Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03840 and MMJJ_RS03845 are separated by 134 nucleotides MMJJ_RS03845 and MMJJ_RS03850 are separated by 159 nucleotides MMJJ_RS03850 and MMJJ_RS03855 are separated by 199 nucleotides
MMJJ_RS03840: MMJJ_RS03840 - DHH family phosphoesterase, at 713,710 to 715,170
_RS03840
MMJJ_RS03845: MMJJ_RS03845 - translation initiation factor IF-2, at 715,305 to 717,101
_RS03845
MMJJ_RS03850: MMJJ_RS03850 - nucleoside-diphosphate kinase, at 717,261 to 717,680
_RS03850
MMJJ_RS03855: MMJJ_RS03855 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 717,880 to 718,356
_RS03855
Position (kb)
715
716
717
718 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 714.517 kb on - strand, within MMJJ_RS03840 at 714.517 kb on - strand, within MMJJ_RS03840 at 714.545 kb on - strand, within MMJJ_RS03840 at 714.546 kb on + strand, within MMJJ_RS03840 at 714.658 kb on - strand, within MMJJ_RS03840 at 714.662 kb on + strand, within MMJJ_RS03840 at 714.710 kb on - strand, within MMJJ_RS03840 at 714.832 kb on - strand, within MMJJ_RS03840 at 714.833 kb on + strand, within MMJJ_RS03840 at 714.834 kb on - strand at 714.840 kb on - strand at 714.844 kb on - strand, within MMJJ_RS03840 at 714.881 kb on + strand at 714.897 kb on - strand at 714.899 kb on - strand, within MMJJ_RS03840 at 715.036 kb on - strand at 715.040 kb on - strand at 715.041 kb on + strand at 715.091 kb on + strand at 715.157 kb on + strand at 715.202 kb on - strand at 715.202 kb on - strand at 715.229 kb on - strand at 715.260 kb on - strand at 715.260 kb on - strand at 715.261 kb on + strand at 715.261 kb on + strand at 715.262 kb on - strand at 715.262 kb on - strand at 715.262 kb on - strand at 715.263 kb on + strand at 715.263 kb on + strand at 715.263 kb on + strand at 715.306 kb on - strand at 715.307 kb on + strand at 715.354 kb on - strand at 715.355 kb on + strand at 715.449 kb on - strand at 715.485 kb on + strand, within MMJJ_RS03845 at 715.784 kb on - strand, within MMJJ_RS03845 at 715.812 kb on + strand, within MMJJ_RS03845 at 716.015 kb on - strand, within MMJJ_RS03845 at 716.016 kb on + strand, within MMJJ_RS03845 at 716.016 kb on + strand, within MMJJ_RS03845 at 716.141 kb on - strand, within MMJJ_RS03845 at 716.141 kb on - strand, within MMJJ_RS03845 at 716.166 kb on - strand, within MMJJ_RS03845 at 716.167 kb on + strand, within MMJJ_RS03845 at 716.167 kb on + strand, within MMJJ_RS03845 at 716.712 kb on + strand, within MMJJ_RS03845 at 716.754 kb on + strand at 717.036 kb on - strand at 717.115 kb on - strand at 717.121 kb on - strand at 717.158 kb on + strand at 717.159 kb on - strand at 717.159 kb on - strand at 717.196 kb on - strand at 717.204 kb on + strand at 717.207 kb on - strand at 717.208 kb on + strand at 717.208 kb on + strand at 717.208 kb on + strand at 717.212 kb on - strand at 717.231 kb on + strand at 717.255 kb on - strand at 717.282 kb on - strand at 717.303 kb on - strand, within MMJJ_RS03850 at 717.304 kb on + strand, within MMJJ_RS03850 at 717.324 kb on - strand, within MMJJ_RS03850 at 717.324 kb on - strand, within MMJJ_RS03850 at 717.326 kb on - strand, within MMJJ_RS03850 at 717.340 kb on - strand, within MMJJ_RS03850 at 717.340 kb on - strand, within MMJJ_RS03850 at 717.341 kb on + strand, within MMJJ_RS03850 at 717.342 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.392 kb on - strand, within MMJJ_RS03850 at 717.393 kb on + strand, within MMJJ_RS03850 at 717.393 kb on + strand, within MMJJ_RS03850 at 717.483 kb on - strand, within MMJJ_RS03850 at 717.520 kb on - strand, within MMJJ_RS03850 at 717.681 kb on + strand at 717.683 kb on + strand at 717.704 kb on + strand at 717.704 kb on + strand at 717.728 kb on - strand at 717.729 kb on + strand at 717.732 kb on + strand at 717.735 kb on - strand at 717.736 kb on + strand at 717.746 