Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03745 and MMJJ_RS03750 are separated by 65 nucleotides MMJJ_RS03750 and MMJJ_RS03755 are separated by 20 nucleotides MMJJ_RS03755 and MMJJ_RS03760 are separated by 198 nucleotides MMJJ_RS03760 and MMJJ_RS03765 overlap by 1 nucleotides MMJJ_RS03765 and MMJJ_RS03770 are separated by 259 nucleotides
MMJJ_RS03745: MMJJ_RS03745 - N-glycosylase/DNA lyase, at 699,202 to 699,960
_RS03745
MMJJ_RS03750: MMJJ_RS03750 - rubredoxin, at 700,026 to 700,163
_RS03750
MMJJ_RS03755: MMJJ_RS03755 - hypothetical protein, at 700,184 to 700,543
_RS03755
MMJJ_RS03760: MMJJ_RS03760 - hydrogenase maturation nickel metallochaperone HypA, at 700,742 to 701,122
_RS03760
MMJJ_RS03765: MMJJ_RS03765 - hypothetical protein, at 701,122 to 701,583
_RS03765
MMJJ_RS03770: MMJJ_RS03770 - hypothetical protein, at 701,843 to 702,091
_RS03770
Position (kb) 700
701
702 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 699.773 kb on + strand, within MMJJ_RS03745 at 699.773 kb on + strand, within MMJJ_RS03745 at 699.849 kb on + strand, within MMJJ_RS03745 at 699.854 kb on - strand, within MMJJ_RS03745 at 699.855 kb on + strand, within MMJJ_RS03745 at 699.866 kb on - strand, within MMJJ_RS03745 at 699.935 kb on + strand at 699.938 kb on - strand at 699.939 kb on + strand at 699.939 kb on + strand at 699.942 kb on - strand at 699.956 kb on - strand at 699.957 kb on + strand at 699.964 kb on + strand at 699.977 kb on - strand at 699.979 kb on - strand at 699.980 kb on + strand at 700.004 kb on - strand at 700.019 kb on + strand at 700.025 kb on - strand at 700.026 kb on + strand at 700.049 kb on + strand, within MMJJ_RS03750 at 700.057 kb on + strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.060 kb on - strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand, within MMJJ_RS03750 at 700.061 kb on + strand at 700.061 kb on + strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.062 kb on - strand, within MMJJ_RS03750 at 700.063 kb on + strand, within MMJJ_RS03750 at 700.162 kb on + strand at 700.192 kb on + strand at 700.192 kb on - strand at 700.209 kb on - strand at 700.209 kb on - strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.210 kb on + strand at 700.214 kb on - strand at 700.215 kb on + strand at 700.218 kb on - strand at 700.219 kb on + strand at 700.219 kb on + strand at 700.256 kb on - strand, within MMJJ_RS03755 at 700.281 kb on - strand, within MMJJ_RS03755 at 700.281 kb on - strand, within MMJJ_RS03755 at 700.282 kb on + strand, within MMJJ_RS03755 at 700.282 kb on + strand, within MMJJ_RS03755 at 700.446 kb on + strand, within MMJJ_RS03755 at 700.446 kb on + strand, within MMJJ_RS03755 at 700.499 kb on - strand, within MMJJ_RS03755 at 700.523 kb on - strand at 700.589 kb on + strand at 700.596 kb on - strand at 700.598 kb on - strand at 700.598 kb on - strand at 700.598 kb on - strand at 700.599 kb on + strand at 700.600 kb on - strand at 700.601 kb on + strand at 700.601 kb on + strand at 700.601 kb on + strand at 700.604 kb on + strand at 700.635 kb on - strand at 700.636 kb on + strand at 700.681 