Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04005 and MMJJ_RS04010 are separated by 532 nucleotides MMJJ_RS04010 and MMJJ_RS04015 are separated by 98 nucleotides MMJJ_RS04015 and MMJJ_RS04020 are separated by 29 nucleotides
MMJJ_RS04005: MMJJ_RS04005 - acetate--CoA ligase family protein, at 746,108 to 748,216
_RS04005
MMJJ_RS04010: MMJJ_RS04010 - archaeal proteasome endopeptidase complex subunit alpha, at 748,749 to 749,528
_RS04010
MMJJ_RS04015: MMJJ_RS04015 - ribosome assembly factor SBDS, at 749,627 to 750,331
_RS04015
MMJJ_RS04020: MMJJ_RS04020 - 50S ribosomal protein L37Ae, at 750,361 to 750,651
_RS04020
Position (kb) 748
749
750 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 747.878 kb on + strand, within MMJJ_RS04005 at 747.889 kb on - strand, within MMJJ_RS04005 at 747.940 kb on + strand, within MMJJ_RS04005 at 747.954 kb on - strand, within MMJJ_RS04005 at 748.008 kb on - strand at 748.008 kb on - strand at 748.028 kb on - strand at 748.031 kb on + strand at 748.031 kb on + strand at 748.039 kb on + strand at 748.080 kb on - strand at 748.121 kb on - strand at 748.160 kb on + strand at 748.204 kb on - strand at 748.205 kb on + strand at 748.205 kb on + strand at 748.215 kb on + strand at 748.218 kb on + strand at 748.239 kb on - strand at 748.244 kb on - strand at 748.244 kb on - strand at 748.244 kb on - strand at 748.245 kb on + strand at 748.314 kb on + strand at 748.347 kb on - strand at 748.349 kb on - strand at 748.349 kb on - strand at 748.350 kb on + strand at 748.350 kb on + strand at 748.350 kb on + strand at 748.350 kb on + strand at 748.350 kb on + strand at 748.351 kb on - strand at 748.351 kb on - strand at 748.351 kb on - strand at 748.352 kb on + strand at 748.352 kb on + strand at 748.364 kb on + strand at 748.364 kb on + strand at 748.575 kb on - strand at 748.576 kb on + strand at 748.578 kb on - strand at 748.578 kb on - strand at 748.579 kb on + strand at 748.579 kb on + strand at 748.585 kb on - strand at 748.585 kb on - strand at 748.585 kb on - strand at 748.586 kb on + strand at 748.586 kb on + strand at 748.591 kb on - strand at 748.591 kb on - strand at 748.599 kb on - strand at 748.599 kb on - strand at 748.600 kb on + strand at 748.600 kb on + strand at 748.601 kb on - strand at 748.601 kb on - strand at 748.601 kb on - strand at 748.601 kb on - strand at 748.602 kb on + strand at 748.614 kb on + strand at 748.617 kb on + strand at 748.618 kb on - strand at 748.619 kb on + strand at 748.619 kb on + strand at 748.621 kb on - strand at 748.621 kb on - strand at 748.621 kb on - strand at 748.624 kb on - strand at 748.624 kb on - strand at 748.624 kb on - strand at 748.624 kb on - strand at 748.625 kb on + strand at 748.625 kb on + strand at 748.625 kb on + strand at 748.626 kb on - strand at 748.626 kb on - strand at 748.626 kb on - strand at 748.627 kb on + strand at 748.627 kb on + strand at 748.627 kb on + strand at 748.627 kb on + strand at 748.628 kb on - strand at 748.628 kb on - strand at 748.651 kb on - strand at 748.660 kb on + strand at 748.660 kb on + strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.663 kb on - strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.664 kb on + strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.665 kb on - strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.666 kb on + strand at 748.667 kb on - strand at 748.668 kb on + strand at 748.669 kb on - strand at 748.691 kb on - strand at 748.691 kb on - strand at 748.694 kb on - strand at 748.694 kb on - strand at 748.695 kb on + strand at 748.695 kb on + strand at 748.695 kb on + strand at 748.722 kb on + strand at 748.725 kb on + strand at 748.746 kb on - strand at 748.746 kb on - strand at 748.746 kb on - strand at 748.746 kb on - strand at 748.747 kb on + strand at 748.747 kb on + strand at 748.747 kb on + strand at 748.748 kb on - strand at 748.748 kb on - strand at 748.749 kb on + strand at 748.795 kb on - strand at 748.830 kb on - strand, within MMJJ_RS04010 at 748.830 kb on - strand, within MMJJ_RS04010 at 748.859 kb on - strand, within MMJJ_RS04010 at 748.860 kb on + strand, within