Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03810 and MMJJ_RS03815 are separated by 72 nucleotides MMJJ_RS03815 and MMJJ_RS03820 are separated by 245 nucleotides MMJJ_RS03820 and MMJJ_RS03825 are separated by 25 nucleotides MMJJ_RS03825 and MMJJ_RS03830 are separated by 37 nucleotides
MMJJ_RS03810: MMJJ_RS03810 - AIR synthase-related protein, at 708,742 to 710,085
_RS03810
MMJJ_RS03815: MMJJ_RS03815 - nascent polypeptide-associated complex protein, at 710,158 to 710,538
_RS03815
MMJJ_RS03820: MMJJ_RS03820 - hydrogenase formation protein HypD, at 710,784 to 711,866
_RS03820
MMJJ_RS03825: MMJJ_RS03825 - DUF1624 domain-containing protein, at 711,892 to 712,632
_RS03825
MMJJ_RS03830: MMJJ_RS03830 - CBS domain-containing protein, at 712,670 to 713,464
_RS03830
Position (kb) 710
711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 709.885 kb on - strand, within MMJJ_RS03810 at 710.128 kb on + strand at 710.189 kb on + strand at 710.209 kb on - strand, within MMJJ_RS03815 at 710.210 kb on + strand, within MMJJ_RS03815 at 710.433 kb on - strand, within MMJJ_RS03815 at 710.434 kb on + strand, within MMJJ_RS03815 at 710.541 kb on - strand at 710.541 kb on - strand at 710.542 kb on + strand at 710.542 kb on + strand at 710.542 kb on + strand at 710.552 kb on - strand at 710.552 kb on - strand at 710.553 kb on + strand at 710.556 kb on - strand at 710.572 kb on + strand at 710.572 kb on + strand at 710.576 kb on - strand at 710.580 kb on - strand at 710.580 kb on - strand at 710.582 kb on - strand at 710.598 kb on - strand at 710.598 kb on - strand at 710.598 kb on - strand at 710.600 kb on - strand at 710.601 kb on + strand at 710.604 kb on - strand at 710.604 kb on - strand at 710.604 kb on - strand at 710.604 kb on - strand at 710.604 kb on - strand at 710.605 kb on + strand at 710.605 kb on + strand at 710.605 kb on + strand at 710.618 kb on + strand at 710.685 kb on - strand at 710.697 kb on + strand at 710.697 kb on + strand at 710.697 kb on + strand at 710.711 kb on + strand at 710.728 kb on - strand at 710.728 kb on - strand at 710.734 kb on - strand at 710.735 kb on + strand at 710.737 kb on + strand at 710.738 kb on - strand at 710.738 kb on - strand at 710.738 kb on - strand at 710.738 kb on - strand at 710.738 kb on - strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.739 kb on + strand at 710.741 kb on - strand at 710.741 kb on - strand at 710.741 kb on - strand at 710.742 kb on + strand at 710.742 kb on + strand at 710.742 kb on + strand at 710.751 kb on - strand at 710.761 kb on + strand at 710.765 kb on - strand at 710.765 kb on - strand at 710.766 kb on + strand at 710.783 kb on - strand at 710.784 kb on + strand at 710.784 kb on + strand at 710.991 kb on + strand, within MMJJ_RS03820 at 711.066 kb on - strand at 711.066 kb on - strand at 711.067 