kb on - strand at 700.685 kb on - strand at 700.685 kb on - strand at 700.685 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.695 kb on - strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.696 kb on + strand at 700.697 kb on - strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.698 kb on + strand at 700.712 kb on + strand at 700.727 kb on + strand at 700.727 kb on + strand at 700.730 kb on - strand at 700.731 kb on + strand at 700.778 kb on - strand at 700.808 kb on + strand, within MMJJ_RS03760 at 700.835 kb on - strand at 700.835 kb on - strand, within MMJJ_RS03760 at 700.863 kb on + strand, within MMJJ_RS03760 at 700.942 kb on - strand, within MMJJ_RS03760 at 700.946 kb on + strand, within MMJJ_RS03760 at 700.991 kb on - strand, within MMJJ_RS03760 at 701.021 kb on - strand, within MMJJ_RS03760 at 701.022 kb on + strand, within MMJJ_RS03760 at 701.023 kb on - strand, within MMJJ_RS03760 at 701.031 kb on + strand at 701.261 kb on - strand, within MMJJ_RS03765 at 701.261 kb on - strand, within MMJJ_RS03765 at 701.261 kb on - strand, within MMJJ_RS03765 at 701.262 kb on + strand, within MMJJ_RS03765 at 701.262 kb on + strand, within MMJJ_RS03765 at 701.285 kb on + strand, within MMJJ_RS03765 at 701.285 kb on + strand, within MMJJ_RS03765 at 701.288 kb on - strand, within MMJJ_RS03765 at 701.288 kb on - strand, within MMJJ_RS03765 at 701.289 kb on + strand, within MMJJ_RS03765 at 701.339 kb on + strand, within MMJJ_RS03765 at 701.339 kb on + strand, within MMJJ_RS03765 at 701.370 kb on - strand, within MMJJ_RS03765 at 701.373 kb on - strand, within MMJJ_RS03765 at 701.374 kb on + strand, within MMJJ_RS03765 at 701.374 kb on + strand, within MMJJ_RS03765 at 701.375 kb on - strand, within MMJJ_RS03765 at 701.375 kb on - strand, within MMJJ_RS03765 at 701.376 kb on + strand, within MMJJ_RS03765 at 701.379 kb on - strand, within MMJJ_RS03765 at 701.574 kb on - strand at 701.600 kb on + strand at 701.602 kb on + strand at 701.602 kb on + strand at 701.602 kb on + strand at 701.602 kb on + strand at 701.602 kb on + strand at 701.606 kb on - strand at 701.637 kb on - strand at 701.637 kb on - strand at 701.637 kb on - strand at 701.638 kb on + strand at 701.638 kb on + strand at 701.638 kb on + strand at 701.639 kb on - strand at 701.640 kb on + strand at 701.640 kb on + strand at 701.641 kb on - strand at 701.650 kb on - strand at 701.651 kb on + strand at 701.652 kb on - strand at 701.655 kb on - strand at 701.664 kb on + strand at 701.711 kb on - strand at 701.711 kb on - strand at 701.712 kb on + strand at 701.715 kb on - strand at 701.715 kb on - strand at 701.716 kb on + strand at 701.775 kb on + strand at 701.784 kb on - strand at 701.814 kb on + strand at 701.839 kb on - strand at 701.844 kb on - strand at 701.866 kb on - strand at 701.867 kb on + strand at 701.874 kb on - strand, within MMJJ_RS03770 at 701.874 kb on - strand, within MMJJ_RS03770 at 701.874 kb on - strand, within MMJJ_RS03770 at 701.875 kb on + strand, within MMJJ_RS03770 at 701.878 kb on - strand, within MMJJ_RS03770 at 701.878 kb on - strand, within