Experiment: H2 N-free with 10mM Ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS03565 and MMJJ_RS03570 are separated by 27 nucleotides MMJJ_RS03570 and MMJJ_RS03575 are separated by 110 nucleotides MMJJ_RS03575 and MMJJ_RS03580 are separated by 120 nucleotides MMJJ_RS03580 and MMJJ_RS03585 are separated by 29 nucleotides MMJJ_RS03585 and MMJJ_RS03590 are separated by 16 nucleotides MMJJ_RS03590 and MMJJ_RS03595 are separated by 47 nucleotides
MMJJ_RS03565: MMJJ_RS03565 - tRNA-Glu, at 667,897 to 667,971
_RS03565
MMJJ_RS03570: MMJJ_RS03570 - tRNA-Arg, at 667,999 to 668,076
_RS03570
MMJJ_RS03575: MMJJ_RS03575 - NUDIX hydrolase, at 668,187 to 668,702
_RS03575
MMJJ_RS03580: MMJJ_RS03580 - TIGR00303 family protein, at 668,823 to 669,875
_RS03580
MMJJ_RS03585: MMJJ_RS03585 - methanogenesis marker 6 protein, at 669,905 to 670,351
_RS03585
MMJJ_RS03590: MMJJ_RS03590 - restriction endonuclease, at 670,368 to 670,775
_RS03590
MMJJ_RS03595: MMJJ_RS03595 - hypothetical protein, at 670,823 to 671,236
_RS03595
Position (kb) 668
669
670 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 667.841 kb on - strand at 667.841 kb on - strand at 667.842 kb on + strand at 667.842 kb on + strand at 667.980 kb on + strand at 668.149 kb on + strand at 668.165 kb on - strand at 668.166 kb on + strand at 668.166 kb on + strand at 668.173 kb on + strand at 668.174 kb on - strand at 668.179 kb on - strand at 668.179 kb on - strand at 668.181 kb on - strand at 668.182 kb on + strand at 668.182 kb on + strand at 668.182 kb on + strand at 668.196 kb on - strand at 668.196 kb on - strand at 668.197 kb on + strand at 668.197 kb on + strand at 668.198 kb on - strand at 668.198 kb on - strand at 668.198 kb on - strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.199 kb on + strand at 668.201 kb on + strand at 668.217 kb on - strand at 668.218 kb on + strand at 668.256 kb on - strand, within MMJJ_RS03575 at 668.270 kb on - strand, within MMJJ_RS03575 at 668.270 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.274 kb on - strand, within MMJJ_RS03575 at 668.307 kb on - strand, within MMJJ_RS03575 at 668.348 kb on - strand, within MMJJ_RS03575 at 668.390 kb on - strand, within MMJJ_RS03575 at 668.409 kb on + strand, within MMJJ_RS03575 at 668.487 kb on - strand, within MMJJ_RS03575 at 668.548 kb on - strand, within MMJJ_RS03575 at 668.549 kb on + strand, within MMJJ_RS03575 at 668.552 kb on - strand, within MMJJ_RS03575 at 668.571 kb on - strand, within MMJJ_RS03575 at 668.572 kb on + strand, within MMJJ_RS03575 at 668.649 kb on - strand, within MMJJ_RS03575 at 668.688 kb on - strand at 668.689 kb on + strand at 668.693 kb on - strand at 668.693 kb on - strand at 668.693 kb on - strand at 668.702 kb on - strand at 668.741 kb on - strand at 668.742 kb on + strand at 668.746 kb on - strand at 668.764 kb on + strand at 668.764 kb on + strand at 668.775 kb on - strand at 668.775 kb on - strand at 668.776 kb on + strand at 