kb on + strand, within MMJJ_RS03820 at 711.067 kb on + strand, within MMJJ_RS03820 at 711.238 kb on - strand, within MMJJ_RS03820 at 711.241 kb on - strand, within MMJJ_RS03820 at 711.334 kb on - strand, within MMJJ_RS03820 at 711.387 kb on - strand, within MMJJ_RS03820 at 711.388 kb on + strand, within MMJJ_RS03820 at 711.388 kb on + strand at 711.390 kb on - strand, within MMJJ_RS03820 at 711.390 kb on - strand, within MMJJ_RS03820 at 711.391 kb on + strand at 711.476 kb on + strand, within MMJJ_RS03820 at 711.476 kb on + strand at 711.487 kb on - strand, within MMJJ_RS03820 at 711.849 kb on - strand at 711.854 kb on - strand at 711.861 kb on + strand at 711.864 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.868 kb on - strand at 711.869 kb on + strand at 711.869 kb on + strand at 711.873 kb on - strand at 711.894 kb on + strand at 711.914 kb on + strand at 711.926 kb on + strand at 711.963 kb on - strand at 711.965 kb on - strand at 711.966 kb on + strand, within MMJJ_RS03825 at 711.971 kb on + strand, within MMJJ_RS03825 at 711.978 kb on - strand, within MMJJ_RS03825 at 711.978 kb on - strand, within MMJJ_RS03825 at 711.978 kb on - strand, within MMJJ_RS03825 at 711.978 kb on - strand, within MMJJ_RS03825 at 711.997 kb on - strand, within MMJJ_RS03825 at 711.998 kb on + strand, within MMJJ_RS03825 at 711.998 kb on + strand, within MMJJ_RS03825 at 712.087 kb on - strand, within MMJJ_RS03825 at 712.087 kb on - strand, within MMJJ_RS03825 at 712.088 kb on + strand, within MMJJ_RS03825 at 712.088 kb on + strand, within MMJJ_RS03825 at 712.091 kb on - strand, within MMJJ_RS03825 at 712.091 kb on - strand, within MMJJ_RS03825 at 712.091 kb on - strand, within MMJJ_RS03825 at 712.091 kb on - strand, within MMJJ_RS03825 at 712.092 kb on + strand, within MMJJ_RS03825 at 712.092 kb on + strand, within MMJJ_RS03825 at 712.094 kb on + strand, within MMJJ_RS03825 at 712.121 kb on - strand, within MMJJ_RS03825 at 712.122 kb on + strand, within MMJJ_RS03825 at 712.151 kb on + strand, within MMJJ_RS03825 at 712.166 kb on + strand, within MMJJ_RS03825 at 712.166 kb on + strand, within MMJJ_RS03825 at 712.172 kb on - strand, within MMJJ_RS03825 at 712.173 kb on + strand, within MMJJ_RS03825 at 712.173 kb on + strand, within MMJJ_RS03825 at 712.173 kb on + strand, within MMJJ_RS03825 at 712.176 kb on - strand, within MMJJ_RS03825 at 712.180 kb on - strand, within MMJJ_RS03825 at 712.199 kb on + strand, within MMJJ_RS03825 at 712.207 kb on - strand, within MMJJ_RS03825 at 712.208 kb on + strand, within MMJJ_RS03825 at 712.208 kb on + strand, within MMJJ_RS03825 at 712.208 kb on + strand, within MMJJ_RS03825 at 712.261 kb on - strand, within MMJJ_RS03825 at 712.276 kb on + strand, within MMJJ_RS03825 at 712.311 kb on + strand, within MMJJ_RS03825 at 712.311 kb on + strand, within