kb on - strand at 717.746 kb on - strand at 717.750 kb on - strand at 717.751 kb on + strand at 717.751 kb on + strand at 717.756 kb on - strand at 717.756 kb on - strand at 717.756 kb on - strand at 717.757 kb on + strand at 717.759 kb on + strand at 717.759 kb on + strand at 717.761 kb on - strand at 717.761 kb on - strand at 717.761 kb on - strand at 717.762 kb on + strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.765 kb on - strand at 717.766 kb on + strand at 717.766 kb on + strand at 717.766 kb on + strand at 717.771 kb on - strand at 717.771 kb on - strand at 717.791 kb on - strand at 717.791 kb on - strand at 717.798 kb on + strand at 717.798 kb on + strand at 717.798 kb on + strand at 717.806 kb on + strand at 717.824 kb on + strand at 717.824 kb on + strand at 717.824 kb on + strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.827 kb on - strand at 717.828 kb on + strand at 717.828 kb on + strand at 717.852 kb on + strand at 717.860 kb on + strand at 717.971 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 714,517 - MMJJ_RS03840 0.55 -2.8 714,517 - MMJJ_RS03840 0.55 -2.3 714,545 - MMJJ_RS03840 0.57 -2.1 714,546 + MMJJ_RS03840 0.57 -2.8 714,658 - MMJJ_RS03840 0.65 -2.0 714,662 + MMJJ_RS03840 0.65 -2.4 714,710 - MMJJ_RS03840 0.68 -0.5 714,832 - MMJJ_RS03840 0.77 -1.3 714,833 + MMJJ_RS03840 0.77 -2.1 714,834 - -1.3 714,840 - +0.0 714,844 - MMJJ_RS03840 0.78 -1.3 714,881 + -1.5 714,897 - -1.3 714,899 - MMJJ_RS03840 0.81 -2.5 715,036 - -0.3 715,040 - -2.3 715,041 + -1.4 715,091 + -1.0 715,157 + -3.2 715,202 - -2.2 715,202 - -2.4 715,229 - -3.7 715,260 - -3.1 715,260 - -2.1 715,261 + -1.3 715,261 + -1.0 715,262 - +0.3 715,262 - -1.3 715,262 - +1.0 715,263 + +0.2 715,263 + -0.5 715,263 + -0.2 715,306 - -0.1 715,307 + +0.4 715,354 - -1.6 715,355 + -3.9 715,449 - -2.3 715,485 + MMJJ_RS03845 0.10 -1.5 715,784 - MMJJ_RS03845 0.27 -1.5 715,812 + MMJJ_RS03845 0.28 -2.4 716,015 - MMJJ_RS03845 0.40 -2.4 716,016 + MMJJ_RS03845 0.40 -3.0 716,016 + MMJJ_RS03845 0.40 -1.3 716,141 - MMJJ_RS03845 0.47 -1.0 716,141 - MMJJ_RS03845 0.47 -1.5 716,166 - MMJJ_RS03845 0.48 -3.0 716,167 + MMJJ_RS03845 0.48 +0.0 716,167 + MMJJ_RS03845 0.48 -2.0 716,712 + MMJJ_RS03845 0.78 -2.3 716,754 + +1.8 717,036 - -1.9 717,115 - -2.4 717,121 - -0.5 717,158 + -2.4 717,159 - -0.7 717,159 - -2.7 717,196 - -1.3 717,204 + -0.5 717,207 - -1.7 717,208 + -3.5 717,208 + -3.0 717,208 + -2.7 717,212 - -1.1 717,231 + -0.3 717,255 - -1.3 717,282 - -0.3 717,303 - MMJJ_RS03850 0.10 -1.1 717,304 + MMJJ_RS03850 0.10 +1.2 717,324 - MMJJ_RS03850 0.15 +2.0 717,324 - MMJJ_RS03850 0.15 -2.5 717,326 - MMJJ_RS03850 0.15 +0.4 717,340 - MMJJ_RS03850 0.19 -0.3 717,340 - MMJJ_RS03850 0.19 +0.9 717,341 + MMJJ_RS03850 0.19 -1.1 717,342 - MMJJ_RS03850 0.19 +1.0 717,392 - MMJJ_RS03850 0.31 +0.3 717,392 - MMJJ_RS03850 0.31 +0.1 717,392 - MMJJ_RS03850 0.31 -0.8 717,393 + MMJJ_RS03850 0.31 +0.9 717,393 + MMJJ_RS03850 0.31 -0.6 717,483 - MMJJ_RS03850 0.53 +0.5 717,520 - MMJJ_RS03850 0.62 -0.8 717,681 + +0.7 717,683 + -1.2 717,704 + +1.6 717,704 + +0.5 717,728 - -1.1 717,729 + +1.4 717,732 + +0.9 717,735 - +0.4 717,736 + +0.8 717,746 - -0.1 717,746 - +0.4 717,750 - +1.0 717,751 + -1.3 717,751 + +0.7 717,756 - -1.1 717,756 - -1.2 717,756 - -0.1 717,757 + -0.5 717,759 + -1.5 717,759 + -0.3 717,761 - -0.6 717,761 - +1.2 717,761 - +0.4 717,762 + +0.7 717,765 - -1.2 717,765 - +0.0 717,765 - -1.3 717,765 - +0.6 717,766 + +1.9 717,766 + -0.6 717,766 + +1.7 717,771 - -0.4 717,771 - +1.5 717,791 - -0.7 717,791 - -0.2 717,798 + +1.0 717,798 + -0.8 717,798 + +0.7 717,806 + -1.1 717,824 + +0.0 717,824 + -0.2 717,824 + +0.0 717,827 - -1.4 717,827 - -2.9 717,827 - -1.5 717,827 - +0.7 717,827 - +1.4 717,827 - +0.0 717,828 + +0.9 717,828 + +0.0 717,852 + +0.0 717,860 + -2.4 717,971 - -1.0
Or see this region's nucleotide sequence