Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03775 and MMJJ_RS03780 are separated by 226 nucleotides MMJJ_RS03780 and MMJJ_RS03785 are separated by 57 nucleotides MMJJ_RS03785 and MMJJ_RS03790 are separated by 37 nucleotides
MMJJ_RS03775: MMJJ_RS03775 - 50S ribosomal protein L15e, at 703,055 to 703,639
_RS03775
MMJJ_RS03780: MMJJ_RS03780 - translation initiation factor IF-2 subunit beta, at 703,866 to 704,282
_RS03780
MMJJ_RS03785: MMJJ_RS03785 - hypothetical protein, at 704,340 to 705,428
_RS03785
MMJJ_RS03790: MMJJ_RS03790 - homoserine kinase, at 705,466 to 706,371
_RS03790
Position (kb) 704
705
706 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 703.624 kb on + strand at 703.781 kb on - strand at 703.782 kb on + strand at 703.782 kb on + strand at 703.783 kb on - strand at 703.783 kb on - strand at 704.279 kb on + strand at 704.280 kb on - strand at 704.281 kb on + strand at 704.281 kb on + strand at 704.281 kb on + strand at 704.281 kb on + strand at 704.284 kb on - strand at 704.309 kb on + strand at 704.309 kb on + strand at 704.313 kb on + strand at 704.724 kb on + strand, within MMJJ_RS03785 at 704.779 kb on + strand, within MMJJ_RS03785 at 704.782 kb on + strand, within MMJJ_RS03785 at 704.965 kb on - strand, within MMJJ_RS03785 at 704.973 kb on + strand, within MMJJ_RS03785 at 705.169 kb on + strand, within MMJJ_RS03785 at 705.275 kb on - strand, within MMJJ_RS03785 at 705.339 kb on + strand at 705.339 kb on + strand at 705.413 kb on + strand at 705.421 kb on - strand at 705.422 kb on + strand at 705.426 kb on - strand at 705.431 kb on + strand at 705.467 kb on - strand at 705.728 kb on + strand, within MMJJ_RS03790 at 706.242 kb on - strand at 706.355 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 703,624 + -2.1 703,781 - -1.8 703,782 + -1.0 703,782 + -1.0 703,783 - -2.1 703,783 - -2.5 704,279 + -1.3 704,280 - -2.8 704,281 + -0.3 704,281 + +0.0 704,281 + -0.8 704,281 + -1.0 704,284 - -2.4 704,309 + +1.1 704,309 + -0.5 704,313 + +1.3 704,724 + MMJJ_RS03785 0.35 -1.5 704,779 + MMJJ_RS03785 0.40 -2.5 704,782 + MMJJ_RS03785 0.41 -1.5 704,965 - MMJJ_RS03785 0.57 -1.5 704,973 + MMJJ_RS03785 0.58 -2.0 705,169 + MMJJ_RS03785 0.76 -1.0 705,275 - MMJJ_RS03785 0.86 -3.5 705,339 + -3.1 705,339 + -1.5 705,413 + -0.5 705,421 - -0.3 705,422 + -1.1 705,426 - +0.5 705,431 + +0.7 705,467 - +1.6 705,728 + MMJJ_RS03790 0.29 -1.8 706,242 - -1.5 706,355 + -2.0
Or see this region's nucleotide sequence