MMJJ_RS03825 at 712.312 kb on - strand, within MMJJ_RS03825 at 712.351 kb on - strand, within MMJJ_RS03825 at 712.352 kb on + strand, within MMJJ_RS03825 at 712.352 kb on + strand, within MMJJ_RS03825 at 712.353 kb on - strand, within MMJJ_RS03825 at 712.354 kb on + strand, within MMJJ_RS03825 at 712.367 kb on + strand, within MMJJ_RS03825 at 712.395 kb on + strand, within MMJJ_RS03825 at 712.395 kb on + strand, within MMJJ_RS03825 at 712.446 kb on - strand, within MMJJ_RS03825 at 712.464 kb on - strand, within MMJJ_RS03825 at 712.465 kb on + strand, within MMJJ_RS03825 at 712.465 kb on + strand, within MMJJ_RS03825 at 712.469 kb on + strand, within MMJJ_RS03825 at 712.518 kb on + strand, within MMJJ_RS03825 at 712.518 kb on + strand, within MMJJ_RS03825 at 712.544 kb on + strand, within MMJJ_RS03825 at 712.553 kb on + strand, within MMJJ_RS03825 at 712.567 kb on + strand at 712.567 kb on + strand at 712.576 kb on - strand at 712.576 kb on - strand at 712.577 kb on + strand at 712.577 kb on + strand at 712.579 kb on - strand at 712.650 kb on + strand at 712.658 kb on + strand at 712.684 kb on - strand at 712.699 kb on - strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.700 kb on + strand at 712.701 kb on - strand at 712.741 kb on - strand at 712.745 kb on + strand at 712.811 kb on - strand, within MMJJ_RS03830
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 709,885 - MMJJ_RS03810 0.85 -2.1 710,128 + +0.5 710,189 + +2.4 710,209 - MMJJ_RS03815 0.13 +0.0 710,210 + MMJJ_RS03815 0.14 -1.1 710,433 - MMJJ_RS03815 0.72 -3.3 710,434 + MMJJ_RS03815 0.72 -1.7 710,541 - +0.9 710,541 - -1.0 710,542 + -1.1 710,542 + -1.0 710,542 + +0.2 710,552 - +0.5 710,552 - -1.6 710,553 + +0.9 710,556 - -0.3 710,572 + -0.6 710,572 + -1.0 710,576 - +0.2 710,580 - +0.4 710,580 - -1.2 710,582 - -1.9 710,598 - +0.4 710,598 - +1.5 710,598 - +0.1 710,600 - +0.5 710,601 + +0.9 710,604 - -0.7 710,604 - +0.8 710,604 - +0.3 710,604 - +1.3 710,604 - -1.1 710,605 + +1.2 710,605 + -2.0 710,605 + +1.6 710,618 + +0.9 710,685 - -1.7 710,697 + +0.8 710,697 + -2.9 710,697 + -0.4 710,711 + -1.2 710,728 - -0.0 710,728 - -0.4 710,734 - +0.9 710,735 + -0.1 710,737 + -0.4 710,738 - +1.6 710,738 - +0.5 710,738 - +0.6 710,738 - -1.5 710,738 - +1.8 710,739 + +1.7 710,739 + +0.2 710,739 + -0.4 710,739 + +1.1 710,739 + +0.3 710,739 + -1.7 710,739 + -0.4 710,741 - -1.2 710,741 - +1.4 710,741 - +1.0 710,742 + -0.9 710,742 + +1.1 710,742 + -0.8 710,751 - -0.2 710,761 + -2.2 710,765 - -2.3 710,765 - -0.4 710,766 + -0.7 710,783 - -2.5 710,784 + -2.9 710,784 + -0.5 710,991 + MMJJ_RS03820 0.19 -2.3 711,066 - -1.0 711,066 - -1.5 711,067 + MMJJ_RS03820 0.26 -3.1 711,067 + MMJJ_RS03820 0.26 -1.5 711,238 - MMJJ_RS03820 0.42 -2.4 711,241 - MMJJ_RS03820 0.42 -1.8 711,334 - MMJJ_RS03820 0.51 -2.8 711,387 - MMJJ_RS03820 