MMJJ_RS04010 at 748.860 kb on + strand, within MMJJ_RS04010 at 748.867 kb on - strand, within MMJJ_RS04010 at 748.874 kb on - strand, within MMJJ_RS04010 at 748.916 kb on - strand, within MMJJ_RS04010 at 748.928 kb on + strand, within MMJJ_RS04010 at 748.985 kb on - strand, within MMJJ_RS04010 at 749.061 kb on - strand, within MMJJ_RS04010 at 749.121 kb on - strand, within MMJJ_RS04010 at 749.121 kb on - strand, within MMJJ_RS04010 at 749.121 kb on - strand, within MMJJ_RS04010 at 749.122 kb on + strand, within MMJJ_RS04010 at 749.122 kb on + strand, within MMJJ_RS04010 at 749.122 kb on + strand, within MMJJ_RS04010 at 749.122 kb on + strand, within MMJJ_RS04010 at 749.168 kb on - strand, within MMJJ_RS04010 at 749.224 kb on - strand, within MMJJ_RS04010 at 749.228 kb on + strand, within MMJJ_RS04010 at 749.240 kb on - strand, within MMJJ_RS04010 at 749.583 kb on - strand at 750.323 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 747,878 + MMJJ_RS04005 0.84 -0.5 747,889 - MMJJ_RS04005 0.84 -0.3 747,940 + MMJJ_RS04005 0.87 +0.8 747,954 - MMJJ_RS04005 0.88 -1.8 748,008 - -1.2 748,008 - -1.5 748,028 - -0.1 748,031 + +1.0 748,031 + +0.6 748,039 + +0.6 748,080 - -1.3 748,121 - +1.2 748,160 + -1.2 748,204 - +0.9 748,205 + +0.3 748,205 + -1.5 748,215 + +0.7 748,218 + -1.0 748,239 - -0.4 748,244 - -0.8 748,244 - -0.3 748,244 - -0.8 748,245 + -1.6 748,314 + -0.5 748,347 - +0.1 748,349 - +1.1 748,349 - +0.6 748,350 + -1.5 748,350 + +1.1 748,350 + -1.3 748,350 + -0.9 748,350 + +0.8 748,351 - +0.1 748,351 - +0.7 748,351 - +0.9 748,352 + +1.1 748,352 + -1.0 748,364 + -1.8 748,364 + +0.2 748,575 - -2.1 748,576 + +0.7 748,578 - -0.7 748,578 - +0.4 748,579 + +0.1 748,579 + -0.6 748,585 - +0.7 748,585 - +0.2 748,585 - +0.4 748,586 + -0.7 748,586 + -0.2 748,591 - -0.8 748,591 - +0.9 748,599 - +0.1 748,599 - -1.4 748,600 + +2.2 748,600 + +0.8 748,601 - -1.1 748,601 - +0.2 748,601 - +0.1 748,601 - -0.3 748,602 + -1.3 748,614 + +1.2 748,617 + -1.1 748,618 - -0.0 748,619 + -0.6 748,619 + +0.1 748,621 - -1.5 748,621 - -0.2 748,621 - +0.4 748,624 - +1.4 748,624 - +0.2 748,624 - -0.9 748,624 - -0.9 748,625 + -0.8 748,625 + -1.8 748,625 + +1.0 748,626 - -0.5 748,626 - +0.3 748,626 - -0.3 748,627 + +1.4 748,627 + +0.2 748,627 + +0.7 748,627 + -0.6 748,628 - -0.6 748,628 - -0.6 748,651 - -0.5 748,660 + +1.2 748,660 + +0.3 748,663 - -1.4 748,663 - -1.3 748,663 - -2.7 748,663 - -1.0 748,663 - -0.5 748,663 - -1.0 748,663 - -0.8 748,663 - -0.5 748,663 - -2.4 748,663 - -1.4 748,663 - -0.6 748,664 + +1.6 748,664 + -0.3 748,664 + -1.9 748,664 + -0.7 748,664 + -1.2 748,664 + -1.2 748,664 + -0.8 748,665 - -3.4 748,665 - -2.2 748,665 - -0.8 748,665 - -1.7 748,665 - -2.9 748,665 - -0.6 748,665 - -1.7 748,665 - -1.8 748,665 - -2.1 748,665 - -0.8 748,665 - -2.5 748,665 - -1.6 748,665 - -0.2 748,665 - +0.3 748,665 - -0.6 748,665 - -2.1 748,666 + -1.5 748,666 + -1.8 748,666 + -1.3 748,666 + -2.5 748,666 + -2.7 748,666 + -1.5 748,666 + -2.8 748,666 + -2.0 748,666 + -1.5 748,667 - -1.5 748,668 + -2.6 748,669 - -1.7 748,691 - -3.8 748,691 - -3.2 748,694 - -1.3 748,694 - -1.8 748,695 + -2.0 748,695 + -0.0 748,695 + -3.2 748,722 + -2.2 748,725 + -2.4 748,746 - -4.4 748,746 - -3.0 748,746 - +0.9 748,746 - -2.1 748,747 + -4.2 748,747 + -4.2 748,747 + -3.5 748,748 - -0.5 748,748 - -2.6 748,749 + -3.2 748,795 - -4.8 748,830 - MMJJ_RS04010 0.10 -2.5 748,830 - MMJJ_RS04010 0.10 -4.6 748,859 - MMJJ_RS04010 0.14 -3.7 748,860 + MMJJ_RS04010 0.14 -0.0 748,860 + MMJJ_RS04010 0.14 -3.9 748,867 - MMJJ_RS04010 0.15 -2.3 748,874 - MMJJ_RS04010 0.16 -3.4 748,916 - MMJJ_RS04010 0.21 -4.2 748,928 + MMJJ_RS04010 0.23 -1.9 748,985 - MMJJ_RS04010 0.30 -3.1 749,061 - MMJJ_RS04010 0.40 -3.4 749,121 - MMJJ_RS04010 0.48 -1.2 749,121 - MMJJ_RS04010 0.48 -3.4 749,121 - MMJJ_RS04010 0.48 -5.0 749,122 + MMJJ_RS04010 0.48 -2.8 749,122 + MMJJ_RS04010 0.48 -2.4 749,122 + MMJJ_RS04010 0.48 -3.3 749,122 + MMJJ_RS04010 0.48 -2.7 749,168 - MMJJ_RS04010 0.54 -1.1 749,224 - MMJJ_RS04010 0.61 -3.2 749,228 + MMJJ_RS04010 0.61 -1.5 749,240 - MMJJ_RS04010 0.63 -2.7 749,583 - +0.9 750,323 - -1.0
Or see this region's nucleotide sequence