834.605 kb on + strand, within MMJJ_RS04485 at 834.613 kb on + strand, within MMJJ_RS04485 at 834.621 kb on + strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.843 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.904 kb on - strand, within MMJJ_RS04485 at 835.034 kb on - strand at 835.034 kb on - strand at 835.034 kb on - strand at 835.035 kb on + strand at 835.035 kb on + strand at 835.039 kb on - strand at 835.043 kb on - strand at 835.043 kb on - strand at 835.044 kb on + strand at 835.064 kb on - strand at 835.065 kb on + strand at 835.070 kb on - strand at 835.081 kb on + strand at 835.969 kb on - strand, within MMJJ_RS04490
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 833,565 + MMJJ_RS04475 0.43 -2.0 833,565 + MMJJ_RS04475 0.43 -3.1 833,566 - MMJJ_RS04475 0.44 -3.6 833,649 - MMJJ_RS04475 0.55 -2.4 833,650 + MMJJ_RS04475 0.55 -1.8 833,760 - MMJJ_RS04475 0.70 -2.0 833,841 - -0.5 833,929 + -3.6 834,068 - +1.0 834,069 + +0.2 834,070 - -0.1 834,070 - -1.0 834,070 - -1.2 834,071 + -0.1 834,071 + +0.9 834,104 + +0.1 834,135 + -0.4 834,135 + -0.2 834,139 + -0.7 834,155 - +0.8 834,164 - +0.8 834,179 - MMJJ_RS04480 0.11 +0.9 834,179 - MMJJ_RS04480 0.11 +0.5 834,181 - MMJJ_RS04480 0.12 -1.3 834,276 - MMJJ_RS04480 0.56 +0.2 834,278 - MMJJ_RS04480 0.57 +0.3 834,350 - -1.5 834,395 + +2.1 834,395 + +1.3 834,397 - +1.5 834,397 - -0.2 834,414 - +0.5 834,462 + -3.1 834,467 + -2.9 834,470 - -1.3 834,470 - -3.5 834,476 + -3.4 834,476 + -1.7 834,476 + -2.8 834,476 + -2.9 834,476 + -2.5 834,485 - -0.3 834,486 + -4.0 834,486 + -3.7 834,525 - -1.5 834,525 - +0.5 834,605 + MMJJ_RS04485 0.20 -3.2 834,613 + MMJJ_RS04485 0.21 -3.9 834,621 + MMJJ_RS04485 0.23 -4.7 834,691 - MMJJ_RS04485 0.38 -2.0 834,691 - MMJJ_RS04485 0.38 -3.1 834,794 + MMJJ_RS04485 0.60 -3.0 834,794 + MMJJ_RS04485 0.60 +0.5 834,843 - MMJJ_RS04485 0.70 -4.0 834,888 - MMJJ_RS04485 0.80 +0.0 834,888 - MMJJ_RS04485 0.80 -5.8 834,893 - MMJJ_RS04485 0.81 -3.9 834,893 - MMJJ_RS04485 0.81 -2.4 834,893 - MMJJ_RS04485 0.81 -3.4 834,893 - MMJJ_RS04485 0.81 -2.5 834,894 + MMJJ_RS04485 0.81 -4.3 834,894 + MMJJ_RS04485 0.81 -2.5 834,894 + MMJJ_RS04485 0.81 -4.5 834,894 + MMJJ_RS04485 0.81 -4.6 834,894 + MMJJ_RS04485 0.81 -4.6 834,904 - MMJJ_RS04485 0.83 -2.7 835,034 - +0.9 835,034 - +1.1 835,034 - -2.2 835,035 + +0.6 835,035 + -0.4 835,039 - -1.0 835,043 - -1.3 835,043 - -1.2 835,044 + +0.7 835,064 - -0.5 835,065 + -0.6 835,070 - -0.5 835,081 + -2.7 835,969 - MMJJ_RS04490 0.27 -2.0
Or see this region's nucleotide sequence