0.56 -1.5 711,388 + MMJJ_RS03820 0.56 -1.9 711,388 + -1.3 711,390 - MMJJ_RS03820 0.56 -0.7 711,390 - MMJJ_RS03820 0.56 -1.4 711,391 + +0.0 711,476 + MMJJ_RS03820 0.64 -1.1 711,476 + +0.0 711,487 - MMJJ_RS03820 0.65 -2.3 711,849 - -0.9 711,854 - +0.3 711,861 + -0.1 711,864 - +0.5 711,868 - +0.8 711,868 - +0.1 711,868 - +1.0 711,868 - +0.7 711,868 - -0.9 711,868 - -2.0 711,868 - -1.5 711,868 - -0.7 711,868 - -1.0 711,868 - -0.2 711,869 + +1.1 711,869 + +0.4 711,873 - -1.3 711,894 + +1.1 711,914 + -0.4 711,926 + +0.8 711,963 - +0.0 711,965 - -1.5 711,966 + MMJJ_RS03825 0.10 +0.3 711,971 + MMJJ_RS03825 0.11 +0.5 711,978 - MMJJ_RS03825 0.12 -0.3 711,978 - MMJJ_RS03825 0.12 -0.3 711,978 - MMJJ_RS03825 0.12 +0.2 711,978 - MMJJ_RS03825 0.12 +0.4 711,997 - MMJJ_RS03825 0.14 -0.6 711,998 + MMJJ_RS03825 0.14 -0.5 711,998 + MMJJ_RS03825 0.14 +0.2 712,087 - MMJJ_RS03825 0.26 -0.1 712,087 - MMJJ_RS03825 0.26 +0.7 712,088 + MMJJ_RS03825 0.26 +0.0 712,088 + MMJJ_RS03825 0.26 -1.0 712,091 - MMJJ_RS03825 0.27 +0.0 712,091 - MMJJ_RS03825 0.27 +0.3 712,091 - MMJJ_RS03825 0.27 +0.8 712,091 - MMJJ_RS03825 0.27 +1.1 712,092 + MMJJ_RS03825 0.27 -0.1 712,092 + MMJJ_RS03825 0.27 -1.0 712,094 + MMJJ_RS03825 0.27 +1.2 712,121 - MMJJ_RS03825 0.31 +0.5 712,122 + MMJJ_RS03825 0.31 +0.8 712,151 + MMJJ_RS03825 0.35 -0.7 712,166 + MMJJ_RS03825 0.37 +1.7 712,166 + MMJJ_RS03825 0.37 +0.0 712,172 - MMJJ_RS03825 0.38 -1.0 712,173 + MMJJ_RS03825 0.38 +0.2 712,173 + MMJJ_RS03825 0.38 -0.4 712,173 + MMJJ_RS03825 0.38 -2.8 712,176 - MMJJ_RS03825 0.38 +0.0 712,180 - MMJJ_RS03825 0.39 -0.3 712,199 + MMJJ_RS03825 0.41 -0.6 712,207 - MMJJ_RS03825 0.43 -1.5 712,208 + MMJJ_RS03825 0.43 -0.7 712,208 + MMJJ_RS03825 0.43 -2.0 712,208 + MMJJ_RS03825 0.43 -1.3 712,261 - MMJJ_RS03825 0.50 -0.1 712,276 + MMJJ_RS03825 0.52 +0.9 712,311 + MMJJ_RS03825 0.57 +1.5 712,311 + MMJJ_RS03825 0.57 -0.4 712,312 - MMJJ_RS03825 0.57 -0.3 712,351 - MMJJ_RS03825 0.62 -0.8 712,352 + MMJJ_RS03825 0.62 +0.1 712,352 + MMJJ_RS03825 0.62 -0.4 712,353 - MMJJ_RS03825 0.62 -0.8 712,354 + MMJJ_RS03825 0.62 -0.8 712,367 + MMJJ_RS03825 0.64 -0.3 712,395 + MMJJ_RS03825 0.68 +0.9 712,395 + MMJJ_RS03825 0.68 -1.8 712,446 - MMJJ_RS03825 0.75 +0.5 712,464 - MMJJ_RS03825 0.77 +0.3 712,465 + MMJJ_RS03825 0.77 -0.2 712,465 + MMJJ_RS03825 0.77 -1.0 712,469 + MMJJ_RS03825 0.78 +0.2 712,518 + MMJJ_RS03825 0.84 -0.1 712,518 + MMJJ_RS03825 0.84 +0.9 712,544 + MMJJ_RS03825 0.88 -1.1 712,553 + MMJJ_RS03825 0.89 +0.9 712,567 + -1.0 712,567 + -0.5 712,576 - +0.4 712,576 - +0.6 712,577 + -2.0 712,577 + -0.2 712,579 - -0.7 712,650 + -2.0 712,658 + -1.1 712,684 - -0.3 712,699 - +0.5 712,700 + -1.2 712,700 + +0.1 712,700 + -0.2 712,700 + -3.0 712,700 + -0.6 712,701 - -1.1 712,741 - -0.3 712,745 + -1.1 712,811 - MMJJ_RS03830 0.18 -2.3
Or see this region's nucleotide sequence