Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04465 and MMJJ_RS04470 are separated by 108 nucleotides MMJJ_RS04470 and MMJJ_RS04475 are separated by 120 nucleotides MMJJ_RS04475 and MMJJ_RS04480 are separated by 170 nucleotides MMJJ_RS04480 and MMJJ_RS04485 are separated by 143 nucleotides
MMJJ_RS04465: MMJJ_RS04465 - MTAP family purine nucleoside phosphorylase, at 831,868 to 832,629
_RS04465
MMJJ_RS04470: MMJJ_RS04470 - CTP-dependent riboflavin kinase, at 832,738 to 833,121
_RS04470
MMJJ_RS04475: MMJJ_RS04475 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 833,242 to 833,985
_RS04475
MMJJ_RS04480: MMJJ_RS04480 - hypothetical protein, at 834,156 to 834,368
_RS04480
MMJJ_RS04485: MMJJ_RS04485 - riboflavin synthase, at 834,512 to 834,982
_RS04485
Position (kb) 833
834 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 832.243 kb on - strand, within MMJJ_RS04465 at 832.243 kb on - strand, within MMJJ_RS04465 at 832.244 kb on + strand, within MMJJ_RS04465 at 832.256 kb on - strand, within MMJJ_RS04465 at 832.268 kb on + strand, within MMJJ_RS04465 at 832.268 kb on + strand, within MMJJ_RS04465 at 832.305 kb on + strand, within MMJJ_RS04465 at 832.329 kb on - strand, within MMJJ_RS04465 at 832.330 kb on + strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.372 kb on - strand, within MMJJ_RS04465 at 832.373 kb on + strand, within MMJJ_RS04465 at 832.373 kb on + strand, within MMJJ_RS04465 at 832.384 kb on - strand, within MMJJ_RS04465 at 832.385 kb on + strand, within MMJJ_RS04465 at 832.385 kb on + strand, within MMJJ_RS04465 at 832.402 kb on - strand, within MMJJ_RS04465 at 832.403 kb on + strand, within MMJJ_RS04465 at 832.403 kb on + strand, within MMJJ_RS04465 at 832.412 kb on + strand, within MMJJ_RS04465 at 832.435 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.462 kb on - strand, within MMJJ_RS04465 at 832.504 kb on - strand, within MMJJ_RS04465 at 832.504 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.513 kb on - strand, within MMJJ_RS04465 at 832.514 kb on + strand, within MMJJ_RS04465 at 832.535 kb on + strand, within MMJJ_RS04465 at 832.543 kb on + strand, within MMJJ_RS04465 at 832.568 kb on + strand at 832.588 kb on - strand at 832.589 kb on + strand at 832.589 kb on + strand at 832.589 kb on + strand at 832.591 kb on - strand at 832.632 kb on + strand at 832.652 kb on - strand at 832.652 kb on - strand at 832.652 kb on - strand at 832.656 kb on - strand at 832.657 kb on + strand at 832.677 kb on - strand at 832.677 kb on - strand at 832.682 kb on + strand at 832.694 kb on - strand at 832.717 kb on - strand at 832.718 kb on + strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.719 kb on - strand at 832.720 kb on + strand at 832.720 kb on + strand at 832.720 kb on + strand at 832.721 kb on - strand at 833.084 kb on - strand at 833.119 kb on - strand at 833.120 kb on + strand at 833.125 kb on + strand at 833.128 kb on - strand at 833.129 kb on + strand at 833.129 kb on + strand at 833.162 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.189 kb on - strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.190 kb on + strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.191 kb on - strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.192 kb on + strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.193 kb on - strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.194 kb on + strand at 833.200 kb on + strand at 833.227 kb on - strand at 833.228 kb on + strand at 833.230 kb on - strand at 833.230 kb on - strand at 833.231 kb on + strand at 833.247 kb on - strand at 833.286 kb on + strand at 833.416 kb on + strand, within MMJJ_RS04475 at 833.416 kb on + strand, within MMJJ_RS04475 at 833.425 kb on - strand, within MMJJ_RS04475 at 833.511 kb on - strand at 833.565 kb on + strand, within MMJJ_RS04475 at 833.565 kb on + strand, within MMJJ_RS04475 at 833.566 kb on - strand, within MMJJ_RS04475 at 833.649 kb on - strand, within MMJJ_RS04475 at 833.650 kb on + strand, within MMJJ_RS04475 at 833.760 kb on - strand, within MMJJ_RS04475 at 833.841 kb on - strand at 833.929 kb on + strand at 834.068 kb on - strand at 834.069 kb on + strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.070 kb on - strand at 834.071 kb on + strand at 834.071 kb on + strand at 834.104 kb on + strand at 834.135 kb on + strand at 834.135 kb on + strand at 834.139 kb on + strand at 834.155 kb on - strand at 834.164 kb on - strand at 834.179 kb on - strand, within MMJJ_RS04480 at 834.179 kb on - strand, within MMJJ_RS04480 at 834.181 kb on - strand, within MMJJ_RS04480 at 834.276 kb on - strand, within MMJJ_RS04480 at 834.278 kb on - strand, within MMJJ_RS04480 at 834.350 kb on - strand at 834.395 kb on + strand at 834.395 kb on + strand at 834.397 kb on - strand at 834.397 kb on - strand at 834.414 kb on - strand at 834.462 kb on + strand at 834.467 kb on + strand at 834.470 kb on - strand at 834.470 kb on - strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.476 kb on + strand at 834.485 kb on - strand at 834.486 kb on + strand at 834.486 kb on + strand at 834.525 kb on - strand at 834.525 kb on - strand at 834.605 kb on + strand, within MMJJ_RS04485 at 834.613 kb on + strand, within MMJJ_RS04485 at 834.621 kb on + strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.691 kb on - strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.794 kb on + strand, within MMJJ_RS04485 at 834.843 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.888 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.893 kb on - strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.894 kb on + strand, within MMJJ_RS04485 at 834.904 kb on - strand, within MMJJ_RS04485
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 832,243 - MMJJ_RS04465 0.49 +1.0 832,243 - MMJJ_RS04465 0.49 -1.4 832,244 + MMJJ_RS04465 0.49 +0.2 832,256 - MMJJ_RS04465 0.51 -2.5 832,268 + MMJJ_RS04465 0.52 -0.8 832,268 + MMJJ_RS04465 0.52 -0.2 832,305 + MMJJ_RS04465 0.57 -2.7 832,329 - MMJJ_RS04465 0.60 -1.1 832,330 + MMJJ_RS04465 0.61 -1.3 832,372 - MMJJ_RS04465 0.66 -0.2 832,372 - MMJJ_RS04465 0.66 -1.6 832,372 - MMJJ_RS04465 0.66 -1.5 832,372 - MMJJ_RS04465 0.66 -2.9 832,373 + MMJJ_RS04465 0.66 -0.3 832,373 + MMJJ_RS04465 0.66 +0.6 832,384 - MMJJ_RS04465 0.68 -1.0 832,385 + MMJJ_RS04465 0.68 -1.0 832,385 + MMJJ_RS04465 0.68 -1.5 832,402 - MMJJ_RS04465 0.70 -1.3 832,403 + MMJJ_RS04465 0.70 -0.8 832,403 + MMJJ_RS04465 0.70 +0.4 832,412 + MMJJ_RS04465 0.71 -0.3 832,435 - MMJJ_RS04465 0.74 -0.2 832,462 - MMJJ_RS04465 0.78 -0.5 832,462 - MMJJ_RS04465 0.78 +0.0 832,462 - MMJJ_RS04465 0.78 -1.6 832,504 - MMJJ_RS04465 0.83 -0.0 832,504 - MMJJ_RS04465 0.83 -0.5 832,513 - MMJJ_RS04465 0.85 +0.2 832,513 - MMJJ_RS04465 0.85 +0.4 832,513 - MMJJ_RS04465 0.85 +0.2 832,513 - MMJJ_RS04465 0.85 -2.8 832,514 + MMJJ_RS04465 0.85 +0.4 832,535 + MMJJ_RS04465 0.88 +0.1 832,543 + MMJJ_RS04465 0.89 -1.6 832,568 + +0.9 832,588 - +0.1 832,589 + +1.0 832,589 + -0.1 832,589 + +0.4 832,591 - +0.6 832,632 + +0.0 832,652 - +0.8 832,652 - -1.8 832,652 - -0.9 832,656 - +0.8 832,657 + -0.4 832,677 - -0.7 832,677 - +0.1 832,682 + +0.1 832,694 - -0.5 832,717 - -2.0 832,718 + -2.2 832,719 - +0.0 832,719 - -0.5 832,719 - -2.0 832,719 - -0.1 832,719 - -1.8 832,720 + -2.1 832,720 + -2.1 832,720 + -1.3 832,721 - -2.7 833,084 - -3.0 833,119 - -1.7 833,120 + +0.1 833,125 + +1.4 833,128 - -0.2 833,129 + -0.4 833,129 + +0.7 833,162 - +1.1 833,189 - -1.6 833,189 - -0.0 833,189 - +0.7 833,189 - +0.7 833,189 - -0.5 833,189 - +2.4 833,189 - +0.0 833,189 - -0.7 833,189 - -0.3 833,189 - +0.3 833,190 + +1.0 833,190 + +0.7 833,190 + +0.2 833,190 + -0.8 833,190 + +0.4 833,190 + -0.3 833,190 + -3.7 833,190 + -1.5 833,191 - +0.0 833,191 - -2.1 833,191 - -1.5 833,191 - -1.5 833,191 - +1.3 833,191 - -0.7 833,191 - -0.1 833,192 + -0.8 833,192 + -0.4 833,192 + +1.2 833,192 + +0.8 833,192 + +0.1 833,192 + -0.8 833,192 + -1.5 833,192 + -1.0 833,192 + +0.2 833,192 + +1.4 833,192 + -0.2 833,193 - +0.3 833,193 - +0.0 833,193 - -1.0 833,193 - -0.3 833,193 - +1.1 833,194 + -1.3 833,194 + +0.4 833,194 + +1.0 833,194 + +0.0 833,194 + -1.0 833,194 + -0.2 833,194 + -0.1 833,194 + +0.9 833,194 + -0.5 833,200 + -0.5 833,227 - -3.1 833,228 + -0.7 833,230 - -2.6 833,230 - -1.8 833,231 + -2.5 833,247 - -4.6 833,286 + -4.5 833,416 + MMJJ_RS04475 0.23 -3.9 833,416 + MMJJ_RS04475 0.23 -2.8 833,425 - MMJJ_RS04475 0.25 -0.8 833,511 - -1.0 833,565 + MMJJ_RS04475 0.43 -2.0 833,565 + MMJJ_RS04475 0.43 -3.1 833,566 - MMJJ_RS04475 0.44 -3.6 833,649 - MMJJ_RS04475 0.55 -2.4 833,650 + MMJJ_RS04475 0.55 -1.8 833,760 - MMJJ_RS04475 0.70 -2.0 833,841 - -0.5 833,929 + -3.6 834,068 - +1.0 834,069 + +0.2 834,070 - -0.1 834,070 - -1.0 834,070 - -1.2 834,071 + -0.1 834,071 + +0.9 834,104 + +0.1 834,135 + -0.4 834,135 + -0.2 834,139 + -0.7 834,155 - +0.8 834,164 - +0.8 834,179 - MMJJ_RS04480 0.11 +0.9 834,179 - MMJJ_RS04480 0.11 +0.5 834,181 - MMJJ_RS04480 0.12 -1.3 834,276 - MMJJ_RS04480 0.56 +0.2 834,278 - MMJJ_RS04480 0.57 +0.3 834,350 - -1.5 834,395 + +2.1 834,395 + +1.3 834,397 - +1.5 834,397 - -0.2 834,414 - +0.5 834,462 + -3.1 834,467 + -2.9 834,470 - -1.3 834,470 - -3.5 834,476 + -3.4 834,476 + -1.7 834,476 + -2.8 834,476 + -2.9 834,476 + -2.5 834,485 - -0.3 834,486 + -4.0 834,486 + -3.7 834,525 - -1.5 834,525 - +0.5 834,605 + MMJJ_RS04485 0.20 -3.2 834,613 + MMJJ_RS04485 0.21 -3.9 834,621 + MMJJ_RS04485 0.23 -4.7 834,691 - MMJJ_RS04485 0.38 -2.0 834,691 - MMJJ_RS04485 0.38 -3.1 834,794 + MMJJ_RS04485 0.60 -3.0 834,794 + MMJJ_RS04485 0.60 +0.5 834,843 - MMJJ_RS04485 0.70 -4.0 834,888 - MMJJ_RS04485 0.80 +0.0 834,888 - MMJJ_RS04485 0.80 -5.8 834,893 - MMJJ_RS04485 0.81 -3.9 834,893 - MMJJ_RS04485 0.81 -2.4 834,893 - MMJJ_RS04485 0.81 -3.4 834,893 - MMJJ_RS04485 0.81 -2.5 834,894 + MMJJ_RS04485 0.81 -4.3 834,894 + MMJJ_RS04485 0.81 -2.5 834,894 + MMJJ_RS04485 0.81 -4.5 834,894 + MMJJ_RS04485 0.81 -4.6 834,894 + MMJJ_RS04485 0.81 -4.6 834,904 - MMJJ_RS04485 0.83 -2.7
Or see this region's nucleotide sequence