MMJJ_RS03770 at 701.878 kb on - strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.879 kb on + strand, within MMJJ_RS03770 at 701.906 kb on + strand, within MMJJ_RS03770 at 701.921 kb on + strand, within MMJJ_RS03770 at 701.921 kb on + strand, within MMJJ_RS03770 at 701.921 kb on + strand, within MMJJ_RS03770 at 701.924 kb on - strand, within MMJJ_RS03770 at 701.924 kb on - strand, within MMJJ_RS03770 at 701.925 kb on + strand, within MMJJ_RS03770 at 701.942 kb on - strand, within MMJJ_RS03770 at 702.025 kb on - strand, within MMJJ_RS03770 at 702.031 kb on - strand, within MMJJ_RS03770 at 702.032 kb on + strand, within MMJJ_RS03770 at 702.032 kb on + strand, within MMJJ_RS03770 at 702.053 kb on - strand, within MMJJ_RS03770 at 702.053 kb on - strand, within MMJJ_RS03770 at 702.054 kb on + strand, within MMJJ_RS03770 at 702.094 kb on - strand at 702.095 kb on + strand at 702.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 699,773 + MMJJ_RS03745 0.75 -0.7 699,773 + MMJJ_RS03745 0.75 +0.5 699,849 + MMJJ_RS03745 0.85 +0.7 699,854 - MMJJ_RS03745 0.86 +0.2 699,855 + MMJJ_RS03745 0.86 -1.5 699,866 - MMJJ_RS03745 0.87 -0.7 699,935 + +0.7 699,938 - +0.7 699,939 + -2.0 699,939 + -0.1 699,942 - -1.2 699,956 - +1.1 699,957 + +1.3 699,964 + +0.2 699,977 - +1.3 699,979 - -0.9 699,980 + +0.6 700,004 - -0.3 700,019 + -0.4 700,025 - -0.1 700,026 + +0.3 700,049 + MMJJ_RS03750 0.17 -1.3 700,057 + MMJJ_RS03750 0.22 +0.4 700,060 - MMJJ_RS03750 0.25 +0.8 700,060 - MMJJ_RS03750 0.25 -0.4 700,060 - -0.5 700,060 - MMJJ_RS03750 0.25 +0.2 700,060 - MMJJ_RS03750 0.25 -0.8 700,060 - MMJJ_RS03750 0.25 -0.2 700,060 - MMJJ_RS03750 0.25 -0.0 700,060 - MMJJ_RS03750 0.25 -0.0 700,060 - MMJJ_RS03750 0.25 -1.4 700,060 - MMJJ_RS03750 0.25 -0.4 700,060 - MMJJ_RS03750 0.25 -1.5 700,060 - MMJJ_RS03750 0.25 +1.0 700,060 - MMJJ_RS03750 0.25 -0.5 700,061 + MMJJ_RS03750 0.25 +0.6 700,061 + MMJJ_RS03750 0.25 -0.4 700,061 + MMJJ_RS03750 0.25 -0.0 700,061 + MMJJ_RS03750 0.25 -0.2 700,061 + MMJJ_RS03750 0.25 +0.2 700,061 + MMJJ_RS03750 0.25 -1.0 700,061 + MMJJ_RS03750 0.25 +1.5 700,061 + MMJJ_RS03750 0.25 +0.3 700,061 + MMJJ_RS03750 0.25 -0.5 700,061 + MMJJ_RS03750 0.25 -0.7 700,061 + MMJJ_RS03750 0.25 +0.6 700,061 + MMJJ_RS03750 0.25 +1.1 700,061 + MMJJ_RS03750 0.25 -0.2 700,061 + MMJJ_RS03750 0.25 -0.3 700,061 + -1.3 700,061 + MMJJ_RS03750 0.25 +0.9 700,062 - MMJJ_RS03750 0.26 +0.2 700,062 - MMJJ_RS03750 0.26 +0.0 700,062 - MMJJ_RS03750 0.26 -0.9 700,063 + MMJJ_RS03750 0.27 +0.2 700,162 + +1.9 700,192 + -1.1 700,192 - +1.7 700,209 - -0.8 700,209 - +0.6 700,210 + +1.4 700,210 + +1.6 700,210 + -1.1 700,210 + +0.7 700,210 + +0.2 700,214 - +1.0 700,215 + +1.0 700,218 - -1.0 700,219 + -0.5 700,219 + -2.0 700,256 - MMJJ_RS03755 0.20 -0.4 700,281 - MMJJ_RS03755 0.27 +0.9 700,281 - MMJJ_RS03755 0.27 +1.4 700,282 + MMJJ_RS03755 0.27 -0.7 700,282 + MMJJ_RS03755 0.27 +0.7 700,446 + MMJJ_RS03755 0.73 +0.9 700,446 + MMJJ_RS03755 0.73 -0.4 700,499 - MMJJ_RS03755 0.88 -0.1 700,523 - -1.0 700,589 + +0.4 700,596 - -0.9 700,598 - +0.2 700,598 - +0.9 700,598 - -0.8 700,599 + +0.6 700,600 - +0.8 