Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03665 and MMJJ_RS03670 are separated by 19 nucleotides MMJJ_RS03670 and MMJJ_RS03675 are separated by 77 nucleotides
MMJJ_RS03665: MMJJ_RS03665 - shikimate kinase, at 684,162 to 685,013
_RS03665
MMJJ_RS03670: MMJJ_RS03670 - precorrin-2 C(20)-methyltransferase, at 685,033 to 685,719
_RS03670
MMJJ_RS03675: MMJJ_RS03675 - dihydroxy-acid dehydratase, at 685,797 to 687,449
_RS03675
Position (kb) 685
686 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 684.043 kb on + strand at 684.056 kb on - strand at 684.056 kb on - strand at 684.057 kb on + strand at 684.057 kb on + strand at 684.060 kb on + strand at 684.070 kb on + strand at 684.070 kb on + strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.071 kb on - strand at 684.074 kb on - strand at 684.075 kb on + strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.077 kb on - strand at 684.078 kb on + strand at 684.078 kb on + strand at 684.079 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.082 kb on - strand at 684.083 kb on + strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.085 kb on - strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.086 kb on + strand at 684.098 kb on - strand at 684.099 kb on + strand at 684.099 kb on + strand at 684.103 kb on - strand at 684.983 kb on - strand at 684.984 kb on + strand at 685.012 kb on + strand at 685.169 kb on + strand, within MMJJ_RS03670 at 685.179 kb on + strand, within MMJJ_RS03670 at 685.185 kb on - strand, within MMJJ_RS03670 at 685.267 kb on + strand, within MMJJ_RS03670 at 685.337 kb on + strand, within MMJJ_RS03670 at 685.362 kb on - strand, within MMJJ_RS03670 at 685.363 kb on + strand, within MMJJ_RS03670 at 685.363 kb on + strand, within MMJJ_RS03670 at 685.365 kb on + strand, within MMJJ_RS03670 at 685.377 kb on - strand, within MMJJ_RS03670 at 685.496 kb on - strand, within MMJJ_RS03670 at 685.526 kb on - strand, within MMJJ_RS03670 at 685.579 kb on + strand, within MMJJ_RS03670 at 685.701 kb on - strand at 685.718 kb on + strand at 685.727 kb on - strand at 685.734 kb on - strand at 685.734 kb on - strand at 685.735 kb on + strand at 685.735 kb on + strand at 685.755 kb on + strand at 686.406 kb on + strand, within MMJJ_RS03675
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 684,043 + +0.6 684,056 - -3.0 684,056 - -1.3 684,057 + +0.6 684,057 + +1.0 684,060 + +0.6 684,070 + -1.4 684,070 + +0.5 684,071 - +0.8 684,071 - -0.4 684,071 - -0.5 684,074 - -0.6 684,075 + +0.8 684,077 - -0.4 684,077 - -0.7 684,077 - -0.0 684,077 - -1.1 684,078 + +0.7 684,078 + +0.6 684,079 - +0.9 684,082 - -0.6 684,082 - -0.1 684,082 - +0.7 684,082 - -1.2 684,083 + +0.1 684,085 - +0.1 684,085 - +0.6 684,085 - +0.6 684,086 + +0.9 684,086 + -0.6 684,086 + -1.8 684,086 + -0.8 684,086 + -2.0 684,086 + -0.3 684,098 - -3.1 684,099 + -1.0 684,099 + -0.9 684,103 - -0.5 684,983 - -1.5 684,984 + -1.5 685,012 + -0.2 685,169 + MMJJ_RS03670 0.20 -2.8 685,179 + MMJJ_RS03670 0.21 -2.4 685,185 - MMJJ_RS03670 0.22 -3.1 685,267 + MMJJ_RS03670 0.34 -2.4 685,337 + MMJJ_RS03670 0.44 -4.0 685,362 - MMJJ_RS03670 0.48 -3.3 685,363 + MMJJ_RS03670 0.48 -2.0 685,363 + MMJJ_RS03670 0.48 -2.8 685,365 + MMJJ_RS03670 0.48 -3.0 685,377 - MMJJ_RS03670 0.50 -1.8 685,496 - MMJJ_RS03670 0.67 -2.7 685,526 - MMJJ_RS03670 0.72 -2.1 685,579 + MMJJ_RS03670 0.79 -3.3 685,701 - -3.1 685,718 + +0.4 685,727 - +0.2 685,734 - +1.0 685,734 - +1.8 685,735 + -1.5 685,735 + -0.1 685,755 + -0.5 686,406 + MMJJ_RS03675 0.37 +0.0
Or see this region's nucleotide sequence