668.776 kb on + strand at 668.780 kb on - strand at 668.781 kb on + strand at 668.781 kb on + strand at 668.781 kb on + strand at 668.794 kb on - strand at 668.794 kb on - strand at 668.794 kb on - strand at 668.794 kb on - strand at 668.795 kb on + strand at 668.795 kb on + strand at 668.796 kb on - strand at 668.797 kb on + strand at 668.797 kb on + strand at 668.797 kb on + strand at 668.797 kb on + strand at 668.799 kb on - strand at 668.799 kb on - strand at 668.800 kb on + strand at 668.800 kb on + strand at 668.800 kb on + strand at 668.800 kb on + strand at 668.803 kb on - strand at 668.803 kb on - strand at 668.810 kb on + strand at 668.858 kb on + strand at 668.898 kb on + strand at 668.922 kb on - strand at 668.922 kb on - strand at 668.922 kb on - strand at 668.923 kb on + strand at 668.995 kb on - strand, within MMJJ_RS03580 at 669.239 kb on - strand, within MMJJ_RS03580 at 669.245 kb on - strand, within MMJJ_RS03580 at 669.255 kb on + strand, within MMJJ_RS03580 at 669.260 kb on + strand, within MMJJ_RS03580 at 669.263 kb on + strand, within MMJJ_RS03580 at 669.339 kb on + strand, within MMJJ_RS03580 at 669.736 kb on + strand, within MMJJ_RS03580 at 669.829 kb on + strand at 669.850 kb on - strand at 669.851 kb on + strand at 669.968 kb on - strand, within MMJJ_RS03585 at 670.291 kb on - strand, within MMJJ_RS03585 at 670.336 kb on + strand at 670.336 kb on + strand at 670.336 kb on + strand at 670.349 kb on - strand at 670.350 kb on + strand at 670.376 kb on - strand at 670.377 kb on + strand at 670.381 kb on + strand at 670.394 kb on - strand at 670.394 kb on - strand at 670.477 kb on - strand, within MMJJ_RS03590 at 670.508 kb on - strand, within MMJJ_RS03590 at 670.545 kb on - strand, within MMJJ_RS03590 at 670.546 kb on + strand, within MMJJ_RS03590 at 670.626 kb on + strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.639 kb on - strand, within MMJJ_RS03590 at 670.644 kb on + strand, within MMJJ_RS03590 at 670.645 kb on - strand, within MMJJ_RS03590 at 670.646 kb on + strand, within MMJJ_RS03590 at 670.745 kb on - strand at 670.773 kb on - strand at 670.773 kb on - strand at 670.774 kb on + strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.776 kb on - strand at 670.777 kb on + strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.778 kb on - strand at 670.779 kb on + strand at 670.779 kb on + strand at 670.792 kb on + strand at 670.839 kb on + strand at 670.866 kb on + strand, within MMJJ_RS03595 at 670.866 kb on + strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.869 kb on - strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595 at 670.870 kb on + strand, within MMJJ_RS03595