700,601 + -0.5 700,601 + +1.6 700,601 + -1.2 700,604 + +1.1 700,635 - +0.5 700,636 + +0.8 700,681 - -0.0 700,685 - -0.0 700,685 - +0.0 700,685 - -0.4 700,695 - -0.7 700,695 - -0.3 700,695 - +0.8 700,695 - +0.6 700,696 + -0.9 700,696 + +0.4 700,696 + -0.3 700,696 + -1.3 700,696 + -0.5 700,697 - +0.6 700,698 + -3.9 700,698 + -0.2 700,698 + +0.9 700,698 + +0.1 700,698 + -1.3 700,712 + +0.8 700,727 + +1.2 700,727 + -0.8 700,730 - -0.5 700,731 + +0.1 700,778 - -2.5 700,808 + MMJJ_RS03760 0.17 -2.5 700,835 - +0.5 700,835 - MMJJ_RS03760 0.24 -2.9 700,863 + MMJJ_RS03760 0.32 -2.1 700,942 - MMJJ_RS03760 0.52 -0.6 700,946 + MMJJ_RS03760 0.54 -2.8 700,991 - MMJJ_RS03760 0.65 -3.0 701,021 - MMJJ_RS03760 0.73 -2.3 701,022 + MMJJ_RS03760 0.73 -2.0 701,023 - MMJJ_RS03760 0.74 -3.3 701,031 + -1.0 701,261 - MMJJ_RS03765 0.30 -0.3 701,261 - MMJJ_RS03765 0.30 -2.0 701,261 - MMJJ_RS03765 0.30 -0.6 701,262 + MMJJ_RS03765 0.30 +1.5 701,262 + MMJJ_RS03765 0.30 -0.5 701,285 + MMJJ_RS03765 0.35 -0.8 701,285 + MMJJ_RS03765 0.35 -1.9 701,288 - MMJJ_RS03765 0.36 -2.9 701,288 - MMJJ_RS03765 0.36 -2.3 701,289 + MMJJ_RS03765 0.36 -1.8 701,339 + MMJJ_RS03765 0.47 -1.5 701,339 + MMJJ_RS03765 0.47 -0.8 701,370 - MMJJ_RS03765 0.54 -0.1 701,373 - MMJJ_RS03765 0.54 -0.8 701,374 + MMJJ_RS03765 0.55 -2.1 701,374 + MMJJ_RS03765 0.55 -2.3 701,375 - MMJJ_RS03765 0.55 -2.4 701,375 - MMJJ_RS03765 0.55 -2.1 701,376 + MMJJ_RS03765 0.55 -2.0 701,379 - MMJJ_RS03765 0.56 -2.1 701,574 - -0.8 701,600 + -2.8 701,602 + +1.0 701,602 + +1.3 701,602 + +0.0 701,602 + +0.4 701,602 + -1.8 701,606 - +0.3 701,637 - -0.1 701,637 - +0.1 701,637 - -1.0 701,638 + +1.2 701,638 + -3.1 701,638 + +0.2 701,639 - +0.3 701,640 + -1.0 701,640 + +0.4 701,641 - -0.8 701,650 - -1.1 701,651 + +0.4 701,652 - -0.8 701,655 - +0.0 701,664 + +0.7 701,711 - +0.1 701,711 - +0.5 701,712 + +0.8 701,715 - +0.9 701,715 - -2.5 701,716 + -0.5 701,775 + -1.3 701,784 - -1.4 701,814 + -0.2 701,839 - -0.9 701,844 - -0.5 701,866 - +0.5 701,867 + +1.0 701,874 - MMJJ_RS03770 0.12 -0.4 701,874 - MMJJ_RS03770 0.12 +1.0 701,874 - MMJJ_RS03770 0.12 +0.4 701,875 + MMJJ_RS03770 0.13 +0.7 701,878 - MMJJ_RS03770 0.14 -0.4 701,878 - MMJJ_RS03770 0.14 -0.0 701,878 - MMJJ_RS03770 0.14 -0.4 701,879 + MMJJ_RS03770 0.14 +0.6 701,879 + MMJJ_RS03770 0.14 -2.0 701,879 + MMJJ_RS03770 0.14 +0.8 701,879 + MMJJ_RS03770 0.14 -0.3 701,879 + MMJJ_RS03770 0.14 -0.5 701,879 + MMJJ_RS03770 0.14 -0.5 701,879 + MMJJ_RS03770 0.14 +1.0 701,879 + MMJJ_RS03770 0.14 +0.7 701,879 + MMJJ_RS03770 0.14 +1.0 701,906 + MMJJ_RS03770 0.25 +1.2 701,921 + MMJJ_RS03770 0.31 -1.5 701,921 + MMJJ_RS03770 0.31 +1.4 701,921 + MMJJ_RS03770 0.31 +0.3 701,924 - MMJJ_RS03770 0.33 -0.6 701,924 - MMJJ_RS03770 0.33 -0.8 701,925 + MMJJ_RS03770 0.33 -0.6 701,942 - MMJJ_RS03770 0.40 +0.8 702,025 - MMJJ_RS03770 0.73 -0.0 702,031 - MMJJ_RS03770 0.76 -2.0 702,032 + MMJJ_RS03770 0.76 +0.9 702,032 + MMJJ_RS03770 0.76 -0.9 702,053 - MMJJ_RS03770 0.84 +1.3 702,053 - MMJJ_RS03770 0.84 +0.5 702,054 + MMJJ_RS03770 0.85 -1.0 702,094 - -1.5 702,095 + -0.3 702,107 + -0.1
Or see this region's nucleotide sequence