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Ammonium chloride remove 667,841 - -0.4 667,841 - +0.1 667,842 + -2.1 667,842 + +1.5 667,980 + -1.2 668,149 + -0.5 668,165 - +1.1 668,166 + +0.2 668,166 + -0.9 668,173 + -0.8 668,174 - -2.5 668,179 - +2.2 668,179 - -0.2 668,181 - +1.1 668,182 + -0.3 668,182 + -0.5 668,182 + -0.7 668,196 - -3.2 668,196 - -0.1 668,197 + +1.8 668,197 + -1.3 668,198 - +0.9 668,198 - -2.8 668,198 - -0.1 668,199 + -1.0 668,199 + -2.3 668,199 + -0.5 668,199 + -0.0 668,199 + -0.8 668,199 + +0.1 668,199 + +0.5 668,199 + -1.1 668,201 + -1.3 668,217 - +1.0 668,218 + +1.3 668,256 - MMJJ_RS03575 0.13 +0.2 668,270 - MMJJ_RS03575 0.16 -0.5 668,270 - MMJJ_RS03575 0.16 +0.3 668,274 - MMJJ_RS03575 0.17 -0.8 668,274 - MMJJ_RS03575 0.17 -0.4 668,274 - MMJJ_RS03575 0.17 +0.8 668,274 - MMJJ_RS03575 0.17 +0.1 668,307 - MMJJ_RS03575 0.23 -1.4 668,348 - MMJJ_RS03575 0.31 -0.7 668,390 - MMJJ_RS03575 0.39 -0.1 668,409 + MMJJ_RS03575 0.43 +0.7 668,487 - MMJJ_RS03575 0.58 -1.7 668,548 - MMJJ_RS03575 0.70 -0.4 668,549 + MMJJ_RS03575 0.70 -0.7 668,552 - MMJJ_RS03575 0.71 +0.5 668,571 - MMJJ_RS03575 0.74 -0.2 668,572 + MMJJ_RS03575 0.75 -1.5 668,649 - MMJJ_RS03575 0.90 -0.8 668,688 - -1.0 668,689 + +0.4 668,693 - +0.2 668,693 - +2.5 668,693 - +0.4 668,702 - -0.5 668,741 - +0.8 668,742 + -1.1 668,746 - -0.3 668,764 + -0.3 668,764 + -0.8 668,775 - +1.0 668,775 - +0.1 668,776 + +0.1 668,776 + -1.0 668,780 - +0.8 668,781 + +0.8 668,781 + -0.2 668,781 + +1.3 668,794 - +0.5 668,794 - -2.3 668,794 - -1.3 668,794 - -1.8 668,795 + +1.0 668,795 + +0.6 668,796 - +0.5 668,797 + +0.1 668,797 + +0.5 668,797 + +1.2 668,797 + -1.5 668,799 - -1.4 668,799 - -0.5 668,800 + +0.8 668,800 + -0.1 668,800 + +1.5 668,800 + +1.3 668,803 - +0.7 668,803 - -0.7 668,810 + -1.2 668,858 + -3.0 668,898 + -1.8 668,922 - -2.8 668,922 - -2.3 668,922 - -2.1 668,923 + -2.8 668,995 - MMJJ_RS03580 0.16 -2.8 669,239 - MMJJ_RS03580 0.40 -2.5 669,245 - MMJJ_RS03580 0.40 -2.1 669,255 + MMJJ_RS03580 0.41 -1.8 669,260 + MMJJ_RS03580 0.42 -3.4 669,263 + MMJJ_RS03580 0.42 -2.3 669,339 + MMJJ_RS03580 0.49 -0.5 669,736 + MMJJ_RS03580 0.87 -3.3 669,829 + +0.0 669,850 - -0.9 669,851 + -1.0 669,968 - MMJJ_RS03585 0.14 -2.3 670,291 - MMJJ_RS03585 0.86 -0.9 670,336 + -1.8 670,336 + -0.2 670,336 + -0.1 670,349 - +0.3 670,350 + +0.2 670,376 - -0.7 670,377 + +1.4 670,381 + +1.5 670,394 - -1.8 670,394 - +0.8 670,477 - MMJJ_RS03590 0.27 +0.3 670,508 - MMJJ_RS03590 0.34 -0.5 670,545 - MMJJ_RS03590 0.43 -1.2 670,546 + MMJJ_RS03590 0.44 +0.4 670,626 + MMJJ_RS03590 0.63 -1.6 670,639 - MMJJ_RS03590 0.66 +0.7 670,639 - MMJJ_RS03590 0.66 -0.6 670,644 + MMJJ_RS03590 0.68 +0.6 670,645 - MMJJ_RS03590 0.68 +1.1 670,646 + MMJJ_RS03590 0.68 -2.2 670,745 - -0.5 670,773 - -0.5 670,773 - +1.0 670,774 + +0.5 670,776 - +0.8 670,776 - -1.2 670,776 - -0.9 670,776 - -1.3 670,777 + -0.8 670,778 - +2.2 670,778 - -0.5 670,778 - +0.9 670,779 + -0.9 670,779 + +0.6 670,792 + +0.2 670,839 + +1.9 670,866 + MMJJ_RS03595 0.10 -1.3 670,866 + MMJJ_RS03595 0.10 -0.8 670,869 - MMJJ_RS03595 0.11 -2.2 670,869 - MMJJ_RS03595 0.11 +0.6 670,869 - MMJJ_RS03595 0.11 +0.7 670,870 + MMJJ_RS03595 0.11 -1.5 670,870 + MMJJ_RS03595 0.11 -0.2
Or see this region